Overexpression of BsoBI restriction endonuclease in E. coli, purification of the recombinant BsoBI, and identification of catalytic residues of BsoBI by random mutagenesis

Gene

Volumn 188, Issue 1, 1997, Pages 35-39

  Ruan, Hong ^a   Lunnen, Keith D ^a   Pelletier, John J ^a   Xu, Shuang Yong ^a  

^a NEW ENGLAND BIOLABS   (United States)

Author keywords

AvaI isoschizomer; Binding proficient and cleavage deficient mutants; Hydroxylamine mutagenesis; PCR mutagenesis; Restriction enzyme

Indexed keywords

BACTERIAL ENZYME; HYDROXYLAMINE; METAL ION; METHYLTRANSFERASE; PLASMID VECTOR; RECOMBINANT ENZYME; RESTRICTION ENDONUCLEASE;

ALLELE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM TRANSFORMATION; CONTROLLED STUDY; DNA SEQUENCE; ELECTROPHORESIS; ELECTROPHORETIC MOBILITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE INSERTION; GENE MUTATION; GENE OVEREXPRESSION; GEOBACILLUS STEAROTHERMOPHILUS; MUTAGENESIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DNA BINDING;

AMINO ACID SEQUENCE; BACILLUS STEAROTHERMOPHILUS; BINDING SITES; CATALYSIS; CLONING, MOLECULAR; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MUTAGENESIS; RECOMBINANT FUSION PROTEINS; VARIATION (GENETICS);

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS;

EID: 0030941040     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00773-1     Document Type: Article

References (21)

1. Athanasiadis, A., Blassi, M., Kotsifaki, D., Tucker, P.A., Wilson, K.S. and Kokkinidis, M. (1994) Crystal structure of PvuII endonuclease reveals extensive structural homologies to EcoRV. Struct. Biol. 1, 469-475.
2. Bickle, T.A. and Kruger, D.H. (1993) Biology of DNA restriction. Microbiol. Rev. 57, 434-450.
3. Cheng, X., Balendiran, K., Schildkraut, I. and Anderson, J.E. (1994) Structure of PuvII endonuclease with cognate DNA. EMBO J. 13, 3927-3935.
4. Heitman, J. (1993) On the origins, structures and functions of restriction-modification enzymes. Plenum Press, New York.
5. Kim, Y.C., Grable, J.C., Love, R., Greene, P.J. and Rosenberg, J.M. (1990) Refinement of EcoRI endonuclease crystal structure: a revised protein chain tracing. Science 249, 1307-1309.
6. King, K., Benkovic, S.J. and Modrich, P. (1989) Glu-111 is required for activation of the DNA cleavage center of EcoRI endonuclease. J. Biol. Chem. 264, 11807-11815.
7. 2+ binding to the active site of EcoRV endonuclease: a crystallographic study of complexes with substrate and product DNA at 2 A resolution. Biochemistry 34, 683-696.
8. Newman, M., Strzelecka, T., Dorner, L.F., Schildkraut, I. and Aggarwal, A.K. (1994) Structure of restriction endonuclease BamHI and its relationship to EcoRI. Nature 368, 660-664.
9. Newman, M., Strzelecka, T., Dorner, L.F., Schildkraut, I. and Aggarwal, A.K. (1995) Structure of BamHI endonuclease bound to DNA: partial folding and unfolding on DNA binding. Science 269, 656-663.
10. Posfai, J., Bhagwat, A.S., Posfai, G. and Roberts, R.J. (1989) Predictive motifs derived from cytosine methyltransferases. Nucleic Acids Res. 17, 2421-2435.
11. Roberts, R.J. and Halford, S.E. (1993) Type II restriction endonucleases. Cold Spring Harbor Press, Cold Spring Harbor, NY.
12. Roberts, R.J. and Macelis, D. (1996) REBASE - restriction enzymes and methylases. Nucleic Acids Res. 24, 223-235.
13. Ruan, H., Lunnen, K., Scott, M., Moran, L.S., Slatko, B.E., Pelletier, J., Hess, E.J., Benner, J.I., Wilson, G.G. and Xu, S.-y. (1996) Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems. Mol. Gen. Genet. 252, 695-699.
14. Selent, U., Ruter, T., Kohler, E., Liedtke, M., Thielking, V., Alves, J., Oelgeschlager, T., Wolfes, H., Peters, F. and Pingoud, A. (1992) A site-directed mutagenesis study to identify amino acid residues involved in the catalytic function of the restriction endonuclease EcoRV. Biochemistry 31, 4808-4815.
15. Skoglund, C.M., Smith, H.O. and Chandrasegeran, S. (1990) Construction of an efficient overproducer clone of HinfI restriction endonuclease using the polymerase chain reaction. Gene 88, 1-5.
16. Thielking, V., Selent, U., Kohler, E., Wolfes, H., Pieper, U., Geiger, R., Urbanke, C., Winkler, F.K. and Pingoud, A. (1991) Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis. Biochemistry 30, 6416-6422.
17. Wilson, G. (1991) Organization of restriction-modification systems. Nucleic Acids Res. 19, 2539-2566.
18. Wilson, G.G. and Murray, N.E. (1991) Restriction and modification systems. Annu. Rev. Genet. 25, 585-627.
19. Winkler, F.K., Banner, D.W., Tsernoglou, D., Brown, R.S., Heathman, S.P., Bryan, R.K., Martin, P.D., Petratos, K. and Wilson, K.S. (1993) The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments. EMBO J. 12, 1781-1795.
20. Withers, B.E. and Dunbar, J.C. (1995) DNA determinants in sequence-specific recognition by XmaI endonuclease. Nucleic Acids Res. 23, 3571-3577.
21. Xu, S.-y. and Schildkraut, I. (1991) Isolation of BamHI variants with reduced cleavage activities. J. Biol. Chem. 206, 4425-4429.