Heterologous expression of biphenyl dioxygenase-encoding genes from a gram-positive broad-spectrum polychlorinated biphenyl degrader and characterization of chlorobiphenyl oxidation by the gene products

Journal of Bacteriology

Volumn 179, Issue 6, 1997, Pages 1924-1930

  McKay, David B ^a,b   Seeger, Michael ^a,c   Zielinski, Marco ^a   Hofer, Bernd ^a   Timmis, Kenneth N ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b UNIVERSITY OF NEW ENGLAND   (Australia)

^c UNIVERSITY OF CHILE   (Chile)

Author keywords

[No Author keywords available]

Indexed keywords

BIPHENYLBIOXYGENASE; OXYGENASE; POLYCHLORINATED BIPHENYL; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; BIODEGRADATION; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GRAM POSITIVE BACTERIUM; MESSENGER RNA SYNTHESIS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA;

EID: 0030941678     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.6.1924-1930.1997     Document Type: Article

References (38)

1. Abramowicz, D. A. 1990. Aerobic and anaerobic biodegradation of PCBs: a review. Crit. Rev. Biotechnol. 10:241-251.
2. Ahmad, D., M. Sylvestre, and M. Sondossi. 1991. Subcloning of bph genes from Pseudomonas testosteroni B-356 in Pseudomonas putida and Escherichia coli: evidence for dehalogenation during initial attack on chlorobiphenyls. Appl. Environ. Microbiol. 57:2880-2887.
3. Asturias, J. A., E. Diaz, and K. N. Timmis. 1995. The evolutionary relationship of biphenyl dioxygenase from Gram-positive Rhodococcus globerutus P6 to multicomponent dioxygenases from Gram-negative bacteria. Gene 156:11-18.
4. Asturias, J. A., L. D. Eltis, M. Prucha, and K. N. Timmis. 1994. Analysis of three 2,3-dihydroxybiphenyl 1,2-dioxygenases found in Rhodococcus globeruins P6. Identification of a new family of extradiol dioxygenases. J. Biol. Chem. 269:7807-7815.
5. Asturias, J. A., E. Moore, M. M. Yakimov, S. Klatte, and K. N. Timmis. 1994. Reclassification of the polychlorinated biphenyl-degraders Acinefobacter sp. strain P6 and Corynebacterium sp. strain MB1 as Rhodococcus globerulus. Syst. Appl. Microbiol. 17:226-231.
6. Asturias, J. A., and K. N. Timmis. 1993. Three different 2,3-dihydroxybiphenyl-1,2-dioxygenase genes in the gram-positive polychlorobiphenyl-degrading bacterium Rhodococcus globerulus P6. J. Bacteriol. 175:4631-4640.
7. Bedard, D. L., R. E. Wagner, M. J. Brennan, M. L. Haberl, and J. F. Brown, Jr. 1987. Extensive degradation of Aroclors and environmentally transformed polychlorinated biphenyls by Alcaligenes eutrophus H850. Appl. Environ. Microbiol. 53:1094-1102.
8. Boyle, A. W., C. J. Silvin, J. P. Hassett, J. P. Nakas, and S. W. Tanenbaum. 1992. Bacterial PCB biodegradation. Biodegradation 3:285-298.
9. de Smit, M. H., and J. van Duin. 1994. Translational initiation on structured messengers. J. Mol. Biol. 235:173-184.
10. Denome, S. A., E. S. Olson, and K. D. Young. 1993. Identification and cloning of genes involved in specific desulfurization of dibenzothiophene by Rhodococcus sp. strain IGTS8. Appl. Environ. Microbiol. 59:2837-2843.
11. Erickson, B. D., and F. J. Mondello. 1992. Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400. J. Bacteriol. 174:2903-2912.
12. Franklin, F. C. H., M. Bagdasarian, M. M. Bagdasarian, and K. N. Timmis. 1983. Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway. Proc. Natl. Acad. Sci. USA 78:7458-7462.
13. Furukawa, K., F. Matsumura, and K. Tonomura. 1978. Alcaligenes and Acinetobacter strains capable of degrading polychlorinated biphenyls. Agric. Biol. Chem. 42:543-548.
14. Furukawa, K., and T. Miyazaki. 1986. Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes. J. Bacteriol. 166:392-398.
15. Furukawa, K., and H. Suzuki. 1988. Gene manipulation of catabolic activities for production of intermediates of various biphenyl compounds. Appl. Microbiol. Biotechnol. 29:363-369.
16. Furukawa, K., N. Tomizuka, and A. Kamibayashi. 1979. Effect of chlorine substitution on the bacterial metabolism of various polychlorinated biphenyls. Appl. Environ. Microbiol. 38:301-310.
17. Furukawa, K., K. Tonomura, and A. Kamibayashi. 1978. Effect of chlorine substitution on the biodegradability of polychlorinated biphenyls. Appl. Environ. Microbiol. 35:223-227.
18. Furukawa, K., K. Tonomura, and A. Kamibayashi. 1979. Metabolism of 2,4,4′-trichlorobiphenyl by Acinetobacter sp. P6. Agric. Biol. Chem. 43:1577-1583.
19. Gualerzi, C. O., and C. L. Pon. 1990. Initiation of mRNA translation in prokaryotes. Biochemistry 29:5881-5888.
20. Haddock, J. D., J. R. Horton, and D. T. Gibson. 1995. Dihydroxylation and dechlorination of chlorinated biphenyls by purified biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400. J. Bacteriol. 177:20-26.
21. Hansen, L. G. 1987. Environmental toxicology of polychlorinated biphenyls. Environ. Toxin Ser. 1:15-48.
22. Herrero, M., V. de Lorenzo, and K. N. Timmis. 1990. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J. Bacteriol. 172:6557-6567.
23. Hofer, B., R. Blasco, M. Megharaj, M. Seeger, D. McKay, R.-M. Wittich, D. H. Pieper, and K. N. Timmis. 1996. Pseudomonads designed for bioremediation and circumvention of undesirable routes, 121-131. In T. Nakazawa, K. Furukawa, D. Haas, and S. Silver (ed.), Molecular biology of pseudomonads. American Society for Microbiology, Washington, D.C.
24. Hofer, B., L. D. Eltis, D. N. Dowling, and K. N. Timmis. 1993. Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation. Gene 130:47-55.
25. Kirsch, N. H., and H.-J. Stan. 1994. Gas chromatographic-mass spectrometric determination of chlorinated cis-1,2-dihydroxycyclohexadienes and chlorocatechols as their boronates. J. Chromatogr. A 684:277-287.
26. Kovach, M. E., R. W. Phillips, P. H. Elzer, R. M. Roop II, and K. M. Peterson. 1994. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 16:800-802.
27. Looman, A. C., J. Bodlaender, M. de Gruyter, A. Vogelaar, and P. H. van Knippenberg. 1986. Secondary structure as primary determinant of the efficiency of ribosomal binding sites in Escherichia coli. Nucleic Acids Res. 14:5481-5497.
28. Masai, E., A. Yamada, J. M. Healy, T. Hatta, K. Kimbara, M. Fukuda, and K. Yano. 1995. Characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol. 61:2079-2085.
29. Mason, J. R., and R. Cammack. 1992. The electron-transport proteins of hydroxylating bacterial dioxygenases. Annu. Rev. Microbiol. 46:277-305.
30. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
31. Seeger, M., K. N. Timmis, and B. Hofer. Unpublished results.
32. Seeger, M., K. N. Timmis, and B. Hofer. 1995. Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Pseudomonas sp. strain LB400. Appl. Environ. Microbiol. 61:761-768.
33. Seeger, M., K. N. Timmis, and B. Hofer. 1995. Degradation of chlorobiphenyls catalyzed by the bph-encoded biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase of Pseudomonas sp. LB400. FEMS Microbiol. Lett. 133:259-264.
34. Shao, Z. Q., W. Seffens, W. Mulbry, and R. M. Behki. 1995. Cloning and expression of the s-triazine hydrolase gene (trzA) from Rhodococcus corallinus and development of Rhodococcus recombinant strains capable of dealkylating and dechlorinating the herbicide atrazine. J. Bacteriol. 177:5748-5755.
35. Silberhorn, E. M., H. P. Glauert, and L. W. Robertson. 1990. Carcinogenicity of polyhalogenated biphenyls: PCBs and PBBs. Crit. Rev. Toxicol. 20:439-496.
36. Studier, F. W. 1991. Use of bactcriophage T7 lysozyme to improve an inducihle T7 expression system. J. Mol. Biol. 219:37-44.
37. Studier, F. W., and B. A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
38. Wang, Y., J. Garnon, D. Labbé, H. Bergeron, and P. C. K. Lau. 1995. Sequence and expression of the bpdC1C2BADE genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by Rhodococcus sp. M5. Gene 164:117-122.