Inhibition of serine-threonine protein phosphatases decreases barrier function of rat pulmonary microvascular endothelial cells

Journal of Cellular Physiology

Volumn 171, Issue 3, 1997, Pages 259-270

  Diwan, Abdul H ^a   Honkanen, Richard E ^b   Schaeffer Jr , Richard C ^c   Strada, Samuel J ^a   Thompson, William J ^a  

^a UNIVERSITY OF SOUTH ALABAMA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF SOUTH ALABAMA   (United States)

^c VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ATRIAL NATRIURETIC FACTOR; CALYCULIN A; CYCLIC GMP; ENZYME INHIBITOR; KT 5926; MYOSIN LIGHT CHAIN KINASE; OKADAIC ACID; PHOSPHODIESTERASE INHIBITOR; PHOSPHOPROTEIN PHOSPHATASE; ROLIPRAM;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL MEMBRANE PERMEABILITY; ENDOTHELIUM CELL; ENZYME INHIBITION; LUNG BLOOD VESSEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RAT; VASCULAR ENDOTHELIUM;

ANIMALS; CYCLIC GMP; ENDOTHELIUM, VASCULAR; ENZYME INHIBITORS; INTERCELLULAR JUNCTIONS; LUNG; MICROCIRCULATION; OKADAIC ACID; OXAZOLES; PHOSPHOPROTEIN PHOSPHATASE; RATS;

EID: 0030922645     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4652(199706)171:3<259::AID-JCP4>3.0.CO;2-N     Document Type: Article

References (35)

1. Adkins, W.K., and Taylor, A.E. (1990) Role of xanthine oxidase and neutrophils in ischemia-reperfusion injury in rabbit lung. J. Appl. Physiol., 69:2012-2018.
2. Albelda, S.M., Sampson, P.M., Haselton, F.R., McNiff, J.M., Mueller, S.N., Williams, S.K., Fishman, A.P., and Levine, E.M. (1988) Permeability characteristics of cultured endothelial cell monolayers. J. Appl. Physiol., 64:308-322.
3. Ämmälä, C., Eliasson, L., Bokvist, K., Berggren, P.-O., Honkanen, R.E., Sjöholm, Å., and Rorsman P. (1995) Activation of protein kinases and inhibition of protein phosphatases play a central role in the regulation of exocytosis in mouse pancreatic β-cells. Proc. Natl. Acad. Sci. U. S. A., 91:4343-4347.
4. Barnard, J.W., Seibert, A.F., Prasad, R., Smart, D., Strada, S.J., Taylor, A.E., and Thompson, W.J. (1994) Reversal of pulmonary capillary ischemia-reperfusion injury by rolipram, a cAMP phosphodiesterase inhibitor. J. Appl. Physiol., 77:774-781.
5. Cooper, M.A., Del Vecchio, P.J., Minnear, F.L., Burhop, K.E., Selig, W.M., Garcia, J.G.N., and Malik, A.B. (1987) Measurement of albumin permeability across endothelial monolayers in vitro. J. Appl. Physiol., 62:1076-1083.
6. Critz, S.D., and Honkanen, R.E. (1995) Protein phosphatases and their role in the regulation of membrane currents in sensory neurons of Aplysia. Neuroprotocols, 6:78-83.
7. deLanerolle, P., Nishikawa, M., Yost, D.A., and Adelstein, R.S. (1984) Increased phosphorylation of myosin light chain kinase after an increase in cyclic AMP in intact smooth muscle. Science, 223:1415-1417.
8. Diwan, A.H., Thompson, W.J., Lee, A.K., and Strada, S.J. (1994) Cyclic GMP-dependent protein kinase activity in rat pulmonary microvascular endothelial cells. Bicohem. Biophys. Res. Commun., 202:728-735.
9. Eaton, R.M., Toothill, V.J., Davies, A., Pearson, J.D., and Mann, G.E. (1991) Permeability of human venous endothelial cell monolayers perfused in microcarrier cultures: Effects of flow rate, thrombin and cytochalasin D. J. Cell. Phsyiol., 149:88-99.
10. Farrukh, I.S., Gurtner, G.H., and Michael, J.R. (1987) Pharmacological modification of pulmonary vascular injury: Possible role of cAMP. J. Appl. Physiol., 62:47-54.
11. Ferro, T.J., Lynch, J.S., and Malik, A.B. (1989) Macrophages activated by fibrin increase albumin permeability across pulmonary artery endothelial monolayers. Am. Rev. Resp. Dis., 139:940-945.
12. Garcia, J.G., and Schaphorst, K.L. (1995) Regulation of endothelial cell gap formation and paracellular permeability. J. Invest. Med., 43:117-126.
13. Gibson, S., Goeckeler, Z.M., and Wysolmerski, R.B. (1996) Functional implications of cAMP on endothelial cell isometric tension. FASEB J., 10:3589.
14. Goeckeler, Z.M., and Wysolmerski, R.B. (1995) Myosin light chain kinase-regulated endothelial cell contraction: Relationship between isometric tension, actin polymerization, and myosin phosphorylation J. Cell. Biol., 130:613-627.
15. Hathaway, D.R., Eaton, C.R., and Adelstein, R.S. (1981) Regulation of human platelet myosin light chain kinase by the catalytic subunit of cyclic AMP-dependent protein kinase. Nature, 291:252-254.
16. Haynes, J., Jr., Seibert, A.F., Bass, J.B., and Taylor, A.E (1990) U74500A inhibition of oxidant mediated lung injury. Am. J. Physiol., 259:H144-H148. 1990.
17. Hempel, A., Noll, T., Muhs, A., and Piper, H.M. (1996) Functional anatagonism between cAMP and cGMP on permeability of coronary endothelial monolayers. Am. J. Physiol., 270:H1264-H1271.
18. Honkanen, R.E., Dukelow, M., Zwiller, J., Moore, R.E., Khatra, B.S., and Boynton, A.L. (1991) Cyanobacterial nodularin is a potent inhibitor of type I and type 2A protein phosphatases. Mol. Pharmacol., 40:577-583.
19. Katz, M.A., and Schaeffer, R.C., Jr. (1991) Convection of macromolecules is the dominant mode of transport across horizontal 0.4 and 3 μm filters in diffusion chambers: Significance for biologic monolayer permeability assessment. Microvasc. Res., 41:149-163.
20. Katz, M.A., Thompson, W.J., Diwan, A.H., Strada, S.J., and Schaeffer, R.S. (submitted).
21. Koga, S., Morris, S., Ogawa, S., Liao, H., Bilezikian, J.P., Chen, G., Thompson, W.J., Ashikaga, T., Brett, J., and Stern, D.M. (1995) TNF modulates endothelial permeability properties by decreasing cAMP. Am. J. Physiol., 268:C1104-C1113.
22. ++ and inositol polyphosphates. Am. J. Physiol., 263:L219-225.
23. Martin, W., and Luck, J.F. (1991) Enhancement of arterial endothelial barrier function by β-adrenoceptor agonists. Br. J. Pharmacol., 102:13-18.
24. Minnear, F.L., De Michele, M.A., Moon, D.G., Rieder, C.L., and Fenton, J.W., III (1989) Isoproterenol reduces thrombin-induced pulmonary endothelial permeability in vitro. Am. J. Physiol., 257:H1613-H1623.
25. Noel, P.E., Fletcher, J.R., and Thompson, W.J. (1995) Rolipram and isoproterenol reverse platelet activating factor-induced increases in pulmonary microvascular permeability and vascular resistance. J. Surg. Res., 59:159-164.
26. Pereschini, A., Kamm, K.E., and Stull J.T. (1986) Different phosphorylated forms of myosin in contracting tracheal smooth muscle. J. Biol. Chem., 261:6293-6299.
27. Schaeffer, R.C., Jr., and Bitrick, M.S. (1995) Effects of human α-thrombin and 8bromo-cAMP on large and microvessel endothelial monolayer equivalent "pore" radii. Microvasc. Res., 49:364-371.
28. Schaeffer, R.C., Jr., Gong, F.C., Bitrick, M.S., Jr., and Smith, T.L. (1993) Thrombin and bradykinin initiate distinct endothelial solute permeability mechanisms. Am. J. Physiol., 33:H1798-H1809.
29. Schnitzer, J.E., Siflinger-Birnboim, A., Del Vecchio, P.J., and Malik, A.B. (1994) Segmental differentiation of permeability, protein glycosylation, and morphology of cultured bovine lung vascular endothelium. Biochem. Biophys, Res. Commun., 199:11-19.
30. Sekhar, K.R., Hatchett, R.J., Shabb, J.B., Wolfe, L., Francis, S.H., Wells, J.N., Jastorff, B., Butt, E., Chakinala, M.M., and Corbin, J.D. (1992) Relaxation of pig coronary arteries by new and potent cGMP analogs that selectively activate type Iα, compared with type Iβ, cGMP-dependent protein kinase. Mol. Pharmacol., 42:103-108.
31. Shasby, D.M., Kamath, J.M., Moy, A.B., and Shasby, S.S. (1995) Ionomycin and PDBU increase MDCK monolayer permeability independently of myosin light chain phosphorylation. Am. J. Physiol., 269:L144-L150.
32. Sheldon, R., Moy, A., Lindsley, K., Shasby, S., and Shasby, D.M. (1993) Role of myosin light chain phosphorylation in endothehal cell retraction Am. J. Physiol., 265:L606-L612.
33. Thompson, W.J., Danthuluri, S., and Strada, S.J. (1993) Rolipram (ROL)-sensitive cyclic nucleotide (CN) phosphodiesterase (PDE) isozyme regulates cAMP metabolism in pulmonary microvascular endothelial cells (PMVEC). FASEB J., 7:A359.
34. Venn, A.D., Patterson, C.E., Day, M.A., and Garcia, J.G. (1995) Regulation of endothelial cell gap formation and barrier function by myosin-associated phosphatase activities. Am. J. Physiol., 269:L99-L108.
35. Westendorp, R.G.J., Draijer, R., Meinders, A.E., and van Hinsbergh, V.W. (1993) Cyclic GMP-mediated decrease in permeability of human umbilical and pulmonary artery endothelial cell monolayers. J. Vasc. Res., 31:42-51.