A novel resistance mechanism against β-lactams in Streptococcus pneumoniae involves CpoA, a putative glycosyltransferase

Journal of Bacteriology

Volumn 179, Issue 10, 1997, Pages 3342-3349

  Grebe, Thorsten ^a,b   Paik, Johanna ^a   Hakenbeck, Regine ^a  

^a MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^b PRINCETON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL ENZYME; BETA LACTAM ANTIBIOTIC; ERYTHROMYCIN; GLYCOSYLTRANSFERASE; PENICILLIN DERIVATIVE; PIPERACILLIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL CELL WALL; BACTERIAL MEMBRANE; BACTERIUM MUTANT; BIOSYNTHESIS; DNA SEQUENCE; ENZYME ACTIVITY; GENE LOCUS; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PENICILLIN RESISTANCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STREPTOCOCCUS PNEUMONIAE;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); EUKARYOTA; NEGIBACTERIA; PROKARYOTA; STREPTOCOCCUS PNEUMONIAE;

EID: 0030924861     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.10.3342-3349.1997     Document Type: Article

References (53)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Avery, O. T., C. M. MacLeod, and M. McCarty. 1944. Studies on the chemical nature of the substance inducing transformation of pneumococcal types. J. Exp. Med. 79:137-158.
3. Blake, M. S., K. H. Johnson, G. Russell-Jones, and E. C. Gotschlich. 1984. A rapid sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal. Biochem. 136:175-179.
4. Chen, J.-D., and D, A, Morrison. 1988. Construction and properties of a new insertion vector, pJDC9, that is protected by transcriptional terminators and useful for cloning of DNA from Streptococcus pneumoniae. Gene 64:155-164.
5. Colman, G. 1968. The application of computers to the classification of streptococci. J. Gen. Microbiol. 50:149-196.
6. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
7. Farrell, R. E. J. 1993. RNA methodologies: a laboratory guide for isolation and characterization. Academic Press, Inc., San Diego, Calif.
8. Fassler, J. S., W. Gray, J. P. Lee, G. Yu, and G. Gingerich. 1991. The Saccharomyces cerevisiae SPT14 gene is essential for normal expression of the yeast transposon, Ty, as well as for expression of the HIS4 gene and several genes in the mating pathway. Mol. Gen. Genet. 230:310-320.
9. Ghuysen, J.-M., and G. Dive. 1994. Biochemistry of the penicilloyl-serine transferases, p. 103-129. In J.-M. Ghuysen and R. Hakenbeck (ed.), Bacterial ceil wall. Elsevier Sciences BV, Amsterdam, The Netherlands.
10. Grebe, T. 1995. Eine neue Resistenzdeterminante in Piperacillin-resistenten Labormutanten von Streptococcus pneumoniae. Dissertation. Freie Universität Berlin, Berlin, Germany.
11. Grebe, T., and R. Hakenbeck. 1996. Penicillin-binding proteins 2b and 2x of Streptococcus pneumoniae are primary resistance determinants for different classes of β-lactam antibiotics. Antimicrob. Agents Chemother. 40:829-834.
12. Guenzi, E., A. M. Gasc, M. A. Sicard, and R. Hakenbeck. 1994. A two-component signal-transducing system is involved in competence and penicillin susceptibility in laboratory mutants of Streptococcus pneumoniae. Mol. Microbiol. 12:505-515.
13. Hakenbeck, R. 1995. Target mediated resistance to β-lactam antibiotics. Biochem. Pharmacol. 50:1121-1127.
14. Hakenbeck, R., H. Ellerbrok, and T. Briese. 1986. Release of penicillin-binding proteins from β-lactam treated bacteria: determination by anti-β-lactam antibodies, p. 83-88. In P. H. Seidl and K. H. Schleifer (ed.), Biological properties of peptidoglycan. Walter de Gruyter & Co., Berlin, Germany.
15. Hakenbeck, R., H. Ellerbrok, T. Briese, S. Handwerger, and A. Tomasz. 1986. Penicillin-binding proteins of penicillin-susceptible and -resistant pneumococci: immunological relatedness of altered proteins and changes in peptides carrying the β-lactam binding site. Antimicrob. Agents Chemother. 30:553-558.
16. Hakenbeck, R., T. Grebe, E. Guenzi, A. König, J. Krauß, and P. Reichmann. 1995. The role of penicillin-binding proteins in penicillin resistance of Streptococcus pneumoniae, p. 115-123. In J. J. Ferretti, M. S. Gilmore, T. R. Klaenhammer, and F. Brown (ed.), Genetics of streptococci, enterococci and lactococci. S. Karger AG, Basel, Switzerland.
17. Hakenbeck, R., C. Martin, C. Dowson, and T. Grebe. 1994. Penicillin-binding protein 2b of Streptococcus pneumoniae in piperacillin-resistant laboratory mutants. J. Bacteriol. 176:5574-5577.
18. Hakenbeck, R., S. Tornette, and N. F. Adkinson. 1987. Interaction of non-lytic β-lactams with penicillin-binding proteins in Streptococcus pneumoniae. J. Gen. Microbiol. 133:755-760.
19. Hakenbeck, R., S. Waks, and A. Tomasz. 1978. Characterization of cell wall polymers secreted into the growth medium of lysis-defective pneumococci during treatment with penicillin and other inhibitors of cell wall synthesis. Antimicrob. Agents Chemother. 13:302-311.
20. Håvarstein, L. S., G. Coomaraswamy, and D. A. Morrison. 1995. An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae. Proc. Natl. Acad. Sci. USA 92:11140-11144.
21. Håvarstein, L. S., H. Holo, and I. F. Nes. 1994. The leader peptide of colicin V shares consensus sequences with leader peptides that are common among peptide bacteriocins produced by gram-positive bacteria. Microbiology 140: 2383-2389.
22. Honeyman, A. L., and G. C. Stewart. 1989. The nucleotide sequence of the rodC operon of Bacillus subtilis. Mol. Microbiol. 3:1257-1268.
23. Hui, F. M., and D. A. Morrison. 1991. Genetic transformation in Streptococcus pneumoniae: nucleotide sequence analysis shows comA, a gene required for competence induction, to be a member of the bacterial ATP-dependent transport protein family. J. Bacteriol. 173:372-381.
24. Johnston, T. C., R. B. Thompson, and T. O. Baldwin. 1986. Nucleotide sequence of the luxB gene of Vibrio harveyi and the complete amino acid sequence of the beta subunit of bacterial luciferase. J. Biol. Chem. 261:4805-4811.
25. 24a. Krauß, J., and R. Hakenbeck. Unpublished results.
26. Krauß, J., M. van der Linden, T. Grebe, and R. Hakenbeck. 1996. Penicillin-binding proteins 2x and 2b as primary PBP-targets in Streptococcus pneumoniae. Microb. Drug Res. 2:183-186.
27. Lacks, S. A., and R. D. Hotchkiss. 1960. A study of the genetic material determining an enzyme activity in pneumococcus. Biochim. Biophys. Acta 39:508-517.
28. Laible, G., and R. Hakenbeck. 1987. Penicillin-binding proteins in β-lactam-resistant laboratory mutants of Streptococcus pneumoniae. Mol. Microbiol. 1:355-363.
29. Laible, G., and R. Hakenbeck. 1991. Five independent combinations of mutations can result in low-affinity penicillin-binding protein 2x of Streptococcus pneumoniae. J. Bacteriol. 173:6986-6990.
30. Laible, G., R. Hakenbeck, M. A. Sicard, B. Joris, and J.-M. Ghuysen. 1989. Nucleotide sequences of the pbpX genes encoding the penicillin-binding protein 2x from Streptococcus pneumonias R6 and a cefotaxime-resistant mutant, C506. Mol. Microbiol. 3:1337-1348.
31. Lazarevic, V., and D. Karamata. 1995. The tagGH operon of Bacillus subtilis 168 encodes a two-component ABC transporter involved in the metabolism of two wall teichoic acids. Mol. Microbiol. 16:345-355.
32. Livermore, D. M. 1995. β-Lactamases in laboratory and clinical resistance. Clin. Microbiol. Rev. 8:557-584.
33. Lonetto, M. A., K. L. Brown, K. E. Rudd, and M. J. Buttner. 1994. Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase a factors involved in the regulation of extracytoplasmic functions. Proc. Natl. Acad. Sci. USA 91:7573-7577.
34. Maina, C. V., P. D. Riggs, A. G. I. Grandea, B. E. Slatko, L. S. Moran, J. A. Tagliamonte, L. A. McReynolds, and C. Guan. 1988. A vector to express and purify foreign protein in Escherichia coli by fusion to, and separation from, maltose binding protein. Gene 74:365-373.
35. Martin, B., P. García, M.-P. Castanié, and J.-P. Claverys. 1995. The recA gene of Streptococcus pneumoniae is part of a competence-induced operon and controls lysogenic induction. Mol. Microbiol. 15:367-379.
36. Martin, C., T. Briese, and R. Hakenbeck. 1992. Nucleotide sequences of genes encoding penicillin-binding proteins from Streptococcus pneumoniae and Streptococcus oralis with high homology to Escherichia coli penicillin-binding proteins 1A and 1B. J. Bacteriol. 174:4517-4523.
37. Martinez, E., B. Bartolomé, and F. de la Cruz. 1988. pACYC184-derived cloning vectors containing the multiple cloning site and lacZα reporter gene of pUC8/9 and pUC18/19 plasmids. Gene 68:159-162.
38. Messing, J., R. Crea, and P. H. Seeburg. 1981. A system for shotgun DNA sequencing. Nucleic Acids Res. 9:309-321.
39. Miyata, T., J. Takeda, Y. Iida, N. Yamada, N. Inoue, M. Takahashi, K. Maeda, T. Kitani, and T. Kinoshita. 1993. The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis. Science 259:1318-1320.
40. Nikaido, H. 1989. Outer membrane barrier as a mechanism of antimicrobial resistance. Antimicrob. Agents Chemother. 33:1831-1836.
41. Parkinson, J. S., and E. C. Kofoid. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26:71-112.
42. Pooley, H. M., and D. Karamata. 1994. Teichoic acid synthesis in Bacillus subtilis: genetic organization and biological role, p. 187-198. In J.-M. Ghuysen and R. Hakenbeck (ed.), Bacterial cell wall. Elsevier Sciences BV, Amsterdam, The Netherlands.
43. Popham, D. L., and P. Setlow. 1995. Cloning, nucleotide sequence, and mutagenesis of the Bacillus subtilis ponA operon, which codes for penicillin-binding protein (PBP) 1 and a PBP-related factor. J. Bacteriol. 177:326-335.
44. Puyet, A., and M. Espinosa. 1993. Structure of the maltodextrin-uptake locus of Streptococcus pneumoniae. Correlation to the Escherichia coli maltose regulon. J. Mol. Biol. 230:800-811.
45. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
46. Schonbachler, M., A. Horvath, J. Fassler, and H. Riezman. 1995. The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis. EMBO J. 14:1637-1645.
47. Sibold, C., J. Henrichsen, A. König, C. Martin, L. Chalkley, and R. Hakenbeck. 1994. Mosaic pbpX genes of major clones of penicillin-resistant Streptococcus pneumoniae have evolved from pbpX genes of a penicillin-sensitive Streptococcus oralis. Mol. Microbiol. 12:1013-1023.
48. Spratt, B. G. 1994. Resistance to β-lactam antibiotics, p. 517-534. In J.-M. Ghuysen and R. Hakenbeck (ed.), Bacterial cell wall. Elsevier Sciences BV, Amsterdam, The Netherlands.
49. Tiraby, J.-G., and M. S. Fox. 1974. Marker discrimination and mutagen-induced alterations in pneumococcal transformation. Genetics 77:449-458.
50. Tomasz, A., and J. L. Mosser. 1966. On the nature of the pneumococcal activator substance. Proc. Natl. Acad. Sci. USA 55:58-66.
51. Waks, S., and A. Tomasz. 1978. Secretion of cell wall polymers into the growth medium of lysis-defective pneumococci during treatment with penicillin and other inhibitors of cell wall synthesis. Antimicrob. Agents Chemother. 13:293-301.
52. Williamson, R., R. Hakenbeck, and A. Tomasz. 1980. In vivo interaction of β-lactam antibiotics with the penicillin-binding proteins of Streptococcus pneumoniae. Antimicrob. Agents Chemother. 18:629-637.
53. Zähner, D., T. Grebe, E. Guenzi, J. Krauß, M. van der Linden, K. Terhune, J. B. Stock, and R. Hakenbeck. 1996. Resistance determinants for β-lactam antibiotics in laboratory mutants of Streptococcus pneumoniae that are involved in genetic competence. Microb. Drug Res. 2:187-191.