High-stringency subtraction for the identification of differentially regulated cDNA clones

BioTechniques

Volumn 23, Issue 3, 1997, Pages 468-474

  Scutt, Charles P ^a   Gilmartin, Philip M ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; POLYADENYLATED RNA;

ARTICLE; CLONING VECTOR; DNA HYBRIDIZATION; DNA LIBRARY; IMAGE SUBTRACTION; NONHUMAN; PLANT; POLYMERASE CHAIN REACTION;

EID: 0030924012     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97233st04     Document Type: Article

References (9)

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2. Feinberg, A. and B. Vogelstein. 1983. A technique for labelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:6-13.
3. Houge, G. 1993. Simplified construction of a subtracted cDNA library using asymmetric PCR. PCR Methods Appl. 2:204-209.
4. Mason, P.J. and J.G. Williams. 1985. Hybridization in the analysis of recombinant DNA. p. 113-137. In B.D. Hames and S.J. Higgins (Eds.), Nucleic Acid Hybridization: A Practical Approach. IRL Press, Oxford.
5. Newton, C.R. and A. Graham. 1994. PCR. BIOS Scientific Publishers, Oxford.
6. Owens, G.P., W.E. Hahn and J.J. Cohen. 1991. Identification of mRNAs associated with programmed cell death in immature thymocytes. Mol. Cell. Biol. 11:4177-4188.
7. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
8. Swaroop, A., J. Xu, N. Agarwal and S.M. Weissman. 1991. A simple and efficient cDNA library subtraction procedure: isolation of human retina-specific cDNA clones. Nucleic Acids Res. 19:1954.
9. Young, B.D. and M.L.M. Anderson. 1985. Quantitative analysis of solution hybridization, p. 47-71. In B.D. Hames and S.J. Higgins (Eds.), Nucleic Acid Hybridization: A Practical Approach. IRL Press, Oxford.