The efflux of lysine from the basolateral membrane of human cultured intestinal cells (Caco-2) occurs by different mechanisms depending on the extracellular availability of amino acids

Journal of Nutrition

Volumn 127, Issue 6, 1997, Pages 1183-1190

  Ferruzza, Simonetta ^a   Ranaldi, Giulia ^a   Di Girolamo, Mario ^b   Sambuy, Yula ^a  

^a ISTITUTO NAZIONALE DELLA NUTRIZIONE   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Amino acid counter exchange; Basolateral active efflux; Caco 2 human cells; Lysine bioavailability; Trans stimulation

Indexed keywords

LYSINE;

AMINO ACID METABOLISM; ARTICLE; BASOLATERAL MEMBRANE; BIOAVAILABILITY; CELL MEMBRANE TRANSPORT; CELL STRAIN CACO 2; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTESTINE ABSORPTION; INTESTINE EPITHELIUM CELL; PROTEIN TRANSPORT;

STROPHANTHUS GRATUS;

EID: 0030947286     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/127.6.1183     Document Type: Article

References (25)

1. Alvarez-Hemandez, X., Smith, M. & Glass, J. (1994) Regulation of iron uptake and transport by transferrin in Caco-2 cells, an intestinal cell line. Biochim. Biophys. Acta 1192: 215-222.
2. Bertran, J., Testar, X., Zorzano, A. & Palacin, M. (1994) A new age for mammalian plasma membrane amino acid transporters. Cell. Physiol. Biochem. 4: 217-241.
3. +-dependent sugar transport in Caco-2 cells and human fetal colon. J. Membr. Biol. 99: 113-125.
4. Burnham, D. & Fondacaro, J. (1989) Secretagogue-induced protein phosphorylation and chloride transport in Caco-2 cells. Am. J. Physiol. 256: G808G816.
5. Chandler, C. E., Zaccaro, L. M. & Moberley, J. B. (1993) Transepithelial transport of cholyltaurine by Caco-2 cell monolayers is sodium dependent. Am. J. Physiol. 264: G1118-G1125.
6. Cheeseman, C. (1992) Role of intestinal basolateral membrane in absorption of nutrients. Am. J. Physiol. 262: R482-R488.
7. Chen. J., Zhu, Y. & Hu, M. (1994) Mechanisms and kinetics of uptake and efflux of L-methionine in an intestinal epithelial model Caco-2. J. Nutr. 124: 1907-1916.
8. Chen, T. R. (1977) In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp, Cell Res. 104: 255-262.
9. +L-like. J. Biol. Chem. 271: 17761-17770.
10. Eleno, N., Devés, R. & Boyd, C. (1994) Membrane potential dependence of the kinetics of cationic amino acid transport systems in human placenta. J. Physiol. 479: 291-300.
11. +-independent mechanisms on different plasma membrane domains. J. Nutr. 125: 2577-2585.
12. Jacob, R., Rosenthal, N. & Barrett, E. (1986) Characterization of glutamine transport by liver plasma membrane vesicles. Am. J. Physiol. 251: E509E514.
13. Kilberg, M., Handlogten, M. & Christensen, H. (1980) Characteristics of an amino acid transport system in rat liver for glutamine, asparagine, histidine and closely related analogs. J. Biol. Chem. 255: 4011-4019.
14. Kleinbaum, D. & Kupper, L. (1978) Applied Regression Analysis and Other Multivariable Methods. Duxbury Press, Boston, MA.
15. Lawless, K., Maenz, D. & Cheesman, C. (1987) Is leucine an allosteric modulator of the lysine transporter in the intestinal basolateral membrane? Am. J. Physiol. 253: G637-G642.
16. Lowry, O. H., Rosebrough, N.J., Fair, A. L. & Randall, R. J. (1951) Protein measurement with the Polin phenol reagent. J. Biol. Chem. 193: 265-275
17. Maillard, M., Stevens, B. & Mann, G. (1995) Amino acid transport by small intestinal, hepatic, and pancreatic epithelia. Gastroenterology 108: 888-910.
18. Marquardt, D. W. (1963) An algorithm for least squares estimation of non-linear parameters, J. Soc. Ind. Appl. Math. 11: 431-441.
19. Meunier, V., Bourrié, M., Berger, Y. & Fabre, G. (1995) The human intestinal epithelial cell line Caco-2; pharmacological and pharmacokinetic applications. Cell Biol. Toxicol. 11: 187-194.
20. Palacin, M. (1994) A new family of proteins (rBAT and 4F2hc) involved in cationic and zwitterionic amino acid transport: a tale of two proteins in search of a transport function. J. Exp. Biol. 196: 123-137.
21. 4 L-arginine transport capacity in differentiating human intestinal Caco-2 cells. Am. J. Physiol. 268: G278-G585.
22. Ranaldi, G., Islam, K. & Sambuy, Y. (1994) D-Cycloserine uses an active transport mechanism in the human intestinal cell line Caco 2. Antimicrob. Agents Chemother. 38: 1239-1245.
23. Skou, J. (1988) Overview: the Na,K-pump. Methods Enzymol. 156: 1-25.
24. +-independent lysine transport in human intestinal Caco-2 cells. J. Membr. Biol. 151: 215-224.
25. White, M. F. (1985) The transport of cationic amino acids across the plasma membrane of mammalian cells. Biochim. Biophys. Acta 822: 355-374.