In vivo commitment to splicing in yeast involves the nucleotide upstream from the branch site conserved sequence and the Mud2 protein

EMBO Journal

Volumn 16, Issue 7, 1997, Pages 1759-1771

  Rain, Jean Christophe ^a   Legrain, Pierre ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

galactosidase; Nuclear export; Pre mRNA; S.cerevisiae; Spliceosome

Indexed keywords

FUNGAL PROTEIN; MESSENGER RNA PRECURSOR; SMALL NUCLEAR RNA;

ARTICLE; GENETIC CONSERVATION; INTRON; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; RNA SPLICING; YEAST;

BASE COMPOSITION; BASE SEQUENCE; BETA-GALACTOSIDASE; CONSERVED SEQUENCE; FUNGAL PROTEINS; GENES, FUNGAL; GENES, REPORTER; MUTAGENESIS, SITE-DIRECTED; OLIGODEOXYRIBONUCLEOTIDES; OPEN READING FRAMES; POINT MUTATION; RECOMBINANT FUSION PROTEINS; REGULATORY SEQUENCES, NUCLEIC ACID; RIBONUCLEOPROTEINS; RNA PRECURSORS; RNA SPLICING; RNA, FUNGAL; RNA, MESSENGER; RNA, SMALL NUCLEAR; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0030934772     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.7.1759     Document Type: Article

References (54)

1. Abovich,N., Liao,X.C. and Rosbash,M. (1994) The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition. Genes Dev., 8, 843-854.
2. Ares,M.J. and Weiser,B. (1995) Rearrangement of snRNA structure during assembly and function of the spliceosome. Prog. Nucleic Acid Res. Mol. Biol., 50, 131-159.
3. Arning,S., Grüter,P., Bilbe,G. and Krämer,A. (1996) Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA. RNA, 2, 794-810.
4. Baldari,C. and Cesareni,G. (1985) Plasmids pEMBLY: new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences. Gene, 35, 27-32.
5. Behrens,S.E., Galisson,F., Legrain,P. and Lührmann,R. (1993) Evidence that the 60-kDa protein of 17S U2 small nuclear ribonucleoprotein is immunologically and functionally related to the yeast PRP9 splicing factor and is required for the efficient formation of prespliceosomes. Proc. Natl Acad. Sci. USA, 90, 8229-8233.
6. Bennett,M. and Reed,R. (1993) Correspondence between a mammalian spliceosome component and an essential yeast splicing factor. Science, 262, 105-108.
7. Bonneaud,N., Ozier-Kalogeropoulos,O., Li,G.Y., Labouesse,M., Minvielle-Sebastia,L. and Lacroute,F. (1991) A family of low and high copy replicative, integrative and single-stranded S.cerevisiae/E. coli shuttle vectors. Yeast, 7, 609-615.
8. Brosi,R., Gröning,K., Behrens,S.E., Lührmann,R. and Krämer,A. (1993a) Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9. Science, 262, 102-105.
9. Brosi,R., Hauri,H.P. and Krämer,A. (1993b) Separation of splicing factor SF3 into two components and purification of SF3a activity. J. Biol. Chem., 268, 17640-17646.
10. Burgess,S.M. and Guthrie,C. (1993) A mechanism to enhance mRNA splicing fidelity: the RNA-dependent ATPase Prp16 governs usage of a discard pathway for aberrant lariat intermediates. Cell, 73, 1377-1391.
11. Burgess,S., Couto,J.R. and Guthrie,C. (1990) A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing. Cell, 60, 705-717.
12. Chanfreau,G., Legrain,P., Dujon,B. and Jacquier,A. (1994) Interaction between the first and last nucleotides of pre-mRNA introns is a determinant of 3′ splice site selection in S.cerevisiae. Nucleic Acids Res., 22, 1981-1987.
13. Chapon,C. and Legrain,P. (1992) A novel gene, spp91-1, suppresses the splicing defect and the pre-messenger-RNA nuclear export in the prp9-1 mutant. EMBO J., 11, 3279-3288.
14. Colot,H.V., Stutz,F. and Rosbash,M. (1996) The yeast splicing factor Mud13 is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex. Genes Dev., 10, 1699-1708.
15. Crispino,J.D., Blencowe,B.B. and Sharp,P.A. (1994) Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP. Science, 265, 1866-1869.
16. Fouser,L.A. and Friesen,J.D. (1986) Mutations in a yeast intron demonstrate the importance of specific conserved nucleotides for the two stages of nuclear mRNA splicing. Cell, 45, 81-93.
17. Fouser,L.A. and Friesen,J.D. (1987) Effects on mRNA splicing of mutations in the 3′ region of the Soccharomyces cerevisiae actin intron. Mol. Cell. Biol., 7, 225-230.
18. Fu,X.D. (1995) The superfamily of arginine/serine-rich splicing factors. RNA, 1, 663-680.
19. Gozani,O., Patton,J.G. and Reed,R. (1994) A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction. EMBO J., 13, 3356-3367.
20. Gozani,O., Feld,R. and Reed,R. (1996) Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A. Genes Dev., 10, 233-243.
21. Guarente,L., Yocum,R. and Gifford,P. (1982) A GAL10-CYC1 hybrid yeast promoter identifies the Ga14 regulatory region as an upstream site. Proc. Natl Acad. Sci. USA, 79, 7410-7414.
22. Hodges,P.E. and Beggs,J.D. (1994) U2 fulfils a commitment. Curr. Biol., 4, 264-267.
23. Jacquier,A., Rodriguez,J.R. and Rosbash,M. (1985) A quantitative analysis of the effects of 5′ junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing. Cell, 43, 423-430.
24. Krämer,A. (1996) The structure and function of proteins involved in mammalian pre-mRNA splicing. Annu. Rev. Biochem., 65, 367-409.
25. Krämer,A., Legrain,P., Mulhauser,F., Gröning,K., Brosi,R. and Bilbe,G. (1994) Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo. Nucleic Acids Res., 22, 5223-5228.
26. Krämer,A., Mulhauser,F., Wersing,C., Gröning,K. and Bilbe,G. (1995) Mammmalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae. RNA, 1, 260-272.
27. Legrain,P. and Chapon,C. (1993) Interaction between PRP11 and SPP91 yeast splicing factors and characterization of a PRP9-PRP11-SPP91 complex. Science, 262, 108-110.
28. Legrain,P. and Rosbash,M. (1989) Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Cell, 57, 573-583.
29. Legrain,P., Séraphin,B. and Rosbash,M. (1988) Early commitment of yeast pre-mRNA to the spliceosome pathway. Mol. Cell. Biol., 8, 3755-3760.
30. Liao,X.C., Tang,J. and Rosbash,M. (1993) An enhancer screen identifies a gene that encodes the yeast U1 snRNP A protein: implications for snRNP protein function in pre-mRNA splicing. Genes Dev., 7, 419-428.
31. Long,R.M., Elliott,D.J., Stutz,F., Rosbash,M. and Singer,R.H. (1995) Spatial consequences of defective processing of specific yeast mRNAs revealed by fluorescent in situ hybridization. RNA, 1, 1071-1078.
32. MacMillan,A.M., Query,C.C., Allerson,C.R., Chen,S., Verdine,G.L. and Sharp,P.A. (1994) Dynamic association of proteins with the pre-mRNA branch region. Genes Dev., 8, 3008-3020.
33. Madhani,H.D. and Guthrie,C. (1994) Dynamic RNA-RNA interactions in the spliceosome. Annu. Rev. Genet., 28, 1-26.
34. Michaud,S. and Reed,R. (1991) An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway. Genes Dev., 5, 2534-2546
35. Michaud,S. and Reed,R. (1993) A functional association between the 5′ and 3′ splice site is established in the earliest prespliceosome complex (E) in mammals. Genes Dev., 7, 1008-1020.
36. Parker,R. and Guthrie,C. (1985) A point mutation in the conserved hexanucleotide at a yeast 5′ splice junction uncouples recognition, cleavage, and ligation. Cell, 41, 107-118.
37. Parker,R., Siliciano,P.G. and Guthrie,C. (1987) Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA. Cell, 49, 229-239.
38. Pikielny,C.W. and Rosbash,M. (1985) mRNA splicing efficiency in yeast and the contribution of nonconserved sequences. Cell, 41, 119-126.
39. Pikielny,C.W. and Rosbash,M. (1986) Specific small nuclear RNAs are associated with yeast spliceosomes. Cell, 45, 869-877.
40. Rain,J.-C., Tartakoff,A.M., Krämer,A. and Legrain,P. (1996) Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution. RNA, 2, 535-550.
41. Rosbash,M. and Séraphin,B. (1991) Who's on first? The U1 snRNP-5′ splice site interaction and splicing. Trends Biochem. Sci., 16, 187-190.
42. Rothstein,R.J. (1983) One-step gene disruption in yeast. Methods Enzymol., 101, 202-211.
43. Ruby,S.W., Chang,T.H. and Abelson,J. (1993) Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA. Genes Dev., 7, 1909-1925.
44. Rymond,B.C. and Rosbash,M. (1985) Cleavage of 5′ splice site and lariat formation are independent of 3′ splice site in yeast mRNA splicing. Nature, 317, 735-737.
45. Séraphin,B. and Rosbash,M. (1989) Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing. Cell, 59, 349-358.
46. Séraphin,B. and Rosbash,M. (1990) Exon mutations uncouple 5′ splice site selection from U1 snRNA pairing. Cell, 63, 619-629.
47. Séraphin,B. and Rosbash,M. (1991) The yeast branchpoint sequence is not required for the formation of a stable U1 snRNA-pre-mRNA complex and is recognized in the absence of U2 snRNA. EMBO J., 10, 1209-1216.
48. Smith,V. and Barrell,B. (1991) Cloning of a yeast U1 snRNP 70K protein homologue: functional conservation of RNA-binding domain between humans and yeast. EMBO J., 10, 2627-2634.
49. Tarn,W.-H. and Steitz,J.A. (1994) SR proteins can compensate for the loss of U1 snRNP functions in vitro. Genes Dev., 8, 2704-2717.
50. Transy,C. and Legrain,P. (1995) The two-hybrid: an in vivo protein-protein interaction assay. Mol. Biol. Rep., 21, 119-127.
51. 65 RS region with pre-mRNA and promotion of branch point base pairing with U2 snRNA. Science, 273, 1706-1709.
52. Vijayraghavan,U., Parker,R., Tamm,J., Iimura,Y., Rossi,J., Abelson,J. and Guthrie,C. (1986) Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome. EMBO J., 5, 1683-1695.
53. Wells,S.E. and Ares,M.J. (1994) Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae. Mol. Cell. Biol., 14, 6337-6349.
54. Wells,S.E., Neville,M., Haynes,M., Wang,J., Igel,H. and Ares,M.J. (1996) CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145. Genes Dev., 10, 220-232.