Sperm separation for gender preference: Methods and efficacy

Journal of Andrology

Volumn 18, Issue 2, 1997, Pages 107-108

  Botchan, Amnon ^a,b   Hauser, Ron ^a   Gamzu, Roni ^a   Yogev, Leah ^a   Paz, Gedalja ^a   Yavetz, Haim ^a  

^a TEL AVIV UNIVERSITY   (Israel)

^b Serlin Maternity Hospital   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ARTIFICIAL INSEMINATION; CELL SELECTION; CELL SEPARATION; FLOW CYTOMETRY; GENDER; HUMAN; KARYOTYPING; MALE; NONHUMAN; PRIORITY JOURNAL; SEX DETERMINATION; SEX RATIO; SHORT SURVEY; SPERM; X CHROMOSOME; Y CHROMOSOME;

CELL SEPARATION; FEMALE; HUMANS; KARYOTYPING; MALE; SEX DETERMINATION (ANALYSIS); SEX PRESELECTION; SEX RATIO; SPERMATOZOA; X CHROMOSOME; Y CHROMOSOME;

EID: 0030926521     PISSN: 01963635     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Short Survey

References (20)

1. Barlow P, Vosa CG. The Y chromosome in human spermatozoa. Nature 1970;226:961-962.
2. Beatty RA. F-bodies as chromosomes markers in mature human sperm heads. Cytogenet Cell Gener 1977;18:33-49.
3. Bradley MP. Immunological sexing of mammalian semen: current status and future options. J Dairy Sci 1989;72:3372-3380.
4. Engelmann U, Rassrigg F, Schatz H, Schill WB. Separation of human X and Y spermatozoa by free-flow eleetrophoresis. Gamete Res 1988;19:151-159.
5. Ericsson RJ, Langevin CN, Nishino M. Isolation of fractions rich in human Y sperm. Nature 1973;246:421-424.
6. Gordon K, Brown DB, Ruddle FH. In vitro activation of human sperm induced by amphibian egg extract. Exp Cell Res 1985;157:409-418.
7. Ishijima SA, Okuno M, Nahori Y, Seki S, Nagafuchi S, Kaneko S, Mohri H. Separation of X- and Y-chromosome-bearing murine sperm by free-flow eleetrophoresis: evaluation of separation using PCR. Zool Sci 1992;9:601-606.
8. Johnson LA. Gender preselection in domestic animals using flow cytometrically sorted sperm. J Anim Sci 1992;70:8-18.
9. Johnson LA, Clarke RN. Flow sorting of X and Y chromosome-bearing mammalian sperm: activation and pronuclear development of sorted bull, boar and ram sperm microinjected into hamster oocytes. Gamete Res 1988;21:335-343.
10. Johnson LA, Schulman JD. Human sperm selection. The safety of sperm selection by flow cytometry. Hum Reprod 1994;9:758-759.
11. Johnson LA, Welch GR, Keyvanfar K, Dorfmann A, Fugger EF, Schulman JD. Gender preselection in humans? Flow cytometric separation of X and Y spermatozoa for the prevention of X-linked diseases. Hum Reprod 1993;8:1733-1739.
12. Levinson G, Keyvanfar K, Wu JC, Fugger EF, Fields RA, Harton GL, Palmer FT, Sisson ME, Starr KM, Dennison-Lagos L, Calvo L, Sherins RJ, Bick D, Schulman JD, Black SH. DNA-based X-enriched sperm separation as an adjunct to preimplantation genetic testing for the prevention of X-linked disease. Hum Reprod 1995;10:979-982.
13. Morrell JM, Dresser DW. Offspring from inseminations with mammalian sperm stained with Hoeschst 33342, either with or without flow cytometry. Mutat Res 1989;224:177-183.
14. Perreault SD, Zirkin BR. Sperm nuclear decondensation in mammals: role of sperm associated proteinase in vivo. J Exp Zool 1982;224:253-257.
15. Rudak E, Jacobs PA, Yanagimachi H. Direct analysis of the chromosome constitution of human spermatozoa. Nature 1978;274:911-913.
16. Shettles LB. Factors influencing sex ratios. Int J Gynaecol Obstet 1970;8:643-647.
17. Steeno O, Adimoelja A, Steeno J. Separation of X and Y bearing human spermatozoa with the sephadex gel-filtration method. Andrologia 1975;7:95.
18. van Kooij RJ, van Oost BA. Determination of sex ratio of spermatozoa with a deoxyribonucleic acid-probe and quinaerine staining: a comparison. Fertil Steril 1992;58:384-386.
19. Vidal F, Moragas M, Catala V, Torello MJ, Santalo J, Calderon G, Gimenez C, Barri PN, Egozcue J, Veiga A. Sephadex filtration and human serum albumin gradients do not select spermatozoa by sex chromosome: a fluorescent in-situ hybridization study. Hum Reprod 1993;8:1740-1743.
20. Yanagimachi R, Yanagimachi H, Rogers BJ. The use of zona-free animal ova as a test-system for the assessment of the fertilizing capacity of human spermatozoa. Biol Reprod 1976;15:471-476.