In vitro synthesis of Alternaria allergens and their recognition by murine monoclonal and human IgE antibodies

Annals of Allergy, Asthma and Immunology

Volumn 78, Issue 3, 1997, Pages 287-292

  Bush, Robert K ^a   Sanchez, Hiram ^b  

^a WILLIAM S MIDDLETON MEMORIAL VETERANS HOSPITAL   (United States)

^b UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLERGEN; FUNGAL PROTEIN; IMMUNOGLOBULIN E; IMMUNOGLOBULIN E ANTIBODY; MESSENGER RNA; MONOCLONAL ANTIBODY; POLYADENYLIC ACID;

ALLERGIC REACTION; ALLERGIC RHINITIS; ALTERNARIA; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ASTHMA; CONTROLLED STUDY; DNA LIBRARY; DNA RECOMBINATION; FEMALE; HUMAN; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN SYNTHESIS; RNA TRANSLATION;

EID: 0030934899     PISSN: 10811206     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1081-1206(10)63183-3     Document Type: Article

References (18)

1. Helm RM, Squillace DL, Yunginger JW. Members of the International Collaborative Trial. Production of a proposed international reference standard Alternaria extract, II. Results of a collaborative trial. J Allergy Clin Immunol 1988;81:651-63.
2. Stewart GA. The molecular biology of allergens. In: Busse WW, Holgate SL, eds; Asthma and rhinitis. Cambridge, MA: Blackwell Scientific Publications, USA, 1995:898-932.
3. Arruda LK, Mann BJ, Chapman MD. Selective expression of a major allergen and cytotoxin Asp fI; in Aspergillus fumigatus. J Immunol 1992;149:3354-9.
4. Moser M, Crameri R, Menz G, et al. Cloning and expression of recombinant Aspergillus fumigatus allergen I/a (r AspfI/a) with IgE binding and type 1 skin test activity. J Immunol 1992;149:454-60.
5. Shen HD, Chook B, Lee HH, Hsieh JC, et al. The 40-kilodalton allergen of Candida albicans is an alcohol dehydrogenase: molecular cloning and immunological analysis using monoclonal antibodies. Clin Exp Allergy 1991;21:675-81.
6. Kroutil LA, Bush. RK. Detection of Alternaria allergens by Western blotting. J Allergy Clin Immunol 1987;80:170-6.
7. Stahli C, Staehelin T, Miggiano V, Spleen cell analysis and optimal immunization for high frequency production of specific hybridomas. Methods Enzymol 1983;92:26-36.
8. Meier-Davis S, Folgert J, Bush RK. Partial purification of an Alternaria (ALT) allergen by electroelution. [Abstract], J Allergy Clin Immunol 1988;81:264.
9. Galfre C, Milstein C. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol 1981;73:3-46.
10. Yolken RH, Stopa RJ. Enzyme-linked fluorescent assay: ultrasensitive solidphase assay for detection of human rotavirus. J Clin Microbiol 1979;10:317-31.
11. Chapman MD, Sutherland WM, Platts-Mills TAE. Recognition of two Dermatophagoides pteronyssinus - specific epitopes on antigen P, by using monoclonal antibodies: binding to each epitope can be inhibited by sera from dust mite-allergic patients. J Immunol 1984;133:2488-95.
12. Maniatis T, Fritch EF, Sanbrook J, eds. Molecular cloning. A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1982: 196-8.
13. + RNA, In Berge SL, Kimmel AR, eds. Guide to molecular cloning techniques. Methods Enzymol 1987;152:254-61.
14. Maniatis T, Fritch EF, Sanbrook J, eds. Molecular cloning. A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1982;202-3.
15. Promega Protocols and Application Guide. Promega Corp., Madison, WI, 1989.
16. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350-4.
17. Breiteneder H, Hassfeld W, Petten-burger K, et al. Isolation and characterization of messenger RNA from male inflorescences and pollen of the white birch (Betula verrucosa). Int Arch Appl Immunol 1988;87:19-24.
18. Yunginger JW, Jones RT, Nesheim ME, Geller M. Studies on Alternaria allergens. III. Isolation of a major allergenic fraction (ALT-1). J Allergy Clin Immunol 1980;66:138-47.