The effect of Tn916 insertions on contour-clamped homogeneous electrophoresis patterns of Enterococcus faecalis

Journal of Clinical Microbiology

Volumn 35, Issue 4, 1997, Pages 969-972

  Thal, L A ^a   Silverman, J ^b   Donabedian, S ^a   Zervos, M J ^a,b,c  

^a WILLIAM BEAUMONT HOSPITAL   (United States)

^b WAYNE STATE UNIVERSITY   (United States)

^c WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; DNA FRAGMENT;

ARTICLE; BACTERIUM IDENTIFICATION; CHROMOSOME BANDING PATTERN; DNA DETERMINATION; ELECTROPHORESIS; ENTEROCOCCUS FAECALIS; NONHUMAN; PRIORITY JOURNAL; STRAIN DIFFERENCE;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS FAECALIS; STREPTOCOCCUS;

EID: 0030949519     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.4.969-972.1997     Document Type: Article

References (21)

1. Chow, J. W., A. Kuritza, D. M. Shlaes, M. Green, D. F. Sahm, and M. J. Zervos. 1993. Clonal spread of vancomycin-resistant Enterococcus faecium between patients in three hospitals in two states. J. Clin. Microbiol. 31:1609-1611.
2. Chow, J. W., M. B. Perri, L. A. Thal, and M. J. Zervos. 1993. Mobilization of the penicillinase gene in Enterococcus faecalis. Antimicrob. Agents Chemother. 37:1187-1189.
3. Clewell, D. B. 1981. Plasmids, drug resistance and gene transfer in the genus Streptococcus. Microbiol. Rev. 45:409-436.
4. Clewell, D. B., and S. E. Flannegan. 1993. In D. B. Clewell (ed.), Bacterial conjugation. The conjugative transposons of gram-positive bacteria. Plenum Press, New York, N.Y.
5. Dembry, L. M., J. A. Vazquez, and M. J. Zervos. 1994. DNA analysis in the study of the epidemiology of nosocomial candidiasis. Infect. Control. Hosp. Epidemiol. 15:48-53.
6. Donabedian, S. M., J. W. Chow, J. M. Boyce, R. E. McCabe, S. M. Markowitz, P. E. Coudron, A. K. Kuritza, C. L. Pierson, and M. J. Zervos. 1992. Molecular typing of ampicillin-resistant, non-β-lactamase-producing Enterococcus faecium isolates from diverse geographic areas. J. Clin Microbiol. 30:2757-2761.
7. Forbes, B. A., and D. R. Schaberg. 1983. Transfer of resistance plasmids from Staphylococcus epidermidis to Staphylococcus aureus: evidence for conjugative exchange of resistance. J. Bacteriol. 153:627-634.
8. Franke, A. E., and D. B. Clewell. 1981. Evidence for a chromosome-borne resistance lransposon in Streptococcus faecalis capable of "conjugal" transfer in the absence of a conjugative plasmid. J. Bacteriol. 145:494-502.
9. Gawron-Burke, C., and D. B. Clewell. 1982. A transposon in Streptococcus faecalis with fertility properties. Nature 300:281-284.
10. Gawron-Burke, C., and D. B. Clewell. 1984. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning genes from grampositive bacteria. J. Bacteriol. 159:214-221.
11. Goering, R. V. 1993. Molecular epidemiology of nosocomial infection: analysis of chromosomal restriction fragment patterns hy pulsed-field gel electrophoresis. Mol. Hosp. Epidemiol. 14:595-600.
12. Hall, L. M. C. 1994. Are point mutations or DNA rearrangements responsible for the restriction fragment length polymorphisms that are used to type bacteria? Microbiology 140:197-204.
13. Mahayni, R., and M. J. Zervos. 1994. The clinical laboratory's role in hospital infection control. Lab. Med. 25:642-647.
14. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.
15. Miranda, A. G., K. V. Singh, and B. E. Murray. 1991. DNA fingerprinting of Enterococcus faecium by pulsed-field gel clectrophoresis may be a useful epidemiologic tool. J. Clin. Microbiol. 29:2752-2757.
16. Murray, B. E., K. V. Singh, J. D. Heath, B. R. Sharma, and G. M. Weinstock. 1990. Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites J Clin. Microbiol. 28:2059-2063.
17. Patterson, J. E., and M. J. Zervos. 1990. High-level gentamicin resistance in enterococcus: microbiology, genetic basis and epidemiology. Rev Infect. Dis. 12:644-652.
18. Quintiliani, R., Jr., and P. Courvalin. 1996. Conjugal transfer of the vancomycin resistance determinant vanB between enterococci involves the movement of large genetic elements from chromosome to chromosome. FEMS Microbiol. Lett. 119:359-364.
19. Seetulsingh, P. S., J. F. Tomayko, P. E. Coudron, S. M. Markowitz, C. Skinner, K. V. Singh, and B. E. Murray. 1996. Chromosomal DNA restriction endonuclease digestion patterns of β-lactamase-producing Enterococcus faecalis isolates collected from a single hospital over a 7-year period. J. Clin. Microbiol. 34:1892-1896.
20. Smith, C. L., and C. R. Cantor. 1987. Purification, specific fragmentation, and separation of large DNA molecules. Methods Enzymol. 155:449-467.
21. Tenover, F. C., R. D. Arbeit, R. V. Goering, P. A. Mickelsen, B. E. Murray, D. H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria tor bacterial strain typing. J. Clin. Microbiol. 33:2233-2239.