In vitro confirmation of newly established lung cancer cell lines using flow cytometry and multicellular tumor spheroids

Lung Cancer

Volumn 17, Issue 1, 1997, Pages 85-101

  Inoue, Shoichi ^a   Takaoka, Kazuo ^b   Endo, Takashi ^c   Mizuno, Shigetaka ^a   Ogawa, Yoshihiko ^d   Yoshida, Michihiro ^a   Ohnuma, Takao ^e  

^a HOKKAIDO UNIVERSITY   (Japan)

^b Nikko Memorial Hospital   (Japan)

^c HOKKAIDO UNIVERSITY HOSPITAL   (Japan)

^d HOKKAIDO UNIVERSITY   (Japan)

^e MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

cytopathological type; DNA; electronmicrograph; flow cytometry; karyotype; multicellular tumor spheroids; tumor associated antigen

Indexed keywords

DNA; FLUORESCEIN ISOTHIOCYANATE; OSMIUM TETRAOXIDE; PARAFORMALDEHYDE; PROPIDIUM IODIDE; TUMOR ANTIGEN;

ANCHORAGE INDEPENDENT GROWTH; ARTICLE; CANCER CELL CULTURE; CELL STRUCTURE; CLINICAL ARTICLE; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE; FROZEN SECTION; HUMAN; HUMAN CELL; HUMAN TISSUE; LUNG CANCER; MULTICELLULAR SPHEROID; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; TISSUE FIXATION; TRANSMISSION ELECTRON MICROSCOPY;

ANTIGENS, NEOPLASM; CARCINOEMBRYONIC ANTIGEN; CARCINOMA, SQUAMOUS CELL; FLOW CYTOMETRY; HISTOCYTOCHEMISTRY; HUMANS; IMMUNOHISTOCHEMISTRY; KARYOTYPING; LUNG; LUNG NEOPLASMS; MICROSCOPY, ELECTRON; PHOSPHOPYRUVATE HYDRATASE; PHOTOGRAPHY; SPHEROIDS, CELLULAR; TUMOR CELLS, CULTURED;

EID: 0030938618     PISSN: 01695002     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-5002(97)00652-1     Document Type: Article

References (36)

1. Bergh J, Esscher T, Steinholtz L, Nilson K, Pahlman S. Immunocytochemical demonstration of neuron-specific enolase (NSE) in human lung cancers. Am J Clin Pathol 1985; 84: 1-7.
2. Berner A, Jacobsen AB, Fossa SD, Nesland JM. Expression of c-erb B-2 protein, neuron-specific enolase and DNA flow cytometry in locally advanced transitional cell carcinoma of the urinary bladder. Histopathology 1993; 22: 327-333.
3. Carney DN, Mitchell JB, Kinsella TJ. In vitro radiation and chemotherapy sensitivity of established cell lines of human small cell lung cancer and its large cell morphological variants. Cancer Res 1983; 43: 2806-2811.
4. Carney DN, Zweig MH, Ihde DC, Cohen MH, Makuch RW, Gazdar AF. Elevated serum creatinine kinase-BB levels in patients with small cell lung cancer. Cancer Res 1984; 44: 5399-5403.
5. Durand RE. Cell cycle kinetics in an in vitro tumor model. Cell Tissue Kinet 1976; 9: 403-412.
6. Durand RE. Variable radiobiological responses of spheroids. Radiat Res 1980; 81: 85-99.
7. Franko AJ, Sutherland RM. Radiation survival of cells from spheroids grown in different oxygen concentrations. Radiat Res 1979; 79: 454-467.
8. Freshney RI. Characterization. In: Freshney RI, editor. Culture of Animal Cells. A Manual of Basic Technique (2nd Edition). New York: Wiley-Liss, 1987; 169-185.
9. Giovanella BC, Vardeman DM, Williams LJ, Taylor DJ, de Ipolyi PD, Greeff PJ, Stehlin, JS, Ullrich A, Cailleau R, Slamon DJ, Gary Jr. HE. Heterotransplantation of human breast carcinomas in nude mice. Correlation between successful heterotransplants, poor prognosis and amplification of the HER-2/neu oncogene. Int J Cancer 1991; 47: 66-71.
10. ICSN. An international system for human cytogenetic nomenclature. In: Harnden DG, Klinger HP, editors, published in collaboration with Cytogent. Cell Genet. Karger: Basel, 1985.
11. Inoue S, Ito M, Mizuno S, Isobe H, Miyamoto H, Kawakami Y Ohnuma T. Simultaneous flow cytometric detection of nuclear DNA and tumor-associated antigens in lung cancer. Anal Quant Cytol Histol 1988; 10: 243-250.
12. Inoue S, Ohnuma T. Effects of physiological oxygen environment on drug-induced cell lethality of multicellular tumor spheroids from human lung cancer. Sel Cancer Ther 1989; 5: 13-22.
13. Inoue S, Ohnuma T, Takaoka K, Suzuki Y Kaneko M, Safirstein R, Holland JF. Effects of doxorubicin and cisplatin on multicellular tumor spheroids from human lung cancer. Cancer Drug Deliv 1987; 4: 213-224.
14. Johnson DH, Marangos PJ, Forbes JT, Hainsworth JD, Van Welch R, Hande KR, Greco FA. Potential utility of serum neuron-specific enolase in small cell carcinoma of the lung. Cancer Res 1984; 44: 5409-5414.
15. Kaufmann SH, Mabry M, Jasti R, Shaper JH. Differential expression of nuclear envelope lamins A and C in human lung cancer cell line. Cancer Res 1991; 51: 581-586.
16. Kodama Y Yoshida MC, Sasaki M. An improved silver staining technique for nucleolus organizer regions by using nylon cloths. Jpn J Hum Gent 1980; 25: 229-233.
17. Ludwig CU, Raefle G, Dalquen P Stulz P Stahel R, Obrecht JP. Allelic loss on the short arm of chromosome 11 in non-small-cell lung cancer. Int J Cancer 1991; 49: 661-665.
18. Maehara Y, Sugimachi K, Akagi M, Kakegawa T, Shimazu H, Tomita M. Serum carcinoembry-onic antigen level increases correlation with tumor progression in patients with differentiated gastric carcinoma following noncurative resection. Cancer Res 1990; 50: 3952-3955.
19. Mendelsohn G, Baylin SB. Time-dependent changes in human tumors: implications for diagnosis and clinical behavior. In: Fer MF, Greco FA, Oldham RK, editors. Poorly Differentiated Neoplasms and Tumors of Unknown Origin. Orlando; Grune and Stratton, 1986; 379-397.
20. Mori N, Yokota J, Oshimura M, Cavenee WK, Mizoguchi H, Noguchi M, Shimosato Y, Sugimura T, Terada M. Concordant deletion of chromosome 3p and loss of heterozygosity for chromosomes 13 and 17 in small cell lung carcinoma. Cancer Res 1989; 49: 5130-5135.
21. Niklinski J, Furman M, Laudanski J, Kozlowski M. Evaluation of squamous cell carcinoma antigen (SCC-Ag) in the diagnosis and follow-up of patients with non-small cell lung carcinoma. Neoplasma 1992; 39: 279-282.
22. Putman DL, Park DK, Rhim JS, Ting RC. Correlation of cellular aggregation of transformed cells with their growth in soft agar and tumorigenic potential. Proc Soc Expl Biol Med 1977; 155: 487-494.
23. Said JW, Vimadalal S, Nash G, Shintaku IP, Heusser PC, Sassoon AF, Lloyd, RV. Immunoreactive neuron-specific enolase, bombesin, and chromogranin as markers for neurnendocrine lung tumors. Hum Pathol 1985; 16: 236-240.
24. Sasaki T, Yamamoto M, Yamaguchi T, Sugiyama S. Development of multicellular spheroids of Hela cells cocultured with fibroblasts and their response to X-irradiation. Cancer Res 1984 44: 345-351.
25. Sato S, Nakamura Y Tsuchiya E. Difference of allelotype between squamous cell carcinoma and adenocarcinoma of the lung. Cancer Res 1994; 54: 5652-5655.
26. Steuer AF, Rhim JS, Hentosk PM, Ting RC. Survival of human cells in the aggregate form: potential index of in vitro cell transformation. J Natl Cancer Inst 1977; 58: 917-921.
27. Sundaresan V, Reeve JG, Stenning S, Stewart S, Bleehen NM. Neuroendocrine differentiation and clinical behaviour in non-small cell lung tumors. Br J Cancer 1991; 64: 333-338.
28. Sutherland RM, Durand RE. Radiation response of multicell spheroids. An in vitro tumor model. Curr Top Radiat Res 1976; 11: 87-139.
29. Tamai M, Kanimura H, Yamaue H, Iwahashi M, Tsunoda T, Tani M, Noguchi K, Hotta T, Arii K. Expression of carcinoembryonic antigen in fresh human gastric cancer cells assessed by flow cytometry. J Surg Oncol 1993; 52: 176-180.
30. Tome Y, Hirohashi S, Noguchi M, Matsuno Y, Kishi K, Uei Y, Shimosato Y Immunocytologic diagnosis of small-cell lung cancer in imprint smears. Acta Cytol 1991; 35: 485-490.
31. Umezawa Y, Kuge S, Kikyo N, Shirai T, Watanabe J, Fujiwara M, Okabe T. Identity of brain-associated small cell lung cancer antigen and the CD56 (NKH-1/Leu19) leukocyte differentiation antigen and the neural cell adhesion molecule. Jpn J Clin Oncol 1991; 21: 251-255.
32. Von Hoff DD, Clark, GM, Stogdill BJ, Sarosdy MF, O'Brien MT, Casper JT, Mattox DE, Page CP, Cruz AB, Sandbach JF. Prospective clinical trial of a human tumor cloning system. Cancer Res 1983; 43: 1926-1931.
33. Whang Peng J, Knutsen T, Gazda A, Steinberg SM, Oie H, Linnoila 1, Mulshine J, Nau M, Minna JD. Nonrandom structural and numerical chromosome changes in non-small-cell lung cancer. Genes Chromosom Cancer 1991; 3: 168-188.
34. Wong YC, Bernai SD. Expression of enhancer binding factors associated with various cell types of lung cancer. Biochem Biophys Res Commun 1991; 180: 1064-1069.
35. Yuhas JM, Li AP. Growth fraction as the major determination of multicellular tumor spheroid growth rate. Cancer Res 1978; 38: 1528-1532.
36. Yuhas JM, Tarleton AE, Molzen KB. Multicellular tumor spheroid formation by breast cancer cells isolated from different sites. Cancer Res 1978; 38: 2486-2491.