A simplified serum-free method for preparation and cultivation of human granulosa-luteal cells

Human Reproduction

Volumn 12, Issue 3, 1997, Pages 523-531

  Figenschau, Y ^a   Sundsfjord, J A ^a   Yousef, M I ^a,b   Fuskevag O M ^c   Sveinbjornsson B ^b   Bertheussen, K ^a  

^a UNIVERSITY HOSPITAL OF NORTH NORWAY   (Norway)

^b UNIVERSITY OF TROMSØ   (Norway)

^c ALEXANDRIA UNIVERSITY   (Egypt)

Author keywords

Cell preparation; Granulosa cell culture; Progesterone; Proliferation; Serum free

Indexed keywords

CHORIONIC GONADOTROPIN; PROGESTERONE;

ADULT; ARTICLE; CELL COUNT; CELL CULTURE; CELL DENSITY; CONTAMINATION; CONTROLLED STUDY; CULTURE MEDIUM; FEMALE; GRANULOSA CELL; HUMAN; HUMAN CELL; LEUKOCYTE; LUTEAL CELL; MONOLAYER CULTURE; PROGESTERONE RELEASE; PROGESTERONE SYNTHESIS;

EID: 0030947170     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/humrep/12.3.523     Document Type: Article

References (40)

1. Albertini, D.F. and Andersen, E. (1974) The appearance and structure of intercellular connections during the ontogeny of the rabbit ovarian follicle with particular reference to gap junctions. J. Cell Biol., 63, 234-250.
2. Amsterdam, A., Rotmensch, S., Furman, A. et al. (1989) Synergistic effect of human chorionic gonadotropin and extracellular matrix on in vitro differentiation of human granulosa cells: progesterone production and gap junction formation. Endocrinology, 124, 1956-1964.
3. Asem, E.K. and Novero, R.P. (1993) Influence of follicular maturation on the deposition of fibronectin by chicken granulosa cells in vitro - effect of 8-bromo cyclic AMP. Domest. Anim. Endocrinol., 10, 219-228.
4. Aston, K.E., Stamouli, A. and Thomas, E.J. (1996a) Effect of gonadotrophin on cell and matrix retention and expression of metalloproteinases and their inhibitor in cultured human granulosa cells modelling corpus luteum function. Mol. Hum. Reprod., 2, 26-30.
5. Aston, K.E., O'Sullivan, M.J.O., Thomas, E.J. et al (1996b) Effect of human chorionic gonadotrophin on the detachment of human granulosa cells from extracellular matrix layered onto glass or plastic. Hum. Reprod., 11, 336-340.
6. Beckmann, M.W., Polacek, D., Seung, L. et al. (1991) Human ovarian granulosa cell culture: determination of blood cell contamination and evaluation of possible purification steps. Fertil. Steril., 56, 881-887.
7. Benyo, D.F. and Pate, J.L. (1992) Tumor necrosis factor-α alters bovine granulosa-luteal cell synthetic capacity and viability. Endocrinology, 130, 854-860.
8. Bertheussen, K. (1993) Growth of cells in a new defined protein-free medium. Cytotechnology, 11, 219-231.
9. Best, C.L., Pudney, J., Anderson, D.J. et al. (1994) Modulation of human granulosa cell steroid production in vitro by tumor necrosis factor α: implications of white blood cells in culture. Obstet. Gynecol., 84, 121-127.
10. Bortolussi, M., Zanchetta, R., Doliana, R. et al (1989) Changes in the organization of the extracellular matrix in ovarian follicles during the preovulatory phase and atresia. An immunofluorescence study. Basic Appl. Histochem., 33, 31-38.
11. Chaffkin, L.M., Luciano, A.A. and Peluso, J.J. (1992) Progesterone as an autocrine/paracrine regulator of human granulosa cell proliferation. J. Clin. Endocrinol. Metab., 75, 1404-1408.
12. Chaffkin, L.M., Luciano, A.A. and Peluso, J.J. (1993) The role of progesterone in regulating human granulosa cell proliferation and differentiation in vitro. J. Clin. Endocrinol. Metab., 76, 696-700.
13. Epstein-Almog, R. and Orly, J. (1985) Inhibition of hormone-induced steroidogenesis during cell proliferation in serum-free cultures of rat granulosa cells. Endocrinology, 116, 2103-2112.
14. Furman, A., Rotmensch, S., Dor, J. et al. (1986) Culture of human granulosa cells from an in vitro fertilization program: effects of extracellular matrix on morphology and cyclic adenosine 3′,5′ monophosphate production. Fertil. Steril., 46, 514-517.
15. Harper, E. and Kang, A.H. (1970) Studies on the specificity of bacterial collagenase. Biochem. Biophys. Res. Commun., 41, 482-487.
16. Holst, N., Jacobsen, M.B., Haug, E. et al. (1995) Somatostatin in physiological concentrations inhibits basal and enhanced luteinizing hormone-stimulated progesterone release from human granulosa-luteal cells. Hum. Reprod., 10, 1363-1366.
17. Holst, N., Bertheussen, K., Burhol, P.G. et al. (1991) Medium-associated luteinization expressed as progesterone release in granulosa-luteal cells isolated from patients undergoing in-vitro fertilization. Hum. Reprod., 6, 1343-1348.
18. Inkster, S.E. and Brodie, A.M. (1991) Expression of aromatase cytochrome P-450 in premenopausal and postmenopausal human ovaries: an immunocytochemical study. J. Clin. Endocrinol. Metab., 73, 717-726.
19. Kasson, B.C., Meidan, R., Davoren, J.B. et al (1985) Identification of subpopulations of rat granulosa cells: sedimentation properties and hormonal responsiveness. Endocrinology, 117, 1027-1034.
20. Krieger, M., Kay, L.M. and Stroud, R.M. (1974) Structure and specific binding of trypsin: comparison of inhibited derivatives and a model for substrate binding. J. Mol. Biol., 83, 209-230.
21. Memon, R.A., Mohan, C., Geiger, P.J. et al. (1989) Lack of insulin stimulation on Percoll-prepared or high-speed centrifuged rat liver hepatocytes. Biochem. Med. Metab. Biol., 42, 216-219.
22. Mestwerdt, W. and Müller, O. (1982) Morphology and morphometry of human preovulatory follicles collected during LH surge with emphasis on granulosa cells. In Hafez, E.S.E. and Semm, K. (eds), In Vitro Fertilization and Embryo Transfer. MTP Press Ltd, Lancaster, UK, p. 233.
23. Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65, 55-63.
24. Nie, Z. (1992) Fetuin: its enigmatic property of growth promotion. Am. J. Physiol., 263, C551-C562.
25. Novero, R.P. and Asem, E.K. (1993) Follicle-stimulating hormone-enhanced fibronectin production by chicken granulosa cells is influenced by follicular development. Poultry Sci., 72, 709-721.
26. Ohara, A., Mori, T., Taii, S. et al. (1987) Functional differentiation in steroidogenesis of two types of luteal cells isolated from mature human corpora lutea of the menstrual cycle. J. Clin. Endocrinol. Metab., 65, 1192-1200.
27. Olsson, J.H., Carlsson, B. and Hillensjo, T. (1990) Effect of insulin-like growth factor I on deoxyribonucleic acid synthesis in cultured human granulosa cells. Fertil. Steril., 54, 1052-1057. Erratum: (1991) Fertil. Steril., 55, 654.
28. Pickering, S.J., Fleming, T.P., Braude, P.R. et al. (1989) Are human spermatozoa separated on a Percoll density gradient safe for therapeutic use? Fertil. Steril., 51, 1024-1029.
29. Rao, I.M., Mills, T.M., Anderson, E. et al. (1991) Heterogeneity in granulosa cells of developing rat follicles. Anat. Rec., 229, 177-185.
30. Salustri, A., Yanagishita, M., Underhill, C.B. et al. (1992) Localization and synthesis of hyaluronic acid in the cumulus cells and mural granulosa cells of the preovulatory follicle. Dev. Biol., 151, 541-551.
31. Sipos, T. and Merkel, J.R. (1970) An effect of calcium ions on the activity, heat stability, and structure of trypsin. Biochemistry, 9, 2766-2775.
32. Sprang, S., Standing, T, Fletterick, R.J. et al. (1987) The three-dimensional structure of the Asn102 mutant of trypsin: role of Asp102 in serine protease catalysis. Science, 237, 905-909.
33. Tapanainen, J., Leinonen, P.J., Tapanainen, P. et al. (1987) Regulation of human granulosa-luteal cell progesterone production and proliferation by gonadotropins and growth factors. Fertil. Steril., 48, 576-580.
34. Uhlin Hansen, L. and Yanagishita, M. (1993) Differential effect of brefeldin A on the biosynthesis of heparan sulfate and chondroitin/dermatan sulfate proteoglycans in rat ovarian granulosa cells in culture. J. Biol. Chem., 268, 17370-17376.
35. Wakim, A.N., Polizotto, S.L. and Burholt, D.R. (1996) Augmentation by thyroxine of human granulosa cell gonadotrophin-induced steroidogenesis. Hum. Reprod., 10, 2845-2848.
36. Whitman, G.F., Boldt, J.P., Martinez, J.E. and Pantazis, C.G. (1991) Flow cytometric analysis of induced human Graafian follicles. I. Demonstration and sorting of two luteinized cell populations. Fertil. Steril., 56, 259-265.
37. Yamamoto, K. and Sinohara, H. (1993) Isolation and characterization of mouse countertrypsin, a new trypsin inhibitor belonging to the mammalian fetuin family. J. Biol. Chem., 268, 17750-17753.
38. Yan, Z., Hunter, V., Weed, J. et al. (1993) Tumor necrosis factor-α alters steroidogenesis and stimulates proliferation of human ovarian granulosa cells in vitro. Fertil. Steril., 59, 332-338.
39. Yasuda, K., Fukuoka, M., Fujiwara, H. et al. (1992) Effects of Interferon on the steroidogenic functions and proliferation of immature porcine granulosa cells in culture. Biol. Reprod., 47, 931-936.
40. Yie, S.-M., Brown, G.M., Liu, G.-Y. et al. (1995) Melatonine and steroids in human pre-ovulatory follicular fluid: seasonal variations and granulosa cell steroid production. Hum. Reprod., 10, 50-55.