The most prevalent protein in a heat-treated extract of pea (Pisum sativum) embryos is an LEA group I protein; its conformation is not affected by exposure to high temperature

Seed Science Research

Volumn 7, Issue 2, 1997, Pages 117-123

  Russouw, Pauline S ^a   Farrant, Jill ^a   Brandt, Wolf ^a   Lindsey, George G ^a  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Denaturation; Embryos; Heat; LEA protein; Peas

Indexed keywords

PISUM SATIVUM; SATIVUM;

EID: 0030946085     PISSN: 09602585     EISSN: None     Source Type: Journal    
DOI: 10.1017/s0960258500003457     Document Type: Conference Paper

References (26)

1. Baker, J., Steele, C. and Dure, L., III (1988) Sequence and characterization of 6 LEA proteins and their genes from cotton. Plant Molecular Biology 11, 277-291.
2. Blackman, S.A., Wettlaufer, S.H., Obendorf, R.L. and Leopold, A.C. (1991) Maturation proteins associated with desiccation tolerance in soybean. Plant Physiology 96, 868-874.
3. Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1995) Desiccation tolerance in developing soybean seeds: the role of stress proteins. Physiologia Plantarium 93, 630-638.
4. Bradford, K.J. and Chandler, P.M. (1992) Expression of "dehydrin-like" proteins in embryos and seedlings of Zizania palustris and Oryza sativa during dehydration. Plant Physiology 99, 488-494.
5. Brand, L. and Witholt, B. (1967) Fluorescence measurements. Methods in Enzymology 11, 776-856.
6. Brandt, W.F., Alk, H., Chauhan, M. and von Holt, C. (1984) A simple modification converts the spinning cup sequencer into a vapour phase sequencer. FEBS Letters 174, 228-232.
7. Close, T.J., Kort, A.A. and Chandler, P.M. (1989) A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Molecular Biology 13, 95-108.
8. Close, T.J., Fenton, R.D. and Moonan, E (1993) A view of plant dehydrins using antibodies specific to the carboxy terminal peptide. Plant Molecular Biology 23, 279-286.
9. Dure, L., III, Crouch, M., Harada, J., Ho, T.H.D., Mundy, J., Quatrano, R., Thomas, T. and Sung, Z.R. (1989) Common amino acid sequence domains among the LEA proteins of higher plants. Plant Molecular Biology 12, 475-486,
10. Espelund, M., Saebøe-Larssen, S., Hughes, D.W., Galau, G.A., Larsen, F. and Jakobsen, K.S. (1992) Late embryogenesis-abundant genes encoding proteins with different numbers of hydrophilic repeats are regulated differentially by abscisic acid and osmotic stress. Plant Journal 2, 241-252.
11. Galau, G.A., Hughes, D.W. and Dure, L., III. (1986) Abscisic acid induction of cloned cotton late embryogenesis-abundant (LEA) mRNAs. Plant Molecular Biology 7, 157-170.
12. Galau, G.A., Wang, H.Y.C. and Hughes, D.W. (1992) Cotton Lea4 (D19) and LeaA2 (D132) Group 1 Lea genes encoding water stress-related proteins containing a 20-amino acid motif. Plant Physiology 99, 783-788.
13. Guagliardi, A., Cerchia, L., Bartolucci, S. and Rossi, M. (1994) The chaperonin from the archeon Sulfolobus solfactances promotes correct refolding and prevents thermal denaturation in vitro. Protein Science 3, 1436-1443.
14. Harlow, E. and Lane, D, (1988) Antibodies - a laboratory manual. Cold Spring Harbour Laboratory.
15. Hewick, R.M., Hunkapiller, M.W., Hood, L.E. and Dreyer, W.J. (1981) A gas-liquid solid phase peptide and protein sequencer. Journal of Biological Chemistry 256, 7990-7997.
16. Ingram, I. and Bartels, D. (1996) The molecular basis of dehydration tolerance in plants. Annual Review of Plant Physiology and Plant Molecular Biology 47, 377-403.
17. Jepson, S.G. and Close, T.J. (1995) Purification of a maize dehydrin protein expressed in Escherichia coli. Protein Expression and Purification 6, 632-636.
18. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature 227, 680-685.
19. Lindsey, G.G., Thompson, P., Pretorias, L., Purves, L.R. and von Holt, C. (1982) The effect of calcium ions on the stability of chicken erythrocyte his tone octamers. FEBS Letters 149, 277-280.
20. Lindsey, G.G., Thompson, P., Pretorias, L., Purves, L.R. and von Holt, C. (1983) Octamer reconstitution from acid extracted chicken erythrocyte his tones FEBS Letters 155, 301-305.
21. Lottspiech, F. (1985) Microscale isocratic separation of the phenylthiohydantoin amino acid derivatives. Journal of Chromatography 326, 321-327.
22. McCubbin, W.D. and Kay, CM. (1985) Hydrodynamic and optical properties of the wheat germ Em protein. Canadian Journal of Biochemistry and Cell Biology 63, 803-811.
23. Mendoza, J.A., Lorimer, G.H. and Horowitz, P.M. (1992) Chaperonin cpn60 from Escherichia coli protects the mitochondrial enzyme rhodanese against heat inactivation and supports folding at elevated temperatures. Journal of Biological Chemistry 267, 17631-17634.
24. Russouw, P.S., Farrant, J.M., Brandt, W., Maeder, D. and Lindsey, G.G. (1995) Isolation and characterization of a heat-soluble protein from pea (Pisum sativum) embryos. Seed Science Research 5, 137-144.
25. Stacy, R.A.P., Espelund, M., Saeboe-Laisen, S., Hollung, K., Helliesen, E. and Jakobsen, K.S. (1995) Evolution of the Group 1 late ernbryogenesis abundant (Lea) genes: analysis of the Lea B19 gene family in barley. Plant Molecular Biology 28, 1039-1054.
26. Vertucci, C.W. and Farrant, J.M. (1995) Acquisition and loss of desiccation tolerance. pp 237-271 in Kigel, J. and Galili, G. (Eds). Seed development and germination. New York, Marcel Dekker Inc.