Molecular characterization of the B-box protein-protein interaction motif of the ETS-domain transcription factor Elk-1

EMBO Journal

Volumn 16, Issue 9, 1997, Pages 2431-2440

  Ling, Yan ^a   Lakey, Jeremy H ^a   Roberts, Claire E ^a   Sharrocks, Andrew D ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

Elk 1; ETS domain; Protein protein interactions; Transcription factors

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE; TRANSCRIPTION FACTOR;

ALPHA HELIX; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; HYDROPHOBICITY; MOLECULAR MODEL; MOUSE; NONHUMAN; ONCOGENE C FOS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

3T3 CELLS; ALANINE; AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; BINDING SITES; CIRCULAR DICHROISM; DNA-BINDING PROTEINS; ETS-DOMAIN PROTEIN ELK-1; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEAR PROTEINS; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTO-ONCOGENE PROTEINS; RECEPTOR PROTEIN-TYROSINE KINASES; SERUM RESPONSE FACTOR; STRUCTURE-ACTIVITY RELATIONSHIP; TRANSCRIPTION FACTORS;

ANIMALIA; CERVUS ELAPHUS;

EID: 0030921505     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.9.2431     Document Type: Article

References (44)

1. Bordo, D. and Argos, P. (1991) Suggestions for 'Safe' residue substitutions in site-directed mutagenesis. J. Mol. Biol., 217, 721-729.
2. Boshart, M., Kluppel, A., Schmidt, A., Schütz, G. and Luckow, B. (1992) Reporter contructs with low background activity utilizing the CAT gene. Gene, 110, 129-130.
3. Cahill, M.A., Janknecht, R. and Nordheim, A. (1996) Signalling pathways: Jack of all cascades. Curr. Biol., 6, 16-19.
4. Carlsson, P., Waterman, M.L. and Jones, K.A. (1993) The hLEF/TCF1α HMG protein contains a context-dependent transcriptional activation domain that induces the TCRα enhancer in T cells. Genes Dev., 7, 2418-2430.
5. Chang, C.-P., Shen, W.-P., Rozenfeld, S., Lawrence, H.J., Largman, C. and Cleary, M.L. (1995) Pbx protein display hexapeptide-dependent cooperative DNA binding with a subset of Hox protein. Genes Dev., 9, 663-674.
6. Chen, Y.H., Yang, J.T. and Martinez, H.M. (1972) Determination of the secondary structures of globular proteins by circular dichroism and optical dispersion. Biochemistry, 11, 4120-1131.
7. Crepieux, P., Coll, J. and Stehelin, D. (1994) The Ets-family of proteins: weak modulators of gene expression in quest for transcriptional partners. Crit. Rev. Oncogen., 5, 615-638.
8. Cunningham, B.C. and Wells, J.A. (1989) High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science, 244, 1081-1085.
9. Dalton, S. and Treisman, R. (1992) Characterisation of SAP-1, a protein recruited by serum response factor to the c-fos serum response element. Cell, 68, 597-612.
10. Gill, S.C. and Von Hippel, P.H. (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem., 182, 319-326.
11. TCF by MAP kinase stimulates ternary complex formation at the c-fos promoter. Nature, 358, 415-417.
12. Gille, H., Kortenjann, M., Thomae, O., Moomaw, C., Slaughter, C., Cobb, M.H. and Shaw, P.E. (1995a) ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation. EMBO J., 14, 951-962.
13. Gille, H., Strahl, T. and Shaw, P.E. (1995b) Activation of ternary complex factor Elk-1 by stress-activated protein kinases. Curr. Biol., 5, 1191-1200.
14. Giovane, A., Pintzas, A., Maira, S-M., Sobieszcuk, P. and Wasylyk, B. (1994) Net, a new ets transcription factor that is activated by Ras. Genes Dev., 8, 1502-1513.
15. Hill, C.S., Marais, R., John, S., Wynne, J., Dalton, S. and Treisman, R. (1993) Functional analysis of a growth factor-responsive transcription factor complex. Cell, 73, 395-406.
16. Holloway, J.M., Szeto, D.P., Scully, K.M., Glass, C.K. and Rosenfeld, M.G. (1995) Pit-1 binding to specific DNA sites as a monomer or dimer determines gene-specific use of a tyrosine-dependent synergy domain. Genes Dev., 9, 1992-2006.
17. Janknecht, R. and Nordheim, A. (1992) Elk-1 protein domains required for direct and SRF-assisted DNA-binding. Nucleic Acids Res., 20. 3317-3324.
18. Janknecht, R., Zinck, R., Ernst, W.H. and Nordheim, A. (1994) Functional dissection of the transcription factor Elk-1. Oncogene, 9, 1273-1278.
19. Knoepfler, P.S. and Kamps, M.P. (1995) The peptapeptide motif of Hox proteins is required for co-operative DNA binding with Pbx-1, physically contacts Pbx-1 and enhances DNA binding by Pbx-1. Mol. Cell. Biol., 15, 5811-5819.
20. Lehrman, S.R., Tuls, J.L. and Lund, M. (1990) Peptide alpha-helicity in aqueous trifluoroethanol: correlations with predicted alpha-helicity and the secondary structure of the corresponding regions of bovine growth hormone. Biochemistry, 29, 5590-5596.
21. Li, T., Stark, M.R., Johnson, A.D. and Wolberger, C. (1995) Crystal structure of the MATa1/MATα2 homeodomain heterodimer bound to DNA. Science, 270, 262-269.
22. Lin, J., Chen, J., Elenbaas, B. and Levine, A.J. (1994) Several hydrophobic amino acids in the p53 amino-terminal domain are required for transcriptional activation, binding to mdm-2 and the adenovirus 5 E1B 55-kD protein. Genes Dev., 8, 1235-1246.
23. Lopez, M. Oettgen, P., Akbarali, Y., Dendorfer, U. and Liberman, T.A. (1994) ERP, a new member of the ets transcription factor/oncoprotein family: cloning, characterization, and differential expression during B-lymphocyte development. Mol. Cell. Biol., 14, 3292-3309.
24. Marais, R., Wynne, J. and Treisman, R. (1993) The SRF accessory protein Elk-1 contains a growth factor-regulated transcriptional activation domain. Cell, 73, 381-393.
25. Mead, J., Zhong, H., Acton, T.B. and Vershon, A.K. (1996) The yeast α2 and Mcm 1 proteins interact through a region similar to a motif found in homeodomain proteins of higher eukaryotes. Mol. Cell. Biol., 16, 2135-2143.
26. Mueller, C.G.F. and Nordheim, A. (1991) A protein domain conserved between yeast mcm1 and human SRF directs ternary complex formation. EMBO J., 10, 4219-4229.
27. Price, M.A., Rogers, A.E. and Treisman, R. (1995) Comparative analysis of the ternary complex factors Elk-1, SAP-1a and SAP-2 (ERP/NET). EMBO J., 14, 2589-2601.
28. Pu, W.T. and Struhl, K. (1993) Dimerisation of leucine zippers analyzed by random selection. Nucleic Acids Res., 21, 4348-4355.
29. Rao, V.N., Huebner, K., Isobe, M., Ab-Rushidi, A., Croce, C.M. and Reddy, E.S.P. (1989) Elk-1, tissue-specific Ets-related genes on chromosomes X and 14 near translocation breakpoints. Science, 244, 66-70.
30. Sharrocks, A.D. (1995) ERK2/p42 MAP kinase stimulates both autonomous and SRF-dependent DNA binding by Elk-1. FEBS Lett., 368, 77-80.
31. SRF: implications for a novel DNA-binding motif. Mol. Cell. Biol., 13, 123-132.
32. SRF. EMBO J., 11, 3011-3019.
33. Shore, P. and Sharrocks, A.D. (1994) The transcription factors Elk-1 and serum response factor interact by direct protein-protein contacts mediated by a short region of Elk-1. Mol. Cell. Biol. 14, 3283-3291.
34. Shore, P. and Sharrocks, A.D. (1995a) The MADS-box family of transcription factors. Eur. J. Biochem., 229, 1-13.
35. Shore, P. and Sharrocks, A.D. (1995b) The ETS-domain transcription factors Elk-1 and SAP-1 exhibit differential DNA binding specificities. Nucleic Acids Res., 23, 4698-4706.
36. Shore, P., Whitmarsh, A.J., Bhaskaran, R., Davis, R.J., Waltho, J.P. and Sharrocks, A.D. (1996) Determinants of DNA-binding specificity of ETS-domain transcription factors. Mol. Cell. Biol., 16, 3338-3349.
37. Stark, M.R. and Johnson, A.D. (1994) Interaction between two homedomain proteins is specified by a short C-terminal tail. Nature, 371, 429-432.
38. Treisman, R. (1985) Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5′ element and c-fos 3′ sequence. Cell, 42, 889-902.
39. Treisman, R. (1994) Ternary complex factors: growth regulated transcriptional activators. Curr. Opin. Genet. Dev., 4, 96-101.
40. Treisman, R., Marais, R. and Wynne, J. (1992) Spatial flexibility in ternary complexes between SRF and its accessory proteins. EMBO J., 11, 4631-4640.
41. Van Hoy, M., Leuther, K.K., Kodadek, T. and Johnston, S.A. (1993) The acidic activation domain of the GCN4 and GAL4 proteins are not alpha helical but form beta sheets. Cell, 72, 587-594.
42. Vinson, C.R., Hai, T. and Boyd, S.M. (1993) Dimerisation specificity of the leucine zipper containing bZIP motif on DNA binding: prediction and rational design. Genes Dev., 7, 1047-1058.
43. Whitmarsh, A.J., Shore, P., Sharrocks, A.D. and Davis, R.J. (1995) Integration of MAP kinase signal transduction pathways at the serum response element. Science, 269, 403-107.
44. Williams, K.A. and Deber, C.M. (1991) Proline residues in transmembrane helices structural or dynamic role? Biochemistry, 30, 8919-8923.