Delta 6-desaturase and delta-5-desaturase in human Hep G2 cells are both fatty acid interconversion rate limiting and are upregulated under essential fatty acid deficient conditions

Prostaglandins Leukotrienes and Essential Fatty Acids

Volumn 56, Issue 6, 1997, Pages 437-442

  Melin, T ^a   Nilsson, A ^a  

^a LUND UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ARACHIDONIC ACID; ICOSANOID; LINOLEIC ACID; LINOLENIC ACID;

ARTICLE; CELL STRAIN HEPG2; ENZYME ACTIVITY; ESSENTIAL FATTY ACID DEFICIENCY; FATTY ACID DESATURATION; FATTY ACID METABOLISM; HUMAN; HUMAN CELL; LIPID COMPOSITION; PRIORITY JOURNAL;

EID: 0030923290     PISSN: 09523278     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0952-3278(97)90596-2     Document Type: Article

References (25)

1. Holman R. T. Nutritional and metabolic interrelationships between fatty acids. Federatioin Proc 1964; 23: 1062-1067.
2. Sardesai V. M. Biochemical and nutritional aspects of eicosanoids. J Nutr Biochem 1992; 3: 562-579.
3. Chen Qi, Nilsson Å. Desaturation and chain elongatin of n-3 and n-6 polyunsaturated fatty acids in the human CaCo-2 cell line. Biochim Biophys Acta 1993; 1166: 193-201.
4. Furth E. E., Sprecher H., Fisher E. A., Fleishman H. D., Laposata M. An in vitro model for essential fatty acid deficiency: Hep G2 cells permanently maintained in lipid free medium J Lipid Res 1992; 33: 1719-1726.
5. Angeletti C., de-Alaniz M.J., Fatty acid uptake and metabolism in Hep G2 human hetoma cells. Mol Cell Biochem 1995; 143: 99-105.
6. Horrobin D. F. Nutritional and medical importance of gammalinolenic acid. Prog Lip Res 1992; 2: 163-194.
7. Grammatikos S. I., Subbaiah P. V., Victor T. A., Miller W. M. Diversity in the ability of cultured cells to elongate and desaturate essential n-6 and n-3 fatty acids. Ann N Y Acad Sci 1994; 745: 92-105.
8. Fujiyama-Fujiwara Y., Umeda R., Igarashi O. Metabolism of arachidonic eicosapentanoic, and docosahexaenoic acids in HepG2 cells and rat hepatocytes. J Nutr Sci Vitaminol Tokyo 1992; 38: 329-334.
9. Hrelia S., Bordoni A., Biagi P. L., Galeotti T., Palombini G., Masotti L. The role of delta-6 and delta-9-desaturase in the fatty acid metabolism of hepatomas with different growth rate. Biochem Mol Biol Int 1994; 34: 449-495.
10. Marzo I., Martinez-Lorenzo M. J., Anel A., et al. Biosynthesis of unsaturated fatty acids in the main cell linages of human leucemia and lymphoma. Biochim Biophys Acta 1995; 1257: 140-148.
11. de-Antueno R.J., Elliot M., Horrobin D. F. Liver delta-5 and delta-6 desaturase acitivity differs among laboratory rat strains. Lipids 1994; 29: 327-331.
12. Raederstorff D., Loechleiter V., Moser U. Polyunsaturated fatty acid metabolism of human fibroblasts during cellular ageing. Int J Vitam Nutr Res 1995; 65: 51-55.
13. Dinh T. K., Bure J. M., Durand G. 1993. Effect of age and alfalinoleic acid deficiency on delta-6-desaturase activity and liver lipids in rats. Lipids 1993; 28: 517-523.
14. Maniongui C., Blond J. P., Ulman L., Durand G., Poisson J-P., Bezard J. Age related changes in delta-6 and delta-5 desaturase activities in rat liver microsomes. Lipids 1993; 28: 291-297.
15. Biagi P. L., Bordoni A., Hrelia S., Celadon M., Horrobin D. F. Gamma-linolenic acid dietary supplemetation can reverse the aging influence on rat liver microsome delta-6-desaturase activity. Biochim Biophys Acta 1991; 1083: 187-192.
16. Vasques C. M., Muriana F.J., Ruiz-Gutierrez V. Changes in fatty acid desaturation in hepatic and intestinal tissues induced by intestinal resection. Lipids 1993; 28:471-473.
17. Horrobin D. F., Fatty acid metabolism in health and disease; the role of delta-6-desaturase. Am J Clin Nutr 1993; 57(suppl): 732S-737S.
18. Blond J. P. Bezard J. Delta-5-desaturation of dihomogammalinoleic acid into arachidonic acid by rat liver microsomes and incorporation of fatty acids in microsome phospholipids. Biochem Biophys Acta 1991; 1084: 255-260.
19. Evans J. F., Nathaniel D. J., Zamboni R. J., Ford-Hutchinson A. W. Leukotriene A3. A poor substrate but a potent inhibitor of rat and human neutrophil leukotriene A4 hydrolase. J Biol Chem 1985; 260: 10966-10970.
20. Hyman B. T., Stoll L. L, Spector A. A. Accumulation of n9-eicosatrienoic acid in confluent 3T3-L1 and 3T3 cells. J Biol Chem 1981; 256: 8863-8866.
21. Lerner R., Lindström P., Berg A., Johansson E., Rosendal K., Palmblad J. Development and characterizatoin of essential fatty acid deficiency in human endothelial cells in culture. Proc Natl Acad Sci USA 1995; 92: 1147-1151.
22. Laposata M., Prescott S. M., Bross T. E., Majerus P. W. Development and characterization of a tissue cuture cell line with essential fatty acid deficiency. Proc Natl Acad Sci USA 1982; 79: 7654-7658.
23. Marcelo C. L., Duell E. A., Rhodes L. M., Dunham W. R. In vitro model of essential fatty acid deficiency. J Invest Dermatol 1992; 99: 703-708.
24. Hjelte L., Melin T., Nilsson Å., Strandvik B. Absorbtion and metabolism of arachidonic and linoleic acid in essential fatty acid deficient rats. Am J Physiol 1991; 259: G1 16-G124.
25. Retterstol K., Woldseth B., Christoffersen B. O. Studies on the metabolism of mead acid in rat hepatocytes. Biochim Biophys Acta 1995; 1259: 82-88.