Adaptation of an automated selective dissociation procedure to two novel spheroid types [1]

In Vitro Cellular and Developmental Biology - Animal

Volumn 33, Issue 2, 1997, Pages 73-76

  Kunz Schughart, L A ^a   Freyer, J P ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CELL ISOLATION; CELL KINETICS; CELL PROLIFERATION; LETTER; SPHEROID CELL; TECHNIQUE; XENOGRAFT;

EID: 0030939878     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-997-0024-3     Document Type: Letter

References (17)

1. 1. M. J. Allalunis-Turner D. W. Siemann 1986 Recovery of cell subpopulations from human tumour xenografts following dissociation with different enzymes Br. J. Cancer 54 615 622 3535863 1:CAS:528:DyaL2sXivFChtQ%3D%3D Allalunis-Turner, M. J.; Siemann, D. W. Recovery of cell subpopulations from human tumour xenografts following dissociation with different enzymes. Br. J. Cancer 54:615–622; 1986.
2. 2. K. D. Bauer P. C. Keng R. M. Sutherland 1982 Isolation of quiescent cells from multicellular spheroids using centrifugal elutriation Cancer Res. 42 72 76 7032695 1:STN:280:DyaL38%2FptlWqtQ%3D%3D Bauer, K. D.; Keng, P. C.; Sutherland, R. M. Isolation of quiescent cells from multicellular spheroids using centrifugal elutriation. Cancer Res. 42:72–76; 1982.
3. 3. R. E. Durand 1975 Isolation of cell subpopulations from in vitro tumor models according to sedimentation velocity Cancer Res. 35 1295 1300 1168095 1:STN:280:DyaE2M7ivFWrtA%3D%3D Durand, R. E. Isolation of cell subpopulations from in vitro tumor models according to sedimentation velocity. Cancer Res. 35:1295–1300; 1975.
4. 4. R. E. Durand 1982 Use of Hoechst 33342 for cell selection from multicell systems J. Histochem. Cytochem. 30 111 116 7061816 1:CAS:528:DyaL38XktFyrs7c%3D Durand, R. E. Use of Hoechst 33342 for cell selection from multicell systems. J. Histochem. Cytochem. 30:111–116; 1982.
5. 5. J. P. Freyer R. M. Sutherland 1980 Selective dissociation and characterization of cells from different regions of multicell tumor spheroids Cancer Res. 40 3956 3965 7471046 1:STN:280:DyaL3M7jsFCqsA%3D%3D Freyer, J. P.; Sutherland, R. M. Selective dissociation and characterization of cells from different regions of multicell tumor spheroids. Cancer Res. 40:3956–3965; 1980.
6. 6. J. P. Freyer R. M. Sutherland 1986 Regulation of growth saturation and development of necrosis in EMT6/Ro multicell spheroids by the glucose and oxygen supply Cancer Res. 46 3504 3512 3708582 1:CAS:528:DyaL28XksFykurY%3D Freyer, J. P.; Sutherland, R. M. Regulation of growth saturation and development of necrosis in EMT6/Ro multicell spheroids by the glucose and oxygen supply. Cancer Res. 46:3504–3512; 1986.
7. 7. J. P. Freyer P. L. Schor 1989 Automated selective dissociation of cells from different regions of multicellular spheroids In Vitro Cell. Dev. Biol. 25 9 19 2914820 10.1007/BF02624405 1:STN:280:DyaL1M7it12msQ%3D%3D Freyer, J. P.; Schor, P. L. Automated selective dissociation of cells from different regions of multicellular spheroids. In Vitro Cell. Dev. Biol. 25:9–19; 1989.
8. 8. L. Goya C. P. Edwards K. A. Glennemeier 1991 In vivo effects of dexamethasone on the tumor growth of glucocorticoid-sensitive Fu5-derived rat hepatoma cells Cancer Lett. 58 211 219 1649693 10.1016/0304-3835(91)90103-O 1:CAS:528:DyaK3MXkvVGksrg%3D Goya, L.; Edwards, C. P.; Glennemeier, K. A., et al. In vivo effects of dexamethasone on the tumor growth of glucocorticoid-sensitive Fu5-derived rat hepatoma cells. Cancer Lett. 58:211–219; 1991.
9. 9. P. W. Hewett J. C. Murray E. A. Price 1993 Isolation and characterization of microvessel endothelial cells from human mammary adipose tissue In Vitro Cell. Dev. Biol. 29 325 331 10.1007/BF02633961 Hewett, P. W.; Murray, J. C.; Price, E. A., et al. Isolation and characterization of microvessel endothelial cells from human mammary adipose tissue. In Vitro Cell. Dev. Biol. 29:325–331; 1993.
10. 10. M. Kawata S. Sekiya K. Kera 1991 Neural rosette formation within in vitro spheroids of a clonal human teratocarcinoma cell line, PA-1/NM: role of extracellular matrix components in the morphogenesis Cancer Res. 51 2655 2669 2021944 1:CAS:528:DyaK3MXktVGks7s%3D Kawata, M.; Sekiya, S.; Kera, K., et al. Neural rosette formation within in vitro spheroids of a clonal human teratocarcinoma cell line, PA-1/NM: role of extracellular matrix components in the morphogenesis. Cancer Res. 51:2655–2669; 1991.
11. 11. P. C. Keng J. Allalalunis-Turner D. W. Siemann 1990 Evaluation of cell subpopulations isolated from human tumor xenografts by centrifugal elutriation Int. J. Radiot. Oncol. Biol. Phys. 18 1061 1067 1:STN:280:DyaK3c3ntVSiug%3D%3D 10.1016/0360-3016(90)90441-L Keng, P. C.; Allalalunis-Turner, J.; Siemann, D. W. Evaluation of cell subpopulations isolated from human tumor xenografts by centrifugal elutriation. Int. J. Radiot. Oncol. Biol. Phys. 18:1061–1067; 1990.
12. 12. L. A. Kunz-Schughart A. Simm W. Mueller-Klieser 1995 Oncogene-associated transformation of rodent fibroblasts is accompanied by large morphologic and metabolic alterations Oncology Reports 2 651 661 1:CAS:528:DyaK2MXmslymu7s%3D Kunz-Schughart, L. A.; Simm, A.; Mueller-Klieser, W. Oncogene-associated transformation of rodent fibroblasts is accompanied by large morphologic and metabolic alterations. Oncology Reports 2:651–661; 1995.
13. 13. D. A. Loeffler P. C. Keng R. B. Baggs 1990 Lymphocytic infiltration and cytotoxicity under hypoxic conditions in the EMT6 mouse mammary tumor Int. J. Cancer 45 462 467 2307536 10.1002/ijc.2910450315 1:STN:280:DyaK3c7ntVWqtg%3D%3D Loeffler, D. A.; Keng, P. C.; Baggs, R. B., et al. Lymphocytic infiltration and cytotoxicity under hypoxic conditions in the EMT6 mouse mammary tumor. Int. J. Cancer 45:462–467; 1990.
14. 14. W. Mueller-Klieser 1987 Multicellular spheroids: a review on cellular aggregates in cancer research J. Cancer Res. Clin. Oncol. 113 101 122 3549738 10.1007/BF00391431 1:STN:280:DyaL2s7ms1WhsQ%3D%3D Mueller-Klieser, W. Multicellular spheroids: a review on cellular aggregates in cancer research. J. Cancer Res. Clin. Oncol. 113:101–122; 1987.
15. 15. Paulus, W.; Huettner, C.; Tonn, J. C. Collagens, integrins, and the mesenchymal drift in glioblastomas: a comparison of biopsy specimens, spheroid, and early monolayer cultures. Int. J. Cancer 841–846; 1994.
16. 16. R. M. Sutherland 1988 Cell and environment interactions in tumor microregions: the multicell spheroid model Science 240 177 184 2451290 10.1126/science.2451290 1:STN:280:DyaL1c7nvVSrtw%3D%3D Sutherland, R. M. Cell and environment interactions in tumor microregions: the multicell spheroid model. Science 240:177–184; 1988.
17. 17. Y. Wang D. A. Mathers 1993 Ca 2+-dependent K +-channels of high conductance in smooth muscle cells isolated from rat cerebral arteries J. Physiol. 462 529 545 8331591 1:STN:280:DyaK3szitlShug%3D%3D Wang, Y.; Mathers, D. A. Ca 2+-dependent K +-channels of high conductance in smooth muscle cells isolated from rat cerebral arteries. J. Physiol. 462:529–545; 1993.