Nonradioactive PCR-SSCP assay to detect apoB Arg3500 to Gln mutation using a minigel format

BioTechniques

Volumn 22, Issue 3, 1997, Pages 446-447

  Hennessy, Lori K ^a,c   Pullinger, Clive R ^b  

^a Amersham Biosciences   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c Hoefer Pharmaeia Biotech   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BINDING AFFINITY; GENETIC SCREENING; GENETIC SUSCEPTIBILITY; HUMAN; ISCHEMIC HEART DISEASE; NUCLEIC ACID BASE SUBSTITUTION; POINT MUTATION; POLYMERASE CHAIN REACTION; PROTEIN LIPID INTERACTION; SEQUENCE ANALYSIS; SINGLE STRAND CONFORMATION POLYMORPHISM;

EID: 0030941134     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97223bm15     Document Type: Article

References (6)

1. Hansen, P.S., N. Rudiger, A. Tybjaerg-Hansen, O. Faergeman and N. Gregersen. 1991. Detection of the apoB-3500 mutation (glutamine for arginine) by gene amplification and cleavage with MspI. J. Lipid Res. 32:1229-1233.
2. Inneraity, T.L., R.W. Mahley, K.H. Weisgraber, T.P. Bersot, R.M. Krauss, G.L. Vega, S.M. Grundy, W. Friedl et al. 1990. Familial defective apolipoprotein B-100; a mutation of apolipoprotein B that causes hypercholesterolemia. J. Lipid Res. 31:1337-1349.
3. Orita, M., S. Youichi, T. Sekiya and K. Hayashi. 1989. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5:874-879.
4. Pullinger, C.R., L.K. Hennessy, J.E. Chatteron, W. Liu, J.A. Love, C.M. Mendel, P.H. Frost, M.J. Malloy et al. 1995. Familial ligand-defective apolipoprotein B. Identification of a new mutation that decreases LDL receptor binding affinity. J. Clin. Invest. 95:1225-1234.
5. Tybjaerg-Hansen, A. , J. Gallagher, J. Vincent, R. Houlston, P. Talmud, A.M. Dunning, M. Seed, A. Hamsten et al. 1990. Familial defective apolipoprotein B-100: detection in the United Kingdom and Scandinavia, and clinical characteristics of ten cases. Athersclerosis 80:235-242.
6. Vidal-Puig, A. and D.E. Moller. 1994. Comparative sensitivity of alternative single-strand conformation polymorphism (SSCP) methods. BioTechniques 17:490-496.