Diminished heme oxygenase-1 mRNA expression in RPE cells from diabetic donors as quantitated by competitive RT/PCR

Current Eye Research

Volumn 16, Issue 4, 1997, Pages 380-386

  Da Silva, Jean Louis ^a   Stoltz, Robert A ^a   Dunn, Michael W ^a   Abraham, Nader G ^a   Shibahara, Shigaki ^b  

^a ROCKEFELLER UNIVERSITY   (United States)

^b TOHOKU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Competitive RT PCR; Diabetes mellitus; Heme oxygenase; Retinal pigment epithelium (RPE) cells

Indexed keywords

HEAT SHOCK PROTEIN; HEME OXYGENASE; MESSENGER RNA;

ARTICLE; CONTROLLED STUDY; DEFENSE MECHANISM; DIABETES MELLITUS; DIABETIC RETINOPATHY; HOMEOSTASIS; HUMAN; HUMAN CELL; HYPERTENSION; OXIDATIVE STRESS; PATHOPHYSIOLOGY; PIGMENT EPITHELIUM; PRIORITY JOURNAL; RETINA BLOOD VESSEL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0030943727     PISSN: 02713683     EISSN: None     Source Type: Journal    
DOI: 10.1076/ceyr.16.4.380.10695     Document Type: Article

References (36)

1. Engerman, R. L. (1989) Pathogenesis of diabetics retinopathy. Diabetes, 38, 1203-1206.
2. Stocker, R. (1990) Induction of haem oxygenase as a defence against oxidative stress. Free Radical Res. Commun. 9, 101-112.
3. Applegate, L. A., Luscher, P. and Tyrrell, R. M. (1991) Induction of heme oxygenase: a general response to oxidant stress in cultured mammalian cells. Cancer Res. 51, 974-978.
4. Abraham, N. G., da Silva, J.-L., Lavrovsky, Y., Stoltz, R. A., Kappas, A., Dunn, M. W. and Laniado Schwartzman, M. (1995) Adenovirus-mediated heme oxygenase-1 (HO-1) gene transfer into rabbit ocular tissues. Invest. Ophthalmol. Vis. Sci. 36, 2202-2210.
5. Kutty, R. K., Nagineni, C. N., Kutty, G., Hooks, J. J., Chader, G. J. and Wiggert, B. (1994) Increased expression of heme oxygenase-1 in human retinal pigment epithelial cells by transforming growth factor-b. J. Cell Physiol. 159, 371-378.
6. McCoubrey, W. K., Jr., Ewing, J. F. and Maines, M. D. (1992) Human heme oxygenase-2: characterization and expression of a full-length cDNA and evidence suggesting that the two HO-2 transcripts may differ by choice of polyadenylation signal. Arch. Biochem. Biophys. 295, 13-20.
7. Shibahara, S., Yoshizawa, M., Susuki, H., Takeda, K., Meguro, K. and Endo, K. (1993) Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation. J. Biochem. (Tokyo) 113, 214-218.
8. Krishnan Kutty, R., Kutty, G., Rodriguez, I. R., Chader, G. J. and Wiggert, B. (1994) Chromosomal localization of the human heme oxygenase genes: heme oxygenase-1 (HMOX1) maps to chromosome 22q12 and heme oxygenase-2 (HMOX2) maps to chromosome 16p13.3. Genomics, 20, 513-516.
9. Kuwano, A., Ikeda, H., Takeda, K., Nakai, H., Kondo, I. and Shibahara, S. (1994) Mapping of the human gene for inducible heme oxygenase to chromosome 22q12. Tohoku J. Exp. Med. 172 (4), 389-392.
10. Abraham, N. G., Lin, J. H.-C., Schwartzman, M. L., Levere, R. D. and Shibahara, S. (1988) The physiological significance of heme oxygenase. Int. J. Biochem. 20, 543-558.
11. Maines, M. D. (1992) Heme Oxygenase: Clinical Application and Functions. CRC Press, Boca Raton, FL, USA.
12. Shibahara, S., Muller, R. M. and Taguchi, H. (1987) Transcriptional control of rat heme oxygenase by heat shock. J. Biol. Chem. 262, 12889-12892.
13. Mitani, K., Fujita, H., Sassa, S. and Kappas, A. (1989) Heat shock induction of heme oxygenase mRNA in human Hep3B hepatoma cells. Biochem. Biophys. Res. Commun. 165, 437-441.
14. Keyse, S. M. and Tyrrell, R. M. (1989) Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite. Proc. Natl. Acad. Sci. USA, 86, 99-103.
15. Stocker, R., Yamamoto, Y., McDonagh, A. F., Glazer, A. N. and Ames, B. N. (1987) Bilirubin is an antioxidant of possible physiological importance. Science, 235, 1043-1046.
16. Vogt, B. A., Alam, J., Croatt, A. J., Vercellotti, G. M. and Nath, K. A. (1995) Acquired resistance to acute oxidative stress: possible role of heme oxygenase and ferritin. Lab. Invest. 72, 474-483.
17. Llesuy, S. F. and Tomaro, M. L. (1994) Heme oxygenase and oxidative stress: evidence of involvement of bilirubin as physiological protector against oxidative damage. Biochim. Biophys. Acta, 1223, 9-14.
18. Nath, K. A., Balla, G., Vercellotti, G. M., Balla, J., Jacob, H. S., Levitt, M. D. and Rosenberg, M. E. (1992) Induction of heme oxygenase is a rapid, protective response in rhabdomyolysis in the rat. J. Clin. Invest. 90, 267-270.
19. Kutty, G., Hayden, B., Osawa, Y., Wiggert, B., Chader, G. J. and Kutty, R. K. (1992) Heme oxygenase: expression in human retina and modulation by stress agents in a human retinoblastoma cell model system. Curr. Eye Res. 11, 153-160.
20. Abraham, N. G., Lavrovsky, Y., Laniado Schwartzman, M., Stoltz, R. A., Gerritsen, M. E. and Kappas, A. (1995) Transfection of human heme oxygenase gene into rabbit endothelial cells and possible protection against hemoglobin toxicity. Proc. Natl. Acad. Sci. USA, 92, 6798-6802.
21. Vile, G. F., Basu-Modak, S., Waltner, C. and Tyrrell, R. M. (1994) Heme oxygenase-1 mediates an adaptive response to oxidative stress in human skin fibroblast. Proc. Natl. Acad. Sci. USA, 91, 2607-2610.
22. Krishnan Kutty, R., Kutty, G., Wiggert, B., Chader, G. J., Darrow, R. M. and Organisciak, D. T. (1995) Induction of heme oxygenase-1 in the retina by intense visible light: suppression by the antioxidant dimethylthiourea. Proc. Natl. Acad. Sci. USA, 92, 1177-1181.
23. Cross, N. C. (1995) Quantitative PCR techniques and applications. Br. J. Haematol. 89, 693-697.
24. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidium. Anal. Biochem. 162, 156-159.
25. Kigasawa, K., Soushi, S., Tanaka, Y. and Obazawa, H. (1994) Morphologic and chromosomal study of a human retinal pigment epithelial cell line. Jpn. J. Ophthalmol. 38, 10-15.
26. Becker-Andre, M. and Hahlbrock, K. (1989) Absolute mRNA quantification using the polymerase chain reaction (PCR): a novel approach by a PCR aided transcript titration assay (PATTY). Nucleic Acids Res. 17, 9437-9446.
27. Wang, A. M., Doyle, M. V. and Mark, D. F. (1989) Quantitation of mRNA by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA, 86, 9717-9721.
28. Gilliland, G., Perrin, S., Blanchard, K. and Bunn, H. F. (1990) Analysis of cytokines mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc. Natl. Acad. Sci. USA, 87, 2725-2729.
29. Cross, N. C., Feng, L., Chase, A., Bungey, J., Hughes, T. P. and Goldman, J. M. (1993) Competitive polymerase chain reaction to estimate the number of BCR-ABL transcripts in chronic myeloid leukemia patients after bone marrow transplantation. Blood, 82, 1929-1936.
30. Frenoy, N., Chabli, A., Sol, O., Goldschmit, E., Lemonnier, M. P., Misset, J. L. and Debuire, B. (1994) Application of a new protocol for nested PCR to the detection of minimal residual bcr/abl transcripts. Leukemia, 8, 1411-1414.
31. Radich, J. P., Gehly, G., Gooley, T., Bryant, E., Clift, R. A., Collins, S., Edmands, S., Kirk, J., Lee, A., Kessler, P., Schoch, G., Buckner, C. D., Sullivan, K. M., Appelbaum, F. R. and Thomas, E. G. (1995) Polymerase chain reaction detection of the BCR-ABL fusion transcript after allogeneic marrow transplantation for chronic myeloid leukemia: results and implications in 346 pati. Blood, 85, 2632-2638.
32. Lion, T., Gaiger, A., Henn, T., Horth, E., Haas, O. A., Geissler, K. and Gadner, H. (1995) Use of quantitative polymerase chain reaction to monitor residual disease in chronic myelogenous leukemia during treatment with interferon. Leukemia, 9, 1353-1360.
33. Krishnan Kutty, R., Kutty, G., Nagineni, C. N., Hooks, J. J., Chader, G. J. and Wiggert, B. (1994) RT-PCR assay for heme oxygenase-1 and heme oxygenase-2: a sensitive method to estimate cellular oxidative damage. Ann. NY Acad. Sci. 738, 427-430.
34. Ahmed, A. H., Notarianni, L. J. and Jones, R. W. (1996) A competitive single-step reverse transcriptase-polymerase chain reaction assay for quantification of heme oxygenase 1. Analyt. Biochem. 237, 149-152.
35. Yan, S. D., Schmidt, A. M., Anderson, G. M., Zhang, J., Brett, J., Zou, Y S., Pinsky, D. and Stern, D. (1994) Enhanced cellular oxidant stress by the interaction of advanced glycation end products with their receptors/binding proteins. J. Biol. Chem. 269 (13), 9889-9897.
36. Bitar, M. and Weiner, M. (1984) Diabetes-induced metabolic alterations in heme synthesis and degradation and various heme-containing enzymes in female rats. Diabetes, 33 (1), 37-44.