Saccharomyces cerevisiae Mpt5p interacts with Sst2p and plays roles in pheromone sensitivity and recovery from pheromone arrest

Molecular and Cellular Biology

Volumn 17, Issue 6, 1997, Pages 3429-3439

  Chen, Taosheng ^a,b   Kurjan, Janet ^a  

^a UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; MUTANT PROTEIN; PHEROMONE; PROTEIN SUBUNIT;

ARTICLE; CELL CYCLE G1 PHASE; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN INDUCTION; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; YEAST CELL;

SACCHAROMYCES CEREVISIAE;

EID: 0030923134     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.17.6.3429     Document Type: Article

References (72)

1. Akada, R., L., Kallal, D. I. Johnson, and J. Kurjan. 1996. Genetic relationships between the G protein βγ complex, Ste5p, Ste20p, and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics 143:103-117.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1989. Current protocols in molecular biology, p. 13.6.1-13.6.4. John Wiley & Sons, Inc., New York, N.Y.
3. Barker, D. D., C. Wang, J. Moore, L. K. Dickinson, and R. Lehman. 1992. Pumilio is essential for function but not for distribution of the Drosophila abdominal determinant Nanos. Genes Dev. 6:2312-2326.
4. Bartel, P., C.-T. Chien, R. Sternglanz, and S. Fields. 1993. Elimination of false positives that arise in using the two-hybrid system. BioTechniques 14:920-924.
5. Breeden, L., and K. Nasmyth. 1985. Regulation of the yeast HO gene. Cold Spring Harbor Symp. Quant. Biol. 50:643-650.
6. Brill, J. A., E. A. Elion, and G. R. Fink. 1994. A role for autophosphorylation revealed by activated alleles of FUS3, the yeast MAP kinase homolog. Mol. Biol. Cell 5:297-312.
7. Chan, R. K., and C. A. Otte. 1982. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and α factor pheromones. Mol. Cell. Biol. 2:11-20.
8. Chan, R. K., and C. A. Otte. 1982. Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and α factor pheromones. Mol. Cell. Biol. 2:21-29.
9. Chen, W., E. Balzi, E. Capieaux, and A. Goffeau. 1991. The YGL023 gene encodes a putative regulatory protein. Yeast 7:309-312.
10. Chien, C.-T., P. L. Bartel, R. Sternglanz, and S. Fields. 1991. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. USA 88:9578-9582.
11. Choi, K.-Y., B. Satterberg, D. M. Lyons, and E. A. Elion. 1994. Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell 78:499-512.
12. Christianson, T. W., R. S. Sikorski, M. Dante, J. H. Shero, and P. Hieter. 1992. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122.
13. Clare, J., and P. Farabaugh. 1985. Nucleotide sequence of a yeast Ty element: evidence for an unusual mechanism of gene expression. Proc. Natl. Acad Sci. USA 82:2829-2833.
14. mpk, the meiosis-activated myelin basic protein kinase. J. Biol. Chem. 266:15180-15184.
15. Coglievina, M., I. Bertani, R. Klima, P. Zaccaria, and C. V. Bruschi. 1995. The DNA sequence of a 7941 bp fragment of the left arm of chromosome VII of Saccharomyces cerevisiae contains four open reading frames including the multicopy suppressor of the pop2 mutation and a putative serine/threonine protein kinase gene. Yeast 11:767-774.
16. Courchesne, W. E., R. Kunisawa, and J. Thorner. 1989. A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae. Cell 58:1107-1119.
17. Cross, F. 1988. DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae. Mol. Cell. Biol. 8:4675-4684.
18. Cross, F. R. 1990. Cell cycle arrest caused by CLN gene deficiency in Saccharomyces cerevisiae resembles START-I arrest and is independent of the mating-pheromone signalling pathway. Mol. Cell. Biol. 10:6482-6490.
19. i3, is a member of a protein family with a highly conserved core domain. Proc. Natl. Acad. Sci. USA 92:11916-11920.
20. Dietzel, C., and J. Kurjan. 1987. The yeast SCG1 gene: a Gα-like protein implicated in the a- and α-factor response pathway. Cell 50:1001-1010.
21. Dietzel, C., and J. Kurjan. 1987. Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone. Mol. Cell. Biol. 7:4169-4177.
22. Dohlman, H. G., D. Apaniesk, Y. Chen, J. Song, and D. Nusskern. 1995. Inhibition of G-protein signaling by dominant gain-of-function mutations in Sst2p, a pheromone desensitization factor in Saccharomyces cerevisiae. Mol. Cell. Biol. 15:3635-3643.
23. Dohlman, H. G., J. Song, D. Ma, W. E. Courchesne, and J. Thorner. 1996 Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein α subunit). Mol. Cell. Biol. 16:5194-5209.
24. Dohlman, H. G., and J. Thorner. 1997. RGS proteins and signaling by heterotrimeric G proteins. J. Biol. Chem. 272:3871-3874.
25. Doi, A., and K. Doi. 1990. Cloning and nucleotide sequence of the CDC23 gene of Saccharomyces cerevisiae. Gene 91:123-126.
26. Dolan, J. W., C. Kirkman, and S. Fields. 1989. The yeast STE12 protein binds to the DNA sequence mediating pheromone induction. Proc. Natl. Acad. Sci. USA 84:5703-5707.
27. Ecker D. J., M. I. Khan, J. Marsh, T. R. Butt, and S. T. Crooke. 1987. Chemical synthesis and expression of a cassette adapted ubiquitin gene. J. Biol. Chem. 262:3524-3527.
28. Elion, E. A., J. A. Brill, and G. R. Fink. 1991. FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction. Proc. Natl. Acad. Sci. USA 88:9392-9396.
29. Elion, E. A., P. L. Grisafi, and G. R. Fink. 1990. FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation Cell 60:649-664.
30. Elion E. A., B. Satterberg, and J. E. Kranz. 1993. FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol. Biol. Cell 4:495-510.
31. Errede, B., and G. Ammerer. 1989. STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes. Genes Dev. 3:1349-1361.
32. Errede, B., A. Gartner, Z. Zhou, K. Nasmyth, and G. Ammerer. 1993. MAP kinase-related FUS3 from S. cerevisiae is activated by STE7 in vitro. Nature 362:261-264.
33. Hardy, C. F., L. Sussel, and D. Shore. 1992. A RAP1-interactmg protein involved in transcriptional silencing and telomere length regulation. Genes Dev. 6:801-814.
34. Harper, J. W., G. R. Adami, N. Wei, K. Keyomarsi, and S. J. Elledge. 1993. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75:805-816.
35. qα. Proc. Natl. Acad. Sci. USA 94:428-432.
36. Herskowitz, I., and R. E. Jensen. 1994. Putting the HO gene to work: practical uses for maling-type switching. Methods Enzymol. 194:132-146.
37. Iyengar, R. 1997. There are GAPS and there are GAPS. Science 275:42-43.
38. Kang, Y.-S., J. Kane, J. Kurjan, J. M. Stadel, and D. J. Tipper. 1990. Effects of expression of mammalian Gα and hybrid mammalian-yeast Gα proteins on the yeast pheromone response signal transduction pathway. Mol. Cell. Biol. 10:2582-2590.
39. Kikuchi, Y., Y. Oka, M. Kobayashi, Y. Uesono, A. Toh-E, and A. Kikuchi. 1994. A new yeast gene, HTR1, required for growth at high temperature, is needed for recovery from mating pheromone-induced G1 arrest. Mol. Gen. Genet. 245:107-116.
40. Kranz, J. E., B. Satterberg, and E. A. Elion. 1994. The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5. Genes Dev. 8:313-327.
41. Kurjan, J. 1993. The pheromone response pathway in Saccharomyces cerevisiae. Annu. Rev. Genet. 27:147-179.
42. Leberer, E., D. Dignard, D. Harcus, D. Y. Thomas, and M. Whiteway. 1992. The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein βγ subunits to downstream signalling components. EMBO J. 11:4815-4824.
43. Lee, B. N., and T. H. Adams. 1994. Overexpression of flbA, an early regulator of Aspergillus asexual sporulation, leads to activation of brlA and premature initiation of development. Mol. Microbiol. 14:323-324.
44. Levin, D. E., and B. Errede. 1995. The proliferation of MAP kinase signaling pathways in yeast. Curr. Opin. Cell Biol. 7:197-202.
45. 1 cyclins differ in their intrinsic functional specificities. Mol. Cell. Biol. 16: 6794-6803.
46. Macdonald, P. M. 1992. The Drosophila pumilio gene: an unusually long transcription unit and an unusual protein. Development 114:221-232.
47. Marcus, S., A. Polverino, M. Barr, and M. Wigler. 1994. Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module. Proc. Natl. Acad. Sci. USA 91:7762-7766.
48. Marsh, L., A. M. Neiman, and I. Herskowitz. 1991. Signal transduction during pheromone response in yeast. Annu. Rev. Cell Biol. 7:699-728.
49. Mitchell, D. A., T. K. Marshall, and R. J. Deschenes. 1993. Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeast. Yeast 9:715-723.
50. Miyajima, I., M. Nakafuku, N. Nakayama, C. Brenner, A. Miyajima, K. Kaibuchi, K. Arai, Y. Kaziro, and K. Matsumoto. 1987. GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction. Cell 50:1011-1019.
51. Moore, S. A. 1983. Comparison of dose-responsive curves for alpha factor-induced agglutinin, cell division arrest, and projection formation of Saccharomyces cerevisiae MATa yeast cells. J. Biol. Chem. 258:13849-13856
52. Murata, Y., and R. P. Wharton. 1995. Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos. Cell 80:747-756.
53. Özcan S., K. Freidel, A. Leuker, and M. Ciriacy. 1993. Glucose uptake and catabolite repression in dominant HTR1 mutants of Saccharomyces cerevisiae. J. Bacteriol. 175:5520-5528.
54. Pelech S. L., and J. S. Sanghera. 1992. Mitogen-activated protein kinases: versatile transducers for cell signaling. Trends Biochem. Sci. 17:233-238.
55. Peter, M., A. Gartner, J. Horecka, G. Ammerer, and I. Herskowitz. 1993. FAR1 links the signal transduction pathway to the cell cycle machinery in yeast. Cell 73:747-760.
56. Peter, M., and I. Herskowitz. 1994. Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1. Science 265:1228-1231.
57. Printen, J. A., and G. F. Sprague, Jr. 1994. Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics 138:609-619.
58. Ramer S. W., and R. W. Davis. 1993. A dominant truncation allele identities a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 90:452-456.
59. Richardson, H. E., C. Wittenberg, F. Cross, and S. I. Reed. 1989. An essential G1 function for cyclin-like proteins in yeast. Cell 59:1127-1133.
60. Rothstein, R. J. 1983. One-step gene disruption in yeast. Methods Enzymol. 101:202-211.
61. Sakai, A., T. Chibazakura, Y. Shimizu, and F. Hishinuma. 1992. Molecular analysis of POP2 gene, a gene required for glucose-derepression of gene expression in Saccharomyces cerevisiae. Nucleic Acids Res. 20:6227-6233.
62. Schreuder, M. P., S. Brekelmans, H. van den Ende, and F. M. Klis. 1993. Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae. Yeast 9:399-409.
63. Seifert, H. S., E. Y. Chen, M. So, and F. Heffron. 1986. Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 83:737-739.
64. Sikorski, R. S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27.
65. Song, O.-K., J. W. Dolan, Y.-L. O. Yuan, and S. Fields. 1991. Pheromone-dependent phosphorylalion of the yeast STE12 protein correlates with transcriptional activation. Genes Dev. 5:741-750.
66. Trueheart, J., J. D. Boeke, and G. R. Fink. 1987. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein. Mol. Cell. Biol. 7:2316-2328.
67. 1-phase Cdc28 kinase complexes. Mol. Cell. Biol. 13:5659-5669.
68. Tyers, M., G. Tokiwa, and B. Futcher. 1993. Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2, and other cyclins. EMBO J. 12:1955-1968.
69. Tyers, M., G. Tokiwa, R. Nash, and B. Futcher. 1992. The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation. EMBO J. 11:1773-1784.
70. Whiteway, M., L. Hougan, D. Dignard, D. Y. Thomas, L. Bell, G. C. Saari, F. J. Grant, P. O'Hara, and V. L. MacKay. 1989. The STE4 and STE18 genes of yeast encode potential β and γ subunits of the mating factor receptor-coupled G protein. Cell 56:467-477.
71. Yu, J.-H., J. Wieser, and T. H. Adams. 1996. The Aspergillus FlbA RGS domain protein antagonizes G protein signaling to block proliferation and allow development. EMBO J. 15:5184-5190.
72. Zoller, M. J., and M. Smith. 1984. Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template. DNA 3:479-488.