Measurement of nucleic acid concentrations using the DyNA Quant(TM) and the GeneQuant(TM)

BioTechniques

Volumn 22, Issue 6, 1997, Pages 1170-1174

  Teare, J M ^a,c   Islam, R ^a   Flanagan, R ^a   Gallagher, S ^a   Davies, M G ^b   Grabau, C ^a  

^a Amersham Biosciences   (United States)

^b Pharmacia Biotech   (United Kingdom)

^c Hoefer Pharmaeia Biotech   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEIC ACID;

ACCURACY; ARTICLE; DNA BINDING; FLUORESCENCE; MEASUREMENT; NUCLEIC ACID ANALYSIS; SPECTROPHOTOMETRY;

EID: 0030949150     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97226pf02     Document Type: Article

References (14)

1. Cesarone, C.F., C. Bolognesi and L. Santi. 1979. Improved microfluorometric DNA determination in biological material using 33258 Hoechst. Anal. Biochem. 100:188-197.
2. Dawson, R.M.C., D.C. Elliott, W.H. Elliott and K.M. Jones. 1986. Data for Biochemical Research. Oxford University Press, New York.
3. Gallagher S.R. 1989. Quantitation of DNA and RNA with absorption and fluorescence spectroscopy, p. A.3.9-A.3.15. In F.A. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current Protocols in Molecular Biology. John Wiley & Sons, New York.
4. Gallagher, S. and Z. Rymaszewski. 1994. Methods in Toxicology, Vol IB, p. 164-177. Academic Press, New York.
5. Glasel, J.A. 1995. Validity of nucleic acid purities monitored by 260nm/280nm absorbance ratios. BioTechniques 18:62-63.
6. Labarca, C. and K. Paigen. 1980. A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102:344-352.
7. Lovett, M., J. Kere and L.M. Hinton. 1991. Direct selection: a method for the isolation of cDNAs encoded by large genomic regions. Proc. Natl. Acad. Sci. USA 88:9628-9632.
8. Miki, Y., J. Swensen, D. Shattuck-Eidens, P.A. Futreal, K. Harshman, S. Tavtigian, Q. Liu, C. Cochran, L.M. Bennett, W. Ding et al. 1994. A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science 266:66-71.
9. Moe D., C. Garbarsch and S. Kireby. 1994. The protein effect on determination of DNA with Hoechst 33258. J. Biochem. Biophys. Methods
10. Pharmacia Biotech. 1996. DyNA Quant 200 Application Note #8, Fluorescence quantitation of commonly used plasmid DNAs using calf thymus DNA as a calibration standard. Product Code 80-6323-58. Piscataway, NJ.
11. Pharmacia Biotech. 1996. DyNA Quant 200 Application Note #10, Fluorescence quantitation of PCR products using the DyNA Quant 200 prior to re-amplification and direct sequencing. Product Code 80-6338-59. Piscataway, NJ.
12. Rymaszewski, Z., W.A. Abplanalp, R.M. Cohen and P. Chomczynski. 1990. Estimation of cellular DNA content in cell lysates by freezing in distilled water. Anal. Biochem. 188:91-96.
13. Wang J., S. Kim and S. Gallagher. 1994. Effect of A + T content on fluorescent DNA quantitation. Hoefer News 94-4:3. Hoefer Pharmacia Biotech, San Francisco.
14. Warburg, O. and W. Christian. 1942. Isolation and crystallization of enolase. Biochem. Z. 310:384-421.