The ordered phosphorylation of cardiac troponin I by the cAMP-dependent protein kinase: Structural consequences and functional implications

European Journal of Biochemistry

Volumn 248, Issue 2, 1997, Pages 329-337

  Keane, Noeleen E ^a   Quirk, Philip G ^a   Gao, Yuan ^a   Patchell, Valerie B ^a   Perry, S Victor ^a   Levine, Barry A ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Electrophoresis; NMR; Phosphorylation; Troponin C; Troponin I

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; TROPONIN I;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; GEL ELECTROPHORESIS; HEART MUSCLE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; REACTION ANALYSIS;

EID: 0030931106     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00329.x     Document Type: Article

References (23)

1. Al Hillawi, E., Bhandari, D. G., Trayer, H. R. & Trayer, I. P. (1995) The effects of phosphorylation of cardiac troponin-I on its interactions with actin and cardiac troponin-C, Eur. J. Biochem. 228, 962-970.
2. 2+-stimulated ATPase of skeletal muscle actomyosin, FEBS Lett. 72, 162-169.
3. Barsukov, I. L. & Lian, L-U. (1993) Structure determination from NMR data I. Analysis of NMR data, in NMR of Macromolecules, a Practical Approach (Roberts, G. C. K., ed.) pp. 317-357, Oxford University Press, Oxford.
4. Dalgarno, D. C., Grand, R. J. A., Levine, B. A, Moir, A. J. G., Scott, G. M. M. & Perry, S. V. (1982) Interaction between troponin I and troponin C. Definition of topography by proton magnetic resonance spectroscopy, FEBS Lett. 150, 54-58.
5. Edelman, A. M., Blumenthal, D. K. & Krebs, E. G. (1987) Protein serine/threonine kinases, Annu. Rev. Biochem. 56, 567-613.
6. Farah, C. S. & Reinach, F. C. (1995) Troponin and muscular contraction, FASEB J. 9, 755-767.
7. 1H NMR spectroscopy, Biochem. Biophys. Acta 1160, 22-34.
8. Grand, R. J. A. & Perry, S. V. (1980) The binding of calmodulin to myelin basic protein and histone H2B, Biochem. J. 189, 227-240.
9. Head, J. P. & Perry, S. V. (1974) The interaction of the calcium binding protein (troponin C) with bivalent cations and the inhibitory protein (troponin I), Biochem. J. 137, 145-154.
10. Knighton, D. R., Zheng, J., Ten Eyck, L. F., Xuong, N.-H., Taylor, S. S. & Sowadski, J. M. (1991) Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependant protein kinase, Science 253, 414-420.
11. 1H chemical-shifts obtained as a function of temperature and trifluoroethanol concentration for the peptide series GGXGG, J. Biomol. NMR 5, 14-24.
12. Mittmann, K., Jaquet, K. & Heilmeyer, L. M. G. (1992) Ordered phosphorylation of a minimal recognition motif for cAMP dependent protein kinase present in cardiac troponin I, FEBS Lett. 302, 133-137.
13. Moir, A. J. G., Solaro, J. & Perry, S. V. (1980) The site of phosphorylation of troponin I in the perfused heart: the effect of adrenaline, Biochem. J. 185, 505-513.
14. O'Neil, K. T. & De Grado, W. F. (1990) How calmodulin binds its targets: sequence independent recognition of amphiphilic α-helices, Trends Biochem. Sci. 15, 59-64.
15. Pardi, A., Wagner, G. & Wüthrich, K. (1993) Protein conformation and proton NMR chemical-shifts, Eur. J. Biochem. 137, 445-454.
16. Quirk, P. G., Patchell, V. B., Gao, Y., Levine, B. A. & Perry S. V. (1995) Sequential phosphorylation of adjacent serine residues on the N-terminal region of troponin I: structure function implications of ordered phosphorylation, FEBS Lett. 370, 175-178.
17. Ray, K. P. & England, P. J. (1976) Phosphorylation of the inhibitory subunit of troponin and its effect on the calcium dependence of the cardiac myofibril adenosine triphosphatase, FEBS Lett. 70, 11-16.
18. 2+-regulatory site of bovine cardiac troponin, J. Biol. Chem. 257, 260-263.
19. Solaro, J., Moir, A. J. G. & Perry, S. V. (1976) Phosphorylation of troponin I and the ionotropic effect of adrenaline in the perfused rabbit heart, Nature 262, 615-617.
20. Smith, L. J., Bolin, K. A., Schwalbe, H., Macarthur, M. W., Thornton, J. M. & Dobson, C. M. (1996) Analysis of main-chain torsion angles in proteins - prediction of NMR coupling-constants for native and random coil conformations, J. Mol. Biol. 255, 494-506.
21. 31P-NMR spectroscopy and phosphorylation by protein kinases, Eur. J. Biochem. 190, 575-582.
22. 15N random coil NMR chemical-shifts of the common amino-acids. 1. investigations of nearest-neighbour effects, J. Biomol. NMR 5, 67-81.
23. 2+ affinity of cardiac troponin C, J. Biol. Chem. 270, 30773-30780.