A highly efficient and stable system for site-specific integration of genes and plasmids into the phage ∅LC3 attachment site (attB) of the Lactococcus lactis chromosome

Gene

Volumn 188, Issue 1, 1997, Pages 129-136

  Lillehaug, Dag ^a   Nes, Ingolf F ^a   Birkeland, Nils Kåre ^a,b  

^a NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

^b UNIVERSITY OF BERGEN   (Norway)

Author keywords

Chromosomal targeting; Genetic stabilization; Integration vector; Temperate bacteriophage

Indexed keywords

INTEGRASE;

ARTICLE; BACTERIOPHAGE; CHROMOSOME; CLONING VECTOR; DNA INTEGRATION; GENE CASSETTE; GENETIC TRANSFORMATION; LACTOCOCCUS; LACTOCOCCUS LACTIS; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE;

BACTERIOPHAGES; CHROMOSOMES, BACTERIAL; DNA REPLICATION; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; LACTOCOCCUS LACTIS; MUTAGENESIS, SITE-DIRECTED; VIRUS INTEGRATION;

LACTOCOCCUS; LACTOCOCCUS LACTIS; LACTOCOCCUS LACTIS SUBSP. LACTIS; STREPTOCOCCUS;

EID: 0030933511     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00798-6     Document Type: Article

References (33)

1. Atlung, T., Nielsen, A., Rasmussen, L.J., Nellemann, L.J. and Holm, F. (1991) A versatile method for integration of genes and gene fusions into the λ attachment site of Escherichia coli. Gene 107, 11-17.
2. Biswas, I., Gruss, A., Ehrlich, S.D. and Maguin, E. (1993) High-efficiency gene inactivation and replacement system for Gram-positive bacteria. J. Bacteriol. 175, 3628-3635.
3. Chopin, M.-C., Chopin, A., Rouault, A. and Galleron, N. (1989) Insertion and amplification of foreign genes in the Lactococcus lactis subsp. lactis chromosome. Appl. Environ. Microbiol. 55, 1769-1774.
4. Christiansen, B., Johnsen, M.G., Vogensen, F.K. and Hammer, K. (1994) Characterization of the lactococcal temperate phage TP901-1 and its site-specific integration. J. Bacteriol. 176, 1069-1076.
5. Church, G.M. and Gilbert, W. (1984) Genomic sequencing. Proc. Natl. Acad. Sci. USA 81, 1991-1995.
6. Davies, F.L. and Gasson, M.J. (1981) Reviews of the progress of dairy science: genetics of lactic acid bacteria. J. Dairy Res. 48, 363-376.
7. Efstathiou, J.D. and McKay, L.L. (1977) Inorganic salts resistance associated with a lactose-fermenting plasmid in Streptococcus lactis. J. Bacteriol. 130, 257-265.
8. Fu, J.F., Chang, R.-Y. and Tseng, Y.-H. (1992) Construction of stable lactose-utilizing Xanthomonas campestris by chromosomal integration of cloned lac genes using filamentous φLf DNA. Appl. Microbiol Biotechnol. 37, 225-229.
9. Gottesman, M.E. and Weisberg, R.A. (1971) Prophage insertion and excision. In: Hershey, A.D. (Ed.), The Bacteriophage Lambda. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 113-138.
10. Hermesz, E., Olasz, F., Dorgai, L. and Orosz, L. (1992) Stable incorporation of genetic material into the chromosome of Rhizobium meliloti 41: construction of an integrative vector system. Gene 119, 9-15.
11. Higgins, C.F., Dorman, C.J., Stirling, D.A., Waddell, L., Booth, I.R., May, G. and Bremer, E. (1988) A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli. Cell 52, 569-584.
12. Holo, H. and Nes, I.F. (1989) High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl. Environ. Microbiol. 55, 3119-3123.
13. Kuhstoss, S., M., Richardson, A. and Rao, R.N. (1991) Plasmid cloning vectors that integrate site-specifically in Streptomyces spp. Gene 97, 143-146.
14. Lee, C.Y., Buranen, S.L. and Ye., Z.-H. (1991a) Construction of single-copy integration vectors for Staphylococcus aureus. Gene 103, 101-105.
15. Lee, M.H., Pascopella, L., Jacobs, W.R. Jr. and Hatfull, G.F. (1991b) Site-specific integration of mycobacteriophage L5: Integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and Bacille Calmette-Guèrin. Proc. Natl. Acad. Sci. USA 88, 3111-3115.
16. Leenhouts, K.J., Kok, J. and Venema, G. (1989) Campbell-like integration of heterologous plasmid DNA into the chromosome of Lactococcus lactis subsp. lactis. Appl. Environ. Microbiol. 55, 394-400.
17. Lillehaug, D. and Birkeland, N.K. (1993) Characterization of genetic elements required for site-specific integration of the temperate lactococcal bacteriophage φLC3 and construction of integration-negative φLC3 mutants. J. Bacteriol. 175, 1745-1755.
18. Lillehaug, D., Lindqvist, B.H. and Birkeland, N.K. (1991) Characterization of φLC3, a Lactococcus lactis subsp. cremoris temperate bacteriophage with cohesive single-stranded DNA ends. Appl. Environ. Microbiol. 57, 3206-3211.
19. Maguin, E., Duwat, P., Hege, T., Ehrlich, S.D. and Gruss, A. (1992) New thermosensitive plasmid for Gram-positive bacteria. J. Bacteriol. 174, 5633-5638.
20. McIntyre, D.A. and Harlander, S.K. (1993) Construction of first-generation lactococcal integrative cloning vectors. Appl. Microbiol. Biotechnol. 40, 348-355.
21. McKay, L.L. (1983) Functional properties of plasmids in lactic streptococci. Antonie van Leeuwenhoek J. Microbiol. 49, 259-274.
22. McKay, L.L. and Baldwin, K.A. (1990) Applications for biotechnology: present and future improvements in lactic acid bacteria. FEMS Microbiol. Rev. 87, 3-14.
23. Mizuuchi, K., Gellert, M. and Nash, H. (1978) Involvement of supertwisted DNA in integrative recombination of bacteriophage lambda. J. Mol. Biol. 121, 375-392.
24. Noirot, P., Petit, M.-A. and Ehrlich, S.D. (1987) Plasmid replication stimulates DNA recombination in Bacillus subtilis. J. Mol. Biol. 196, 39-48.
25. Raya, R.R., Fremaux, C., de Antoni, G.L. and Klaenhammer, T.R. (1992) Site-specific integration of the temperate bacteriophage φadh into the Lactobacillus gasseri chromosome and molecular characterization of the phage (attP) and bacterial (attB) attachment sites. J. Bacteriol. 174, 5584-5592.
26. Romero, D.A. and Klaenhammer, T.R. (1992) IS946-mediated integration of heterologous DNA into the genome of Lactococcus lactis subsp. lactis. Appl. Environ. Microbiol. 58, 699-702.
27. Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S., Russel, H., Horn, G.T., Mullis, K.B. and Ehrlich, H. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-491.
28. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn., Vol. 1-3. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
29. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5767.
30. Terzaghi, B.E. and Sandine W.E. (1975) Improved medium for lactic streptococci and their bacteriophages. Appl. Environ. Microbiol. 29, 807-813.
31. van de Guchte, M., Daly, C., Fitzgerald, G.F. and Arendt, E.K. (1994) Identification of int and attP on the genome of lactococcal bacteriophage Tuc2009 and their use for site-specific plasmid integration in the chromosome of Tuc2009-resistant Lactococcus lactis MG1363. Appl. Environ. Microbiol. 60, 2324-2329.
32. von Wright, A., Tynkkinen, S. and Suominen, M. (1987) Cloning of a Streptococcus lactis chromosomal fragment associated with the ability to grow in milk. Appl. Environ. Microbiol. 53, 584-588.
33. Young, M. and Ehrlich, S.D. (1989) Stability of reiterated sequences in the Bacillus subtilis chromosome. J. Bacteriol. 171, 2653-2656.