Alz-50 and MC-1, a new monoclonal antibody raised to paired helical filaments, recognize conformational epitopes on recombinant tau

Journal of Neuroscience Research

Volumn 48, Issue 2, 1997, Pages 128-132

  Jicha, Gregory A ^a   Bowser, Robert ^b   Kazam, Imrana G ^a   Davies, Peter ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

Alzheimer's disease; conformation; FAC1

Indexed keywords

MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY ALZ 50; MONOCLONAL ANTIBODY MC 1; MUTANT PROTEIN; RECOMBINANT PROTEIN; TAU PROTEIN; UNCLASSIFIED DRUG;

ALZHEIMER DISEASE; ANTIGEN DETECTION; ANTIGEN RECOGNITION; ARTICLE; BRAIN HOMOGENATE; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; PROTEIN CONFORMATION;

ANTIBODIES, MONOCLONAL; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; ANTIGENS, NUCLEAR; EPITOPE MAPPING; EPITOPES; HUMANS; MUTAGENESIS, SITE-DIRECTED; NERVE TISSUE PROTEINS; NEUROFIBRILLARY TANGLES; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; TAU PROTEINS; TRANSCRIPTION FACTORS;

EID: 0030936599     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4547(19970415)48:2<128::AID-JNR5>3.0.CO;2-E     Document Type: Article

References (12)

1. Bowser R, Giambrone A, Davies P (1995): FAC1, a novel gene identified with the monoclonal antibody Alz50, is developmentally regulated in human brain. Dev Neurosci 17:20-37.
2. Braak E, Braak H, Mandelkow EM (1994): A sequence of cytoskeleton changes related to the formation of neurofibrillary tangles and neuropil threads. Acta Neuropathol 87:554-567.
3. Goedert M, Spillantini MG, Jakes R, Rutherford D, Crowther RA (1989): Multiple isoforms of human microtubule-associated protein-tau: Sequences and localization in neurofibrillary tangles of Alzheimer's-disease. Neuron 3:519-526.
4. Goedert M, Spillantini MG, Jakes R (1991): Localization of the Alz-50 epitope in recombinant human microtubule-associated protein tau. Neurosci Lett 126:149-154.
5. Ksiezak-Reding H, Davies P, Yen SH (1988): Alz 50, a monoclonal antibody to Alzheimer's disease antigen, cross-reacts with tau protein from bovine and normal human brain. J Biol Chem 263:7943-7947.
6. Ksiezak-Reding H, Leibowitz RL, Bowser B, Davies P(1995): Binding of Alz-50 depends on Phe8 in tau synthetic peptides and varies between native and denatured tau proteins. Brain Res 697: 63-75.
7. Schweers O, Schonbrunn-Hanebeck E, Marx A, Mandelkow E (1994): Structural studies of tau protein and Alzheimer paired helical filaments show no evidence for B-structure. J Biol Chem 269:24290-24297.
8. Ueda K, Masliah E, Saitoh T, Bakalis SL, Scoble H, Kosik KS (1990): Alz-50 recognizes a phosphorylated epitope of tau protein. J Neurosci 10:3295-3304.
9. Wille H, Drewes G, Biernat J, Mandelkow EM, Mandelkow E (1992): Alzheimer-like paired helical filaments and antiparallel dimers formed from microtubule-associated protein tau in vitro. J Cell Biol 118:573-584.
10. Wolozin BL, Davies P (1987): Alzheimer-related neuronal protein A68: Specificity and distribution. Ann Neurol 22:521-526.
11. Wolozin BL, Pruchnicki A, Dickson DW, Davies P (1986): A neuronal antigen in the brains of Alzheimer patients. Science 232:648-650.
12. Wolozin BL, Scicutella A, Davies P (1988): Reexpression of a developmentally regulated antigen in Down syndrome and Alzheimer disease. Proc Natl Acad Sci USA 85:6202-6206.