Ability of Streptomyces spp. acyl carrier proteins and coenzyme A analogs to serve as substrates in vitro for E. coli holo-ACP synthase

Chemistry and Biology

Volumn 4, Issue 1, 1997, Pages 17-24

  Gehring, Amy M ^a   Lambalot, Ralph H ^a   Vogel, Kurt W ^b   Drueckhammer, Dale G ^b   Walsh, Christopher T ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

acyl carrier protein; holo ACP synthase; phosphopantetheine; phosphopantetheinyl transferase; polyketide synthase

Indexed keywords

ESCHERICHIA COLI; STREPTOMYCES;

EID: 0030936547     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(97)90233-7     Document Type: Article

References (19)

1. Hopwood, D.A. & Sherman, D.H. (1990). Molecular genetics of polyketides and its comparison to fatty acid biosynthesis. Annu. Rev. Genet. 24, 37-66.
2. Hutchinson, C.R. (1995). Polyketide synthase gene manipulation: a structure-function approach in engineering novel antibiotics. Annu. Rev. Microbiol. 49, 201-238.
3. Summers, R.G., Ali, A., Shen, B., Wessel, W.A. & Hutchinson, C.R. (1995). Malonyl-coenzyme A: acyl carrier protein acyltransferase of Streptomyces glaucescens: a possible link between fatty acid and polyketide biosynthesis. Biochemistry 34, 9389-9402.
4. Revill, W.P., Bibb, M.J., & Hopwood, D.A. (1996). Relationships between fatty acid and polyketide syntheses from Streptomyces coelicolor A3(2): characterization of the fatty acid synthase acyl carrier protein. J. Bacteriol. 178, 5660-5667.
5. Shen, B., Summers, R.G., Gramajo, H., Bibb, M.J. & Hutchinson, C.R. (1992). Purification and characterization of the acyl carrier protein of the Streptomyces glaucescens tetracenomycin C polyketide synthase. J. Bacteriol. 174, 3818-3821.
6. Crosby, J., Sherman, D.H., Bibb, M.J., Revill, W.P., Hopwood, D.A. & Simpson, T.J. (1995). Polyketide synthase acyl carrier proteins from Streptomyces: expression in Escherichia coli, purification and partial characterisation. Biochim. Biophys, Acta 1251, 35-42.
7. Lambalot, R.H. & Walsh, C.T. (1995). Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase. J. Biol. Chem. 270, 24658-24661.
8. Hill, R.B., MacKenzie, K.R., Flanagan, J.M., Cronan, J.E., Jr. & Prestegard, J.H. (1995). Overexpression, purification, and characterization of Escherichia coli acyl carrier protein and two mutant proteins. Protein Expr. Purif. 6, 394-400.
9. Lambalot, R.H. & Walsh, C.T. (1997). Holo-acyl carrier protein synthase of Escherichia coli. Methods Enzymol., in press.
10. Debabov, D.V., Heaton, M.P., Zhang, Q., Stewart, K.D., Lambalot, R.H. & Neuhaus, F.C. (1996). The D-alanyl carrier protein in Lactobacillus casei: cloning, sequencing, and expression of dltC. J. Bacteriol. 178, 3869-3876.
11. Lambalot, R.H., et al., & Walsh, C.T. (1996). A new enzyme superfamily - the phosphopantetheinyl transferases. Chemistry & Biology 3, 923-936.
12. Stachelhaus, T., Hüser, A. & Marahiel, M.A. (1996). Biochemical characterization of peptidyl carrier protein (PCP), the thiolation domain of multifunctional peptide synthetases. Chemistry & Biology 3, 913-921.
13. Revill, W,P,. Bibb, M.J. & Hopwood, D.A. (1995). Purification of a malonyltransferase from Streptomyces coelicolor A3(2) and analysis of its genetic determinant. J. Bacteriol. 177, 3946-3952.
14. Morris, S.A., Revill, W.P., Staunton, J. & Leadlay, P.F. (1993). Purification and separation of holo- and apo-forms of Saccharopolyspora erythraea acyl-carrier protein released from recombinant Escherichia coli by freezing and thawing. Biochem. J. 294, 521-527.
15. Gill, S.C. & von Hippel, P.H. (1989). Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182, 319-326.
16. Martin, D.P. & Drueckhammer, D.G. (1992). Combined chemical and enzymatic synthesis of coenzyme A analogs. J. Am. Chem. Soc. 114, 7287-7288.
17. Chiba, T., et al., & Sato, M. (1987). Studies on amino acid derivatives. Part 7. General method for the synthesis of penam and cepham and their substituted derivatives. JCS Perkins / 8, 1845-1851.
18. Shmizu, M., Nagase, O., Hosokawa, Y. & Tagawa, H. (1968). Chemical synthesis of coenzyme A analogs of a modified cysteamine moiety. Tetrahedron 24, 5241-5250.
19. Martin, D.P., Bibart, R.T. & Drueckhammer, D.G. (1994). Synthesis of novel analogs of acetyl coenzyme A: mimics of enzyme reaction intermediates. J. Am. Chem. Soc. 116, 4660-4668.