Protein synthesis-dependent and -independent mechanisms for the regulation of GnRH RNA transcript levels in GT1 cells

Brain Research

Volumn 752, Issue 1-2, 1997, Pages 294-300

  Yeo, Tracy T ^a,b   Gore, Andrea C ^a   Blum, Mariann ^a   Roberts, James L ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

actinomycin D; cycloheximide; DRB; GnRH; GT1 cell; phorbol ester

Indexed keywords

CYCLOHEXIMIDE; DACTINOMYCIN; GONADORELIN; MESSENGER RNA; PHORBOL ESTER;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RNA TRANSCRIPTION;

ANIMALS; CELL LINE; CYTOPLASM; GONADOTROPIN-RELEASING HORMONE; HYPOTHALAMUS; MACROMOLECULAR SUBSTANCES; NERVE TISSUE PROTEINS; NEURONS; NUCLEIC ACID SYNTHESIS INHIBITORS; PROTEIN SYNTHESIS INHIBITORS; RNA, MESSENGER; TETRADECANOYLPHORBOL ACETATE; TIME FACTORS; TRANSCRIPTION, GENETIC;

EID: 0030946038     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(96)01479-5     Document Type: Article

References (24)

1. Blum, M. and Roberts, J.L., Quantitation of nuclear low-level gene expression in central nervous system using solution hybridization and in situ hybridization, Meth. Neurosci., 1 (1989) 293-312.
2. Bruder, J.M., Krebs, W.D., Nett, T.M. and Wierman, M.E., Phorbol ester activation of the protein kinase C pathway inhibits gonadotropin-releasing hormone gene expression. Endocrinology, 131 (1992) 2552-2558.
3. Bruder, J.M. and Wierman, M.E., Evidence for transcriptional inhibition of GnRH gene expression by phorbol ester at a proximal promoter region, Mol. Cell. Endocrinol., 99 (1994) 177-182.
4. Cantley, L.C., Auger, K.R., Carpenter, C., Duckworth, B., Graziani, A., Kapeller, R. and Soltoff, S., Oncogenes and signal transduction, Cell, 64 (1991) 281-302.
5. Eraly, S.A. and Mellon, P.L., Regulation of gonadotropin-releasing hormone transcription by protein kinase C is mediated by evolutionarily conserved promoter-proximal elements. Mol. Endocrinol., 9 (1995) 848-859.
6. Gore, A.C., Ho, A. and Roberts, J.L., Translational efficiency of gonadotropin-releasing hormone messenger RNA is negatively regulated by phorbol ester in GT1-7 cells, Endocrinology, 136 (1995) 1620-1625.
7. Gore, A.C. and Roberts, J.L., Regulation of GnRH gene expression in vivo and in vitro, Front. Neuroendocrinol., in press.
8. Gore, A.C., Yeo, T.T., Ho, A. and Roberts, J.L., Post-transcriptional regulation of the gonadotropin-releasing hormone gene in GT1-7 cells, J. Neuroendocrinol., in press.
9. Harrold, S., Genovese, C., Kobrin, B., Morrison, S.L. and Milcarek, C., A comparison of apparent mRNA half-life using kinetic labeling techniques vs. decay following administration of transcriptional inhibitors, Ann. Biochem., 198 (1991) 19-29.
10. Jakubowski, M. and Roberts, J.L., Multiplex solution hybridization-ribonuclease protection assay for quantitation of different ribonucleic acid transcripts from snap-frozen neuroendocrine tissues of individual animals, J. Neuroendocrinol., 4 (1992) 79-89.
11. Lee, B.J., Kim, K. and Cho, W.K., Activation of intracellular pathways with forskolin and phorbol ester increases LHRH mRNA level in the rat hypothalamus superfused in vitro, Mol. Brain Res., 8 (1990) 185-191.
12. Liposits, Z., Merchenthaler, I., Wetsel, W.C., Reid, J.J., Mellon, P.L., Weiner, R.I. and Negro-Vilar, A., Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone, Endocrinology, 129 (1991) 1575-1583.
13. Mellon, P.L., Windle, J.J., Goldsmith, P.C., Padula, C.A., Roberts, J.L. and Weiner, R.I., Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis, Neuron, 5 (1990) 1-10.
14. Negro-Vilar, A., Conte, D. and Valenca, M., Transmembrane signals mediating neural peptide secretion: Role of protein kinase C activators and arachidonic acid metabolites in luteinizing hormone-releasing hormone secretion. Endocrinology, 119 (1986) 2796-2802.
15. Ojeda, S.R. and Negro-Vilar, A., Prostaglandin E2-induced luteinizing hormone-releasing hormone release involves mobilization of intracellular Ca2 +, Endocrinology, 116 (1985) 1763-1770.
16. Ojeda, S.R., Urbanski, H.F., Katz, K.H., Costa, M.E. and Conn, M., Activation of two different but complementary biochemical pathways stimulates release of hypothalamic luteinizing hormone-releasing hormone. Proc. Natl. Acad. Sci. USA, 83 (1986) 4932-4936.
17. Silverman, A.-J., The gonadotropin-releasing hormone (GnRH) neuronal system: Immunocytochemistry. In E. Knobil and J. Neill (Eds.), The Physiology of Reproduction, New York, Raven Press, 1988, pp. 1283-1304.
18. Weiner, R., Wetsel, W., Goldsmith, P., Martinez de la Escalera, G., Windle, J., Padula, C., Choi, C., Negro-Vilar, A. and Mellon, P., Gonadotropin-releasing hormone neuronal cell lines, Front. Neuroendocrinol., 13 (1992) 95-119.
19. Weiner, R.I., Findell, P.R. and Kordon, C., Role of classic and peptide neuromediators in the neuroendocrine regulation of LH and prolactin. In E. Knobil and J. Neill (Eds.), The Physiology of Reproduction, New York, Raven Press, 1988, pp. 1235-1281.
20. Wetsel, W.C., Immortalized hypothalamic luteinizing hormone-releasing hormone (LHRH) neurons: A new tool for dissecting the molecular and cellular basis of LHRH physiology, Cell. Mol. Neurobiol., 15 (1995) 43-78.
21. Wetsel, W.C., Eraly, S.A., Whyte, D.B. and Mellon, P.L., Regulation of gonadotropin-releasing hormone by protein kinase-A and -C in immortalized hypothalamic neurons. Endocrinology, 132 (1993) 2360-2370.
22. Yeo, T., Dong, K., Zeng, Z., Yu, K., Blum, M. and Roberts, J., Transcriptional and post-transcriptional regulation of gonadotropin-releasing hormone gene expression by the activation of protein kinase C pathway in a mouse hypothalamic GT1 cell line, Soc. Neurosci. Abstr., (1993) 618.
23. Yeo, T.T.S., Gore, A.C., Jakubowski, M., Dong, K., Blum, M. and Roberts, J.L., Characterization of gonadotropin-releasing hormone gene transcripts in a mouse hypothalamic neuronal GT1 cell line, Mol. Brain Res., in press.
24. Yu, K.-L., Yeo, T.T.S., Dong, K.-W., Jakubowski, M., Lackner-Arkin, C., Blum, M. and Roberts, J.L., Second messenger regulation of mouse gonadotrophin-releasing hormone gene expressin in immortalized mouse hypothalamic GT1-3 cells, Mol. Cell. Endocrinol., 102 (1994) 85-92.