Cloning and characterization of a cDNA encoding Xenopus laevis αo1 isoform of the Go protein

Gene

Volumn 188, Issue 1, 1997, Pages 137-141

  Paquereau, Laurent ^a   Devic, Eric ^a   Audigier, Yves ^a  

^a UNIVERSITÉ PAUL SABATIER   (France)

Author keywords

Alternative splicing; Early embryogenesis; Heterotrimeric GTP binding proteins; Recombinant DNA

Indexed keywords

COMPLEMENTARY DNA; GUANINE NUCLEOTIDE BINDING PROTEIN; ISOPROTEIN; PROTEIN SUBUNIT; REPETITIVE DNA;

ALPHA CHAIN; ARTICLE; CARBOXY TERMINAL SEQUENCE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; XENOPUS LAEVIS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE EXPRESSION; GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; GTP-BINDING PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN BIOSYNTHESIS; RNA, MESSENGER; XENOPUS LAEVIS;

MAMMALIA; XENOPUS LAEVIS;

EID: 0030941042     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00799-8     Document Type: Article

References (24)

1. Bertrand, P., Sanford, J., Rudolph, U., Codina, J. and Birnbaumer, L. (1990) At least three alternatively spliced mRNAs encoding two a subunits of the Go GTP-binding protein can be expressed in a single tissue. J. Biol. Chem. 265, 18576-18580.
2. Bourne, H.R., Sanders, D.A. and McCormick, F. (1991) The GTPase superfamily: conserved structure and molecular mechanism. Nature 349, 117-126.
3. Cali, J.J., Balcueva, E.A., Rybalkin, I. and Robishaw, J.D. (1992) Selective tissue distribution of G protein γ subunits, including a new form of the γ subunits identified by cDNA cloning. J. Biol. Chem. 267, 24023-24027.
4. Conklin, B.R., Farfel, Z., Lustig, K.D., Julius, D. and Bourne, H.R. (1993) Substitution of three amino acids switches receptor specificty of Gqα to that of Giα. Nature 363, 274-276.
5. de Sousa, S.M., Hoveland, L.L., Yarfitz, S. and Hurley, J.B. (1989) The Drosophila Goα-like protein gene produces multiple transcripts and is expressed in the nervous system and in ovaries. J. Biol. Chem. 264, 18544-18551.
6. Fung, B.K.-K. and Nash, C.R. (1983) Characterization of transducin from bovine retinal rod outer segments. II. Evidence for distinct binding sites and conformational changes revealed by limited proteolysis with trypsin. J. Biol. Chem. 258, 10503-10510.
7. Gautam, N., Northup, J., Tamir, H. and Simon, M.I. (1990) G protein diversity is increased by associations with a variety of γ subunits. Proc. Natl. Acad. Sci. USA 87, 7973-7977.
8. Gilman, A.G. (1987) G proteins: transducers of receptor-generated signals. Annu. Rev. Biochem. 56, 615-649.
9. Hsu, W.H., Rudolph, U., Sanford, J., Bertrand, P., Olate, J., Nelson, C., Moss, L.G., Boyd, A.E., Codina, J. and Birnbaumer, L. (1990) Molecular cloning of a novel splice variant of the a subunit of the mammalian Go protein. J. Biol. Chem. 265, 11220-11226.
10. Journot, L., Pantaloni, C., Bockaert, J., and Audigier, Y. (1991) Deletion within the amino-terminal region of Gsα impairs its ability to interact with βγ subunits and to activate adenylylcyclase. J. Biol. Chem. 266, 9009-9015.
11. Kleuss, C., Hescheler, J., Ewel, C., Rosenthal, W., Schultz, G. and Wittig, B. (1991) Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents. Nature 353, 43-48.
12. Kleuss, C., Scherübl, H., Hescheler, J., Schultz, G. and Wittig, B. (1992) Different β subunits determine G-protein interaction with transmembrane receptors. Nature 358, 424-426.
13. Kleuss, C., Scherübl, H., Hescheler, J., Schultz, G. and Wittig, B. (1993) Selectivity in signal transduction determined by γ subunits of heterotrimeric G proteins. Science 259, 832-834.
14. Krieg, P.A. and Melton, D.A. (1985) Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs. EMBO J. 4, 3463-3471.
15. Neer, E.J. (1995) Heterotrimeric G proteins: organizers of transmembrane signals. Cell 80, 249-257.
16. Newport, J. and Kirschner, M. (1982) A major developmental transition in early Xenopus embryos. I. Characterization and timing of cellular changes at the midblastula stage. Cell. 30, 675-686.
17. Nieuwkoop, P. and Faber, J. (1967) Normal Table of Xenopus laevis (Daudin), 2nd ed. North Holland Publ. Co., Amsterdam.
18. Olate, J., Jorquera, H., Purcell, P., Codina, J., Birnbaumer, L. and Allende, J.E. (1989) Molecular cloning and sequence determination of a cDNA coding for the α sub unit of a Go-type protein of Xenopus laevis oocytes. FEBS Lett. 244, 188-192.
19. Oñate, A., Herrera, L., Antonelli, M., Birnbaumer, L. and Olate, J. (1992) Xenopus laevis oocyte Got subunits mRNAs. Detection and quantitation during oogenesis and early embryogenesis by competitive reverse PCR. FEBS Lett. 313, 213-219.
20. Simon, M.I., Strathmann, M.P. and Gautam, N. (1991) Diversity of G proteins in signal transduction. Science 252, 802-808.
21. Strathmann, M.P., Wilkie, T.M. and Simon, M.I. (1990) Alternative splicing produces transcripts encoding two forms of the α subunit of GTP-binding protein Go. Proc. Natl. Acad. Sci. USA 87, 6477-6481.
22. Sullivan, K.A., Miller, R.T., Masters, S.B., Beiderman, B., Heideman, W. and Bourne, H.R. (1987) Identification of receptor contact site involved in receptor-G protein coupling. Nature 330, 758-760.
23. Watson, A. J., Katz, A. and Simon, M.I. (1994) A fifth member of the mammalian G-protein β-subunit family. J. Biol. Chem. 269, 22150-22156.
24. Yoon, J., Shortridge, R.D., Bloomquist, B.T., Schneuly, S., Perdew, M.H. and Pak, W.L. (1989) Molecular characterization of Drosophila gene encoding Goα subunit homolog. J. Biol. Chem. 264, 18536-18543.