Metabolic and permeability changes caused by thiamine deficiency in immortalized rat brain microvessel endothelial cells

Brain Research

Volumn 756, Issue 1-2, 1997, Pages 133-140

  Romero, I A ^a   Rist, R J ^a   Aleshaiker, A ^a   Abbott, N J ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

Brain endothelium; Cultured monolayer; Cytoskeleton; Permeability; Pyrithiamine

Indexed keywords

F ACTIN; FLUORESCEIN ISOTHIOCYANATE DEXTRAN; LACTIC ACID; PYRITHIAMINE; SUCROSE; THIAMINE;

ANIMAL CELL; ARTICLE; BLOOD BRAIN BARRIER; BRAIN; CELL IMMORTALIZATION; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; CYTOTOXICITY; GLUCOSE INTAKE; NONHUMAN; PRIORITY JOURNAL; RAT; THIAMINE DEFICIENCY; VASCULAR ENDOTHELIUM;

ACTINS; ANIMALS; ANTIMETABOLITES; BLOOD VESSELS; CAPILLARY PERMEABILITY; CELL LINE, TRANSFORMED; CYTOSKELETON; ENDOTHELIUM, VASCULAR; GLUCOSE; LACTIC ACID; MICROCIRCULATION; OXIDATION-REDUCTION; PYRITHIAMINE; RATS; TETRAZOLIUM SALTS; THIAMINE DEFICIENCY; THIAZOLES;

EID: 0030940277     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(97)00127-3     Document Type: Article

References (44)

1. Abbott, N.J., Couraud, P.-O., Roux, F. and Begley, D.J., Studies on an immortalized brain endothelial cell line: characterisation, permeability and transport. In: New Concepts of a Blood - Brain Barrier, Eds. J. Greenwood, D.J. Begley, M.B. Segal and S. Lightman. Plenum Press, New York (1995) pp. 239-249.
2. Begley, D.J., Lechardeur, D., Chen, Z.D., Rollinson, C., Bardoul, M., Roux, F., Scherman, D. and Abbott, N.J., Functional expression of p-glycoprotein in an immortalised cell line of rat brain endothelial cells, RBE4, J. Neurochem., 67 (1996) 988-995.
3. Bergmeyer, H.U., Bernt, E., Schmidt, F. and Stock, H., In: Methods of Enzymatic Analysis, Vol. 3. Bergmeyer, H.U. (Ed.), Academic Press Inc., New York and London (1974) pp. 1196-1201.
4. Bettendorff, L., Goessens, G., Sluse, F., Wins, P., Bureau, M., Laschet, J. and Grisar, T., Thiamine deficiency in cultured neuroblastoma cells: effect on mitochondrial function and peripheral benzodiazepine receptors, J. Neurochem., 64 (1995) 2013-2021.
5. Bettendorff, L., Sluse, F., Goessens, G., Wins, P. and Grisar, T., Thiamine deficiency-induced partial necrosis and mitochondrial uncoupling in neuroblastoma cells are rapidly reversed by addition of thiamine, J. Neurochem., 65 (1995) 2178-2184.
6. Butterworth, R.F., Effects of thiamine deficiency on brain metabolism: implications for the pathogenesis of the Wernicke-Korsakoff syndrome, Alcohol Alcoholism, 24 (1989) 271-279.
7. Calingasan, N.Y., Baker, H., Sheu, K.-F.R. and Gibson, G.E., Blood-brain barrier abnormalities in vulnerable brain regions during thiamine deficiency, Exp. Neurol., 134 (1995) 64-72.
8. Cavanagh, J.B., Selective vulnerability in acute energy deprivation syndromes, Neuropathol. Appl. Neurobiol., 19 (1993) 461-470.
9. Dejana, E., Corada, M. and Lampugnani, M.G., Endothelial cell-to-cell junctions, FASEB J., 9 (1995) 910-918.
10. Dehouck, M.-P., Méresse, S., Delorme, P., Fruchart, J.-C. and Cecchelli, R., An easier, reproducible, and mass-production method to study the blood-brain barrier in vitro, J. Neurochem., 54 (1990) 1798-1801.
11. Durieu-Trautmann, O., Federici, C., Creminon, C., Foignant-Chaverot, N., Roux, F., Claire, M., Strosberg, A.D. and Couraud, P.O., Nitric oxide and endothelin secretion by brain microvessel endothelial cells: regulation by cyclic nucleotides, J. Cell. Physiol., 155 (1993) 104-111.
12. Finglas, P.M., Thiamin, Int. J. Vitamin Nutrition Res., 63 (1993) 270-274.
13. Goldblum, S.E., Ding, X., Brann, T.W. and Campbell-Washington, J., Bacterial lipopolysaccharide induces actin reorganisation, intercellular gap formation, and endothelial barrier dysfunction in pulmonary vascular endothelial cells: concurrent F-actin depolymerization and new actin synthesis, J. Cell. Physiol., 157 (1993) 13-23.
14. Goldblum, S.E., Ding, X. and Campbell-Washington, J., TNF-α induces endothelial cell F-actin depolymerization, new actin synthesis, and barrier dysfunction, Am. J. Physiol., 264 (1993) C894-C905.
15. Goto, I., Nagara, H., Tateishi, J. and Kuroiwa, Y., Thiamine-deficient encephalopathy in rats: effects of deficiencies of thiamine and magnesium, Brain Res., 372 (1986) 31-36.
16. Greenwood, J. and Pratt, O.E., Inhibition of thiamine transport across the blood-brain barrier in the rat by a chemical analogue of the vitamin, J. Physiol., 336 (1983) 479-486.
17. Greenwood, J. and Pratt, O.E., Comparison of the effects of some thiamine analogues upon thiamine transport across the blood-brain barrier of the rat, J. Physiol., 369 (1985) 79-91.
18. Hakim, A.M., The induction and reversibility of cerebral acidosis in thiamine deficiency, Ann. Neurol., 16 (1984) 673-679.
19. Harata, N. and Iwasaki, Y., Evidence for early blood-brain barrier breakdown in experimental thiamine deficiency in the mouse, Metab. Brain Dis., 10 (1995) 159-174.
20. Henry, R.J., Clinical Chemistry - Principles and Techniques, Harper and Row, New York (1968) pp. 664-666.
21. Kuhne, W., Besselmann, M., Noll, T., Muhs, A., Watanabe, H. and Piper, H.M., Disintegration of cytoskeletal structure of actin filaments in energy-depleted endothelial cells, Am J. Physiol., 264 (1993) H1599-H1608.
22. Madl, C., Kranz, A., Liebisch, B., Traindl, O., Lenz, K. and Druml, W., Lactic acidosis in thiamine deficiency, Clin. Nutr., 12 (1993) 108-111.
23. Majumdar, S.K., Shaw, G.K. and Thompson, A.D., Vitaminutilization status in chronic alcoholics, Int. J. Vitamin Nutr. Res., 51 (1981) 54-58.
24. Molina, P.E., Myers, N., Smith, R.M., Lang, C.H., Yousef, K.A., Tepper, P.G. and Abumrad, N.N., Nutritional and metabolic characterization of a thiamine-deficient rat model, J. Parent. Ent. Nutr., 18 (1994) 104-111.
25. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods, 65 (1983) 55-63.
26. Nag, S., Role of the endothelial cytoskeleton in blood-brain barrier permeability to protein, Acta Neuropathol., 90 (1995) 454-460.
27. Nobles, M., Revest, P.A., Couraud, P.-O. and Abbott, N.J., Characteristics of nucleotide receptors that cause elevation of cytoplasmic calcium in immortalized rat brain endothelial cells (RBE4) and in primary cultures, Br. J. Pharmacol., 115 (1995) 1245-1252.
28. Ray, D.E., Brown, A.W., Cavanagh, J.B., Nolan, C.C., Richards, H.K. and Wylie, S.P., Functional/metabolic modulation of the brain stem lesions caused by 1,3-dinitrobenzene in the rat, NeuroToxicology, 13 (1992) 379-388.
29. Rindi, G., Comincioli, V., Reggiani, C. and Patrini, C., Nervous tissue thiamine metabolism in vivo. III. Influence of ethanol intake on the dynamics of thiamine and its phosphoesters in different brain regions and sciatic nerve of the rat, Brain Res., 413 (1987) 23-25.
30. Rist, R.J., Romero, I.A. and Abbott, N.J., The effects of a cAMP agonist and astrocyte-conditioned medium on the F-actin cytoskeleton in cultured primary and immortalized rat brain capillary endothelial cells, J. Physiol., 480 (1994) 8-9P.
31. Rist, R.J., Romero, I.A., Chan, M.W.K., Couraud, P.-O., Roux, F. and Abbott, N.J., F-actin cytoskeleton and sucrose permeability of immortalized rat brain microvascular endothelial cell monolayers: effects of cAMP and astrocytic factors, Submitted (1996).
32. Rist, R.J., Romero, I.A., Chan, M.W.K. and Abbott, N.J., Effects of energy deprivation induced by fluorocitrate in immortalised rat brain microvessel endothelial cells, Brain Res., 730 (1996) 87-94.
33. Robertson, D.M. and Manz, H.J., Effect of thiamine deficiency on the competence of the blood-brain barrier to albumin labeled with fluorescent dyes, Am. J. Pathol., 63 (1971) 393-399.
34. 14C]-sucrose through an in vitro blood-brain barrier model, the RBE4 cell line, J. Physiol., (1994) 480, 8P.
35. Romero, I.A., Ray, D.E., Chan, M.W.K. and Abbott, N.J., An in vitro study of m-dinitrobenzene toxicity on the cellular components of the blood-brain barrier, astrocytes and endothelial cells, Toxicol. Appl. Pharm., 139 (1996) 94-101.
36. Romero, I.A., Rist, R.J., Chan, M.W.K. and Abbott, N.J., Acute energy deprivation syndromes: investigation of m-dinitrobenzene and α-chlorohydrin toxicity on immortalized rat brain microvessel endothelial cells, Neurotoxicology, in press.
37. Roux, F., Durieu-Trautmann, O., Chaverot, N., Claire, M., Mailly, P., Bourre, J.-M., Strosberg, A.D. and Couraud, P.-O., Regulation of gamma-glutamyl transpeptidase and alkaline phosphatase activities in immortalized rat brain microvessel endothelial cells, J. Cell. Physiol., 159 (1994) 101-113.
38. Rubin, L.L., Hall, D.E., Porter, S., Barbu, K., Cannon, C., Horner, H.C., Janatpour, C., Liaw, C.W., Manning, K., Morales, J., Tanner, L.I., Tomaselli, K.J. and Bard, F., A cell culture model of the blood-brain barrier, J. Cell Biol., 115 (1991) 1725-1735.
39. Shasby, D.M., Shasby, S.S., Sullivan, J.M. and Peach, M.J., Role of endothelial cell cytoskeleton in control of endothelial permeability, Circ. Res., 51 (1982) 657-661.
40. Takahashi, H., Nakazawa, S., Yoshino, Y. and Shimura, T., Metabolic studies of the edematous cerebral cortex of the pyrithiamine-treated thiamine-deficient rat, Brain Res., 441 (1988) 202-208.
41. Thomson, A.D., Ryle, P.R. and Shaw, G.K., Ethanol, thiamine and brain damage, Alcohol Alcoholism, 18 (1983) 27-43.
42. Thurston, G. and Turner, D., Thrombin-induced increase of F-actin in human umbilical vein endothelial cells, Microvasc. Res., 47 (1994) 1-20.
43. Watanabe, I., Tomita, T., Hung, K.-S. and Iwasaki, Y., Edematous necrosis in thiamine-deficient encephalopathy of the mouse, J. Neuropathol. Exp. Neurol., 40 (1981) 454-471.
44. Watanabe, I., Iwasaki, Y., Aikawa, H., Satoyoshi, E. and Davis, J.W., Hemorrhage of thiamine-deficient encephalopathy, J. Neuropathol. Exp. Neurol., 40 (1981) 566-580.