Staining technique to differentiate mineralized and demineralized bone in ground sections

Journal of Histotechnology

Volumn 20, Issue 2, 1997, Pages 119-122

  Sanderson, Cathy ^a   Bachus, Kent N ^b  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

Bone; Demineralized; Ground histology; Methyl methacrylate; Mineralized; Rapid bone stain

Indexed keywords

METHACRYLIC ACID METHYL ESTER; PARAFFIN;

ARTICLE; BONE MINERALIZATION; CLINICAL ARTICLE; CONNECTIVE TISSUE; DEMINERALIZATION; DONOR; GRAFT REJECTION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; PREOPERATIVE EVALUATION; STAINING;

EID: 0030939356     PISSN: 01478885     EISSN: None     Source Type: Journal    
DOI: 10.1179/his.1997.20.2.119     Document Type: Article

References (29)

1. Shen W-J, Chung K-C, Wang G-J et al: Demineralized bone matrix in the stabilization of porous-coated implants in bone defects in rabbits. Clin Orthop 293:346-352, 1993
2. Urist MR: Bone: Formation by autoinduction. Science 150: 893-899, 1965
3. Pajamäki KJJ, Andersson ÖH, Lindholm TS et al: Effect of glass bioactivity on new bone development induced by demineralized bone matrix in a rat extraskeletal site. Arch Orthop Trauma Surg 113:210-214, 1994
4. Pajamàki KJJ, Andersson OH, Lindholm TS et al: Effect of bovine bone morphogenetic protein and bioactive glass on demmeralized bone matrix grafts in the rat muscular pouch. Ann Chir Gynecol Suppl 207:155-161, 1993
5. Wolfe MW, Cook SD: Use of osteoinductive implants in the treatment of bone defects. Med Prog Technol 20:155-168, 1994
6. Reddi AH, Cunningham NS: Bone induction by osteogenin and bone morphogenetic proteins. Biomaterials 11:33-34, 1990
7. Reddi AH, Cunningham NS: Recent progress in bone induction by osteogenin and bone morphogenetic proteins: challenges for biomechanical and tissue engineering. J Biomech Eng 1 13:189-190, 1991
8. Reddi AH: Regulation of cartilage and bone differentiation by bone morphogenetic proteins. Curr Opin Cell Biol 4:850-855, 1992
9. Reddi AH, Cunningham NS: Initiation and promotion of bone differentiation by bone morphogenetic proteins. J Bone Miner Res 8(Suppl 2):S499-502, 1993
10. Sailer HF, Kolob E: Application of purified bone morphogenetic protein (BMP) in craniomaxillo-facial surgery. J Craniomaxillofac Surg 22:2-11, 1994
11. Schwarz N, Schlag G, Thurnher M et al: Decalcified and undecalcified cancellous bone block implants do not heal diaphyseal defects in dogs. Arch Orthop Trauma Surg 111:47-50, 1991
12. Nishibori M, Betts NJ, Salama H et al: Short-term healing of autogenous and allogeneic bone grafts after sinus augmentation: A report of 2 cases. J Periodontal 65:958-966, 1994
13. Recker RR: Bone Histomorphometry: Techniques and Interpretation. CRC Press, Boca Raton, 1983, pp 306
14. Dickson GR: Methods of calcified tissue preparation. In Methods of Calcified Tissue Preparation. Elsevier, Amsterdam, 1984, pp 123-127
15. Schenk RK, Olah AJ, Herrmann W: Preparation of calcified tissues for light microscopy. In Methods of Calcified Tissue Preparation, GR Dickson (ed). Elsevier, New York, 1984, pp 1-56
16. Villanueva AR, Lundin KD: A versatile new mineralized bone stain for simultaneous assessment of tetracycline and osteoid seams. Stain Technol 64:129-138, 1989
17. Eurell JAC, Sterchi DL: Microwaveable toluidine blue stain for surface staining of undecalcified bone sections. J Histotechnol 17:357-359, 1994
18. Sanderson C, Bloebaum RD: Advances in the staining of ground section histology. Histo-Logic XXIII:1-3, 1993
19. Hofmann AA, Bloebaum RD, Rubman MH et al: Microscopic analysis of autograft bone applied at the interface of porous-coated devices in human cancellous bone. Int Orthop (SIROT Prize) 16:349-358, 1992
20. Sanderson C, Kitabayashi LR: Parallel experience of two different laboratories with the initiator perkadox 16 for polymerization of methylmethacrylates. J Histotechnol 17:343-348, 1994
21. Sanderson C, McGee M, Bloebaum RD: Polypropylene containers for safe and predictable embedding of specimens in PMMA. J Histotechnol 13:131-133, 1990
22. Bachus KN, Bloebaum RD: Projection effect errors in biomaterials and bone research. Cells Mater 2:347-355, 1992
23. Hofmann AA, Bachus KN, Bloebaum RD: Comparative study of human cancellous bone remodeling to titanium and hydroxyapatite coated implants. J Arthroplasty 8:157-166, 1993
24. Bloebaum RD, Sanderson C, McCarvill S et al: Plastic slides in the preparation of implant and tissue for interface analysis. J Histotechnol 12:307-310, 1989
25. Luna LG (ed): Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3rd ed. McGraw Hill, New York, 1968, p 81
26. Robinson VNE: Imaging with backscattered electrons in a scanning electron microscope. Scanning 3:15-26, 1980
27. Bloebaum RD, Bachus KN, Boyce TM: Backscattered electron imaging: The role in calcified tissue and implant analysis. J Biomater Appl 5:56-85, 1990
28. Skedros JG, Bloebaum RD, Bachus KN et al: The meaning of graylevels in backscattered electron images of bone. J Biomed Mater Res 27:47-56, 1993
29. Skedros JG, Bloebaum RD, Bachus KN et al: Influence of mineral content and composition on graylevels in backscattered electron images of bone. J Biomed Mater Res 27:57-64, 1993