RNA cleavage without hydrolysis. Splitting the catalytic activities of binase with Asn101 and Thr101 mutations

Protein Engineering

Volumn 10, Issue 3, 1997, Pages 273-278

  Okorokov, Andrei L ^b   Panov, Konstantin I ^a   Offen, Wendy A ^b   Mukhortov, Vladislav G ^a   Antson, Alfred A ^b   Karpeisky, Marat Ya ^a   Wilkinson, Anthony J ^b   Dodson, Guy G ^b  

^a ENGELHARDT INSTITUTE OF MOLECULAR BIOLOGY   (Russian Federation)

^b UNIVERSITY OF YORK   (United Kingdom)

Author keywords

Active site mutants; Binase; Ribonuclease; Site directed mutagenesis

Indexed keywords

ASPARAGINE; BACTERIAL ENZYME; CYCLIC GMP; GLUTAMIC ACID; HISTIDINE; PHOSPHATE; POLYADENYLIC ACID; POLYINOSINIC ACID; RIBONUCLEASE; RNA; THREONINE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; ESTERIFICATION; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; RNA CLEAVAGE; SITE DIRECTED MUTAGENESIS;

BACILLUS INTERMEDIUS; BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 0030935726     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.3.273     Document Type: Article

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