Direct PCR from paraffin-embedded tissue

BioTechniques

Volumn 22, Issue 4, 1997, Pages 638-640

  Burns, W C ^a   Liu, Y S ^a   Dow, C ^a   Thomas, R J S ^a   Phillips, W A ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; COLON; DNA PURIFICATION; GENE AMPLIFICATION; GOOD LABORATORY PRACTICE; HUMAN; HUMAN TISSUE; ONCOGENE K RAS; POLYMERASE CHAIN REACTION; TISSUE SECTION;

EID: 0030938498     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97224bm13     Document Type: Article

References (3)

1. Kallio, P., S. Syrjanen, A. Tervahauta and K. Syrjanen. 1991. A simple method for isolation of DNA from formalin-fixed paraffin-embedded samples for PCR. J. Virol. Method 35:39-47.
2. Liu, Y.S., R.J.S. Thomas and W.A. Phillips. 1995. Single-step direct PCR amplification from solid tissues. Nucleic Acids Res. 23:1640.
3. Singer-Sam, J., R.L. Tanguary and A. Rigg. 1990. Use of chelex to improve the polymerase chain reaction signal from a small number of cells. Amplification 3:11.