Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum

European Journal of Biochemistry

Volumn 244, Issue 3, 1997, Pages 862-868

  Tersteegen, Adrian ^a   Linder, Dietmar ^b   Thauer, Rudolf K ^a   Hedderich, Reiner ^a,c  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b JUSTUS LIEBIG UNIVERSITY   (Germany)

^c MAX PLANCK INSTITUTE FOR TERRESTRIAL MICROBIOLOGY   (Germany)

Author keywords

2 Oxoacid oxidoreductase; Methanobacterium thermoautotrophicum; Methanogenic Archaea; Pyrococcus furiosus; Pyruvate oxidoreductase

Indexed keywords

2 OXOACID; OXIDOREDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STRUCTURE; METHANOBACTERIUM THERMOAUTOTROPHICUM; NONHUMAN; PRIORITY JOURNAL;

ARCHAEA; METHANOBACTERIUM; METHANOTHERMOBACTER THERMAUTOTROPHICUS; PYROCOCCUS; PYROCOCCUS FURIOSUS; THERMOCOCCUS SP.;

EID: 0030937675     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00862.x     Document Type: Article

References (37)

1. Betner, I. & Foldi, P. (1988) The FMOC-ADAM approach to amino acid analysis, LC-GC Int. 2, 44-53.
2. Blamey, J. M. & Adams, M. W. W. (1993) Purification and characterization of pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus, Biochim. Biophys. Acta 1161, 19-27.
3. Bock, A.-K., Kunow, J., Glasemacher, J. & Schönheit, P. (1996) Catalytic properties, molecular composition and sequence alignments of pyruvate:ferredoxin oxidoreductase from the methanogenic archaeon Methanosarcina barkeri (strain Fusaro), Eur. J. Biochem. 237, 35-44.
4. Bokranz, M., Bäumner, G., Allmansberger, R., Ankel-Fuchs, D. & Klein, A. (1988) Cloning and characterization of the methyl coenzyme M reductase genes from Methanobacterium thermoautotrophicum, J. Bacteriol. 170, 568-577.
5. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 248-254.
6. Brandis, A., Thauer, R. K. & Stetter, K. O. (1981) Relatedness of strains ΔH and Marburg of Methanobacterium thermoautotrophicum, Zentralbl. Bakteriol. Mikrobiol. Hyg. I Abt. Orig. C 2, 311-317.
7. Brostedt, E. & Nordlund, S. (1991) Purification and partial characterization of a pyruvate oxidoreductase from the photosynthetic bacterium Rhodospirillum rubrum grown under nitrogen-fixing conditions, Biochem. J. 279, 155-158.
8. Bult, C. J., White, O., Olsen, G. J., Zhou, L., Fleischmann, R. D., Sutton, G. G., Blake, J. A., FitzGerald, L. M., Clayton, R. A., Gocayne, J. D., Kerlavage, A. R., Dougherty, B. A., Tomb, J.-F., Adams, M. D., Reich, C. I., Overbeek, R., Kirkness, E. F., Weinstock, K. G., Merrik, J. M., Glodek, A., Scott, J. L., Geoghagen, N. S. M., Weidman, J. F., Fuhrmann, J. L., Nguyen, D., Utterback, T. R., Kelley, J. M. Peterson, J. D., Sadow, P. W., Hanna, M. C., Cotton, M. D., Roberts, K. M., Hurst, M. A., Kaine, B. P., Borodovsky, M., Klenk, H.-P., Fraser, C. M., Smith, H. O., Woese, C. R. & Venter, J. C. (1996) Complete genome sequence of the methanogenic Archaeon, Methanococcus jannaschii, Science 273, 1058-1073.
9. Eikmanns, B., Jaenchen, R. & Thauer, R. K. (1983) Propionate assimilation by methanogenic bacteria, Arch. Microbiol. 136, 106-110.
10. Fuchs, G., Stupperich, E. & Thauer, R. K. (1978) Acetate assimilation and synthesis of alanine, aspartate and glutamate in Methanobacterium thermoautotrophicum, Arch. Microbiol. 117, 61-66.
11. 2 fixation in Autotrophic bacteria (Schlegel, H. G. & Bowien, B., eds) pp. 365-382, Science Tech. Publishers, Madison, WI, and Springer-Verlag, Berlin.
12. Gehring, U. & Arnon, D. I. (1972) Purification and properties of α-ketoglutarate synthase from a photosynthetic bacterium, J. Biol. Chem. 247, 6963-6969.
13. Heider, J., Ma, K. & Adams, M. W. W. (1995) Purification, characterization, and metabolic function of tungsten-containing aldehyde ferredoxin oxidoreductase from the hyperthermophilic and proteolytic archaeon Thermococcus strain ES-1, J. Bacteriol. 177, 4757-4764.
14. Heider, J., Mai, X. & Adams, M. W. W. (1996) Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea, J. Bacteriol. 178, 780-787.
15. Kerscher, L. & Oesterhelt, D. (1981) Purification and properties of two 2-oxoacid:ferredoxin oxidoreductases from Halobacterium halobium, Eur. J. Biochem. 116, 587-594.
16. Kletzin, A. & Adams, M. W. W. (1996) Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima, J. Bacteriol. 178, 248-257.
17. Kunow, J., Linder, D. & Thauer, R. K. (1995) Pyruvate:ferredoxin oxidoreductase from the sulfate-reducing Archaeglobus fulgidus: molecular composition, catalytic properties, and sequence alignments, Arch. Microbiol. 163, 21-28.
18. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
19. 2 in Methanobacterium thermoautotrophicum. Synthesis from tetrahydomethanopterin-bound C1 units and carbon monoxide, Eur. J. Biochem. 163, 147-154.
20. Mai, X. & Adams, M. W. W. (1994) Indolepyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus, J. Biol. Chem. 269, 16726-16732.
21. 4MPT dehydrogenase gene from Methanobacterium thermoautotrophicum strain Marburg and functional expression in E. coli, J. Biol. Chem. 270, 2827-2832.
22. Nölling, J., Hahn, D., Ludwig, W. & de Vos, W. M. (1993) Phylogenetic analysis of thermophilic Methanobacterium sp.: evidence for a formate-utilizing ancestor, System. Appl. Microbiol. 16, 208-215.
23. Nölling, J., Pihl, T. D., Vriesema, A. & Reeve, J. N. (1995a) Organization and growth phase-dependent transcription of methane genes in two regions of the Methanobacterium thermoautotrophicum genome, J. Bacteriol. 177, 2460-2468.
24. 10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum ΔH and Methanopyrus kandleri, J. Bacteriol. 177, 7238-7244.
25. 2 in methanogenesis in Methanobacterium thermoautotrophicum, Eur. J. Biochem. 231, 628-638.
26. Oberlies, G., Fuchs, G. & Thauer, R. K. (1980) Acetate thiokinase and the assimilation of acetate in Methanobacterium thermoautotrophicum, Arch. Microbiol. 128, 248-252.
27. 5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum ΔH, J. Bacteriol. 176, 6384-6391.
28. Roberts, R. B., Abelson, P. H., Cowie, D. B., Bolton, E. T. & Britton, R. J. (1957) Studies of biosynthesis in Escherichia coli, Carnegie Institute, Washington.
29. Schäfer, T., Selig, M. & Schönheit, P. (1993) Acetyl-CoA synthetase (ADP forming) in archaea, a novel enzyme involved in acetate formation and ATP synthesis, Arch. Microbiol. 159, 72-83.
30. s) of Methanobacterium thermoautotrophicum, Arch. Microbiol. 127, 59-65.
31. Uyeda, K. & Rabinowitz, J. C. (1971) Pyruvate-ferredoxin oxidoreductase. III. Purification and properties of the enzyme, J. Biol. Chem. 246, 3111-3119.
32. 10-methylenetetrahydromethanopterin reductase (Mer) from Methanobacterium thermoautotrophicum strain Marburg. Cloning, sequencing, transcriptional analysis, and functional expression in Escherichia coli of the mer gene, Eur. J. Biochem. 231, 773-778.
33. 420-reducing methylene tetrahydromethanopterin dehydrogenase are genetically distinct enzymes in Methanobacterium thermoautotrophicum (Marburg), Eur. J. Biochem. 202, 1205-1208.
34. Wasserfallen, A., Huber, K. & Leisinger, T. (1995) Purification and structural characterization of a flavoprotein induced by iron limitation in Methanobacterium thermoautotrophicum Marburg, J. Bacteriol. 177, 2436-2441.
35. Williams, K., Lowe, P. N. & Leadlay, P. F. (1987) Purification and characterization of pyruvate:ferredoxin oxidoreductase from the anaerobic protozoon Trichomonas vaginalis, Biochem. J. 246, 529-536.
36. Zeikus, J. G., Fuchs, G., Kenealy, W. & Thauer, R. K. (1977) Oxidoreductases involved in cell carbon synthesis of Methanobacterium thermoautotrophicum, J. Bacteriol. 132, 604-613.
37. Zhang, Q., Iwasaki, T., Wakagi, T. & Oshima, T. (1996) 2-Oxoacid-ferredoxin oxidoreductases from the thermoacidophilic archaeon, Sulfolobus SP strain 7, J. Biochem. (Tokyo) 120, 587-599.