Identification of a 37 kDa Tacrolimus, Sirolimus and cyclosporine binding immunophilin possessing glyceraldehyde 3-phosphate dehydrogenase activity isolated from the JURKAT T cell line

Clinical Biochemistry

Volumn 30, Issue 2, 1997, Pages 129-133

  Murthy, Jayasimha N ^a   Goodyear, Nancy ^a,b   Soldin, Steven J ^a,b,c  

^a GEORGE WASHINGTON UNIVERSITY   (United States)

^b CATHOLIC UNIVERSITY OF AMERICA   (United States)

^c CHILDREN'S NATIONAL MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; IMMUNOPHILIN; RAPAMYCIN; TACROLIMUS;

AMINO ACID COMPOSITION; ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; HUMAN; HUMAN CELL; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC FOCUSING; LEUKEMIA CELL LINE; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION; T LYMPHOCYTE ACTIVATION;

ADJUVANTS, IMMUNOLOGIC; AMINO ACID SEQUENCE; CARRIER PROTEINS; CHROMATOGRAPHY, ION EXCHANGE; CYCLOSPORINE; DNA GLYCOSYLASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; JURKAT CELLS; MOLECULAR SEQUENCE DATA; N-GLYCOSYL HYDROLASES; POLYENES; SIROLIMUS; TACROLIMUS; URACIL-DNA GLYCOSIDASE;

EID: 0030940628     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-9120(96)00160-9     Document Type: Article

References (12)

1. Kino T, Hatanaka H, Miyata S, et al. FK-506, a novel immunosuppressant isolated from a Streptomyces II. Immunosuppressive effect of FK-506 in vitro. J Antibiot 1987; 40: 1249-56.
2. Bierer BE. Cyclosporine A, FK506 and rapamycin: binding to immunophilins and biological action. Chem Immunol 1994; 59: 128-55.
3. Dumont FJ, Melino MR, Starucil MJ, Koprak SL, Fischer PA, Sigal NH. The immunosuppressive macrolides FK-506 and rapamycin act as reciprocal antagonists in murine T cells. J Immunol 1990; 144: 1418-24.
4. Thomson AW, Bonham A, Zeevi A. Mode of action of Tacrolimus (FK506): Molecular and cellular mechanism. Ther Drug Monit 1995; 17: 584-91.
5. Sabatini DM, Erdjument-Bromage H, Lui M, et al. RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and homologus to yeast TORs. Cell 1994; 78: 35-43.
6. Murthy JN, Chen Y, Soldin SJ. Identification of a 14 kDa FK-506/rapamycin binding immunophilin from calf thymus. Clin Biochem 1994; 27: 357-365.
7. Bergmeyer HU, Gawehn K, Grassl M. Enzymatic assay of glyceraldehyde 3-phosphate dehydrogenase. In: Bergmeyer, HU, Ed. Methods of enzymatic analysis. 2nd ed., Vol 1. Pp. 466-67. New York: Academic press, 1974.
8. Yem AM, Tomasselli AG, Heinrikson RL, et al. The Hsp 567 component of steroid receptor complexes binds to immobilized FK506 and shows homology to FKBP12 and FKBP13. J Biol Chem 1992; 267: 2868-71.
9. Tai PK, Albers MW, Chang H, Faber LE, Schreiber SL. Association of a 59kDa immunophilin with glucocorticoid receptor complex. Science 1992; 256: 1315-18.
10. Donnelly JG, Soldin SJ. Partial characterization of a 52 kDa CsA/FK506/rapamycin binding protein. Clin Biochem 1994; 27: 367-72.
11. Mayer-Siegler K, Mauro DJ, Seal G, Wurzer J, DeRiel JK, Sirover MA. A human nuclear uracil DNA glycosylase is the 37 kDa subunit of glyceraldehyde 3-phosphate dehydrogenase. Proc Natl Acad Sci USA 1991; 88: 8460-4.
12. Soldin SJ. Receptor (immunophilin-binding) assays for immunosuppressive drugs. Ther Drug Monit 1995; 17: 574-76.