Shiga toxin-producing Escherichia coli isolates from cases of human disease show enhanced adherence to intestinal epithelial (Henle 407) cells

Infection and Immunity

Volumn 65, Issue 9, 1997, Pages 3799-3805

  Paton, Adrienne W ^a   Voss, Elena ^a,b   Manning, Paul A ^b   Paton, James C ^a  

^a WOMEN S AND CHILDREN S HOSPITAL   (Australia)

^b UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

MANNOSE; SHIGA TOXIN; VIRULENCE FACTOR;

ARTICLE; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; BACTERIUM ISOLATION; CONTROLLED STUDY; DIARRHEA; EPIDEMIC; ESCHERICHIA COLI; FERMENTATION; FOOD CONTAMINATION; HEMOLYTIC UREMIC SYNDROME; HEMORRHAGIC COLITIS; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM CELL; MEAT; PRIORITY JOURNAL; SEROTYPE;

EID: 0030924006     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.65.9.3799-3805.1997     Document Type: Article

References (27)

1. Barrett, T. J., J. B. Kaper, A. E. Jerse, and I. K. Wachsmuth. 1992. Virulence factors in Shiga-like toxin-producing Escherichia coli isolated from humans and cattle. J. Infect. Dis. 165:979-980.
2. Beutin, L., D. Geier, S. Zimmermann, and H. Karch. 1995. Virulence markers of Shiga-like toxin-producing Escherichia coli strains originating from healthy domestic animals of different species. J. Clin. Microbiol. 33:631-635.
3. Blomfield, I. C., M. S. McClain, and B. I. Eisenstein. 1991. Type 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutants. Mol. Microbiol. 5: 1439-1445.
4. de Azavedo, J., E. McWhirter, M. Louie, and J. Brunton. 1994. EAE-negative verotoxin-producing Escherichia coli associated with hemolytic uremic syndrome and hemorrhagic colitis, p. 265-268. In M. A. Karmali and A. G. Goglio (ed.), Recent advances in verocytotoxin-producing Escherichia coli infections. Elsevier Science B.V., Amsterdam, The Netherlands.
5. Dytoc, M. T., A. Ismaili, D. J. Philpott, R. Soni, J. L. Brunton, and P. M. Sherman. 1994. Distinct binding properties of eaeA-negative Verocytotoxin-producing Escherichia coli of serotype O113:H21. Infect. Immun. 62:3494-3505.
6. Griffin, P. M. 1995. Escherichia coli O157:H7 and other enterohemorrhagic Escherichia coli, p. 739-761. In M. J. Blaser, P. D. Smith, J. I. Ravdin, H. B. Greenberg, and R. I. Guerrant (ed.), Infections of the gastrointestinal tract. Raven Press, New York, N.Y.
7. Karch, H., J. Heesemann, R. Laufs, A. D. O'Brien, C. O. Tacket, and M. M. Levine. 1487. A plasmid of enterohemorrhagic Escherichia coli O157:H7 is required for expression of a new fimbrial antigen and for adhesion to epithelial cells. Infect. Immun. 55:455-461.
8. Karmali, M. A. 1989. Infection by verotoxin-producing Escherichia coli. Clin. Microbiol. Rev. 2:15-38.
9. Kleanthous, H., H. R. Smith, S. M. Scotland, R. J. Gross, B. Rowe, C. M. Taylor, and D. V. Milford. 1990. Haemolytic uraemic syndromes in the British Isles, 1985-8: association with Verocytotoxin producing Escherichia coli, part 2. Microbiological aspects. Arch. Dis. Child. 65:722-727.
10. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
11. Louise, C. B., and T. G. Obrig. 1995. Specific interaction of Escherichia coli O157:H7-derived Shiga-like toxin II with renal endothelial cells. J. Infect. Dis. 172:1397-1401.
12. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
13. Manning, P. A. 1995. Use of confocal microscopy in studying bacterial adhesion and invasion. Methods Enzymol. 253:159-166.
14. McKee, M. L., and A. D. O'Brien. 1996. Truncated enterohemorrhagic Escherichia coli (EHEC) O157:H7 intimin (EaeA) fusion proteins promote adherence of EHEC strains to Hep-2 cells. Infect. Immun. 64:2225-2233.
15. Nishikawa, Y., S. M. Scotland, H. R. Smith, G. A. Willshaw, and B. Rowe. 1995. Catabolite repression of the adhesion of Vero cytotoxin-producing Escherichia coli of serogroups O157 and O111. Microb. Pathog. 18:223-229.
16. O'Brien, A. D., and R. K. Holmes. 1987. Shiga and shiga-like toxins. Microbiol. Rev. 51:206-220.
17. Ostroff, S. M., P. I. Tarr, M. A. Neill, J. H. Lewis, N. Hargrett-Bean, and J. M. Kobayashi. 1989. Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections. J. Infect. Dis. 160:994-999.
18. Paton, A. W., A. J. Bourne, P. A. Manning, and J. C. Paton. 1995. Comparative toxicity and virulence of Escherichia coli clones expressing variant and chimeric Shiga-like toxin type II operons. Infect. Immun. 63:2450-2458.
19. Paton, A. W., P. A. Manning, and J. C. Paton. 1995. Increased oral virulence of Escherichia coli expressing a variant Shiga-like toxin type II operon is associated with both A subunit residues Met4 and Gly102. Microb. Pathog. 19:185-191.
20. Paton, A. W., J. C. Paton, P. N. Goldwater, and P. A. Manning. 1993. Direct detection of Escherichia coli shiga-like toxin genes in primary fecal cultures using the polymerase chain reaction. J. Clin. Microbiol. 31:3063-3067.
21. Paton, A. W., R. Ratcliff, R. M. Doyle, J. Seymour-Murray, D. Davos, J. A. Lanser, and J. C. Paton. 1996. Molecular microbiological investigation of an outbreak of hemolytic-uremic syndrome caused by dry fermented sausage contaminated with Shiga-like toxin-producing Escherichia coli. J. Clin. Microbiol. 34:1622-1627.
22. Paton, J. C., and A. W. Paton. 1996. Survival rate of mice after transient colonization with Escherichia coli clones carrying variant Shiga-like toxin type II operons. Microb. Pathog. 20:377-383.
23. Schmidt, H., L. Beutin, and H. Karch. 1995. Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL933. Infect. Immun. 63:1055-1061.
24. Sherman, P. M., and R. Soni. 1988. Adherence of Vero cytotoxin-producing Escherichia coli of serotype O157:H7 to human epithelial cells in tissue culture: role of outer membranes as bacterial adhesins. J. Med. Microbiol. 26:11-17.
25. Tarr, P. I., S. S. Bilge, J. A. Vary, N. M. Tang, M. R. Baylor, K. Potter, T. E. Besser, and S. L. Moseley. 1995. Adherence and colonization mechanisms of Escherichia coli O157:H7, p. 119-124. In Abstracts of the 31st U.S.-Japan Cholera and Related Diarrheal Diseases Conference. U.S.-Japan Cooperative Medical Science Program, National Institutes of Health, Bethesda, Md.
26. Wadolkowski, E. A., L. M. Sung, J. A. Burris, J. E. Samuel, and A. D. O'Brien. 1990. Acute renal tubular necrosis and death of mice orally infected with Escherichia coli strains that produce Shiga-like toxin type II. Infect. Immun. 58:3959-3965.
27. Willshaw, G. A., S. M. Scotland, H. R. Smith, T. Cheasty, A. Thomas, and B. Rowe. 1994. Hybridization of strains of Escherichia coli O157 with probes derived from the eaeA gene of enteropathogenic E. coli and the eaeA homolog from a Vero cytotoxin-producing strain of E. coli O157. J. Clin. Microbiol. 32:897-902.