Keratin intermediate filament dynamics in cell heterokaryons reveals diverse behaviour of different keratins

Journal of Cell Science

Volumn 110, Issue 9, 1997, Pages 1099-1111

  Paramio, Jesús M ^a   Casanova, M Llanos ^a   Alonso, Angel ^b   Jorcano, José L ^a  

^a CIEMAT   (Spain)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Cell fusion; Cytoskeleton dynamics; Intermediate filament; Keratin

Indexed keywords

KERATIN;

ARTICLE; CELL FUSION; CROSS LINKING; CYTOSKELETON; DEPOLYMERIZATION; EPITHELIUM CELL; HETEROKARYON; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; INTERMEDIATE FILAMENT; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

ANIMALS; CATTLE; CELL LINE; CYTOSKELETON; FLUORESCENT ANTIBODY TECHNIQUE; HYBRID CELLS; KERATIN-10; KERATINS; MACROPODIDAE; MAMMARY GLANDS, ANIMAL; POLYETHYLENE GLYCOLS; SOLUBILITY;

EID: 0030920655     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Achtstatter, T., Hatzfeld, M., Quinlan, R. A., Parmelee, D. C. and Franke, W. W. (1986). Separation of cytokeratin polypeptides by gel elctrophoretic and chromatographic techniques and their identification by immunoblotting. Meth. Enzymol. 134, 355-371.
2. Albers, K. and Fuchs, E. (1987). The expression of mutant epidermal keratin cDNAs transfected in simple epithelial and squamous cell carcinoma lines J. Cell Biol. 105, 791-806.
3. Albers, K. and Fuchs, E. (1989). Expression of mutant keratin cDNAs in epithelial cells reveals possible mechanisms for initiation and assembly of intermediate filaments. J. Cell Biol. 108, 1477-1493.
4. Blessing, M., Jorcano, J. L. and Franke, W. W. (1989). Enhancer elements directing the cell type-specific expression of cytokeratin genes and changes of the epithelial cytoskeleton by transfections of hybrid cytokeratin genes. EMBO J. 8, 117-126.
5. Bloemendal, H. and Pieper, F. R. (1989). Intermediate filaments: known structure, unknown function. Biochim. Biophys. Acta 1007, 245-253.
6. Chin, S. S. M. and Liem, R. K. H. (1989). Expression of rat neurofilament proteins NF-L and NF-M in transfected non-neuronal cells. Eur. J. Cell Biol. 50, 475-490.
7. Chou, C. F. and Omary, M. B. (1991). Phorbol acetate enhances the phosphorylation of cytokeratin 8 and 18 in human colonic epithelial cells. FEES Lett. 282, 200-204.
8. Chou, C. F., Riopel, C. L., Roll, L. S. and Omary, M. B. ( 1993). A significant soluble keratin fraction in 'simple' epithelial cells. Lack of an apparent phosphorylation and glycosylation role in keratin solubility. J. Cell Sci. 105, 433-444.
9. Chou, V. H., Rosevear, E. and Goldman, R. D. (1989). Phosphorylation and disassembly of intermediate filaments in mitotic cells. Proc. Nat. Acad. Sci. USA 86, 1885-1889.
10. cdc2 phosphorylation of vimentin. Cell 62, 1063-1071.
11. Cooper, D., Schermer, A. and Sun, T. T. (1985). Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations. Lab. Invest. 52, 243-256.
12. Coulomhe, P. A. and Fuchs, E. (1990). Elucidating the early stages of keratin filament assembly. J. Cell Biol. 111, 153-169.
13. Eichner, R., Sun, T. T. and Aebi, U. (1986). The role of keratin subfamilies and keratin pairs in the formation of human epidermal intermediate filaments. J. Cell Biol. 102, 1767-1777.
14. Franke, W. W., Schiller, D. L. and Grund, C. (1982). Intermediate filament proteins in nonfilamentous structures: Transient disintegration and inclusion of subunit proteins in granular aggregates. Cell 30, 103-113.
15. Franke, W. W., Schiller, D. L., Halzfeld, M. and Winter, S. (1983). Protein complexes of intermediate-sized filaments: melting of cytokeratins complexes in urea reveals different polypeptide separation characteristics. Proc. Nat. Acad. Sci. USA 80, 7113-7117.
16. Franke, W. W., Schmid, E., Mittnacht, S., Grund, C. and Jorcano, J. L. (1984). Integration of different keratins into the same filament system after microinjection of mRNA for epidermal keratins into kidney epithelial cells. Cell 36, 813-825.
17. Fuchs, E., Coppock, S. M., Green, H. and Cleveland, D. W. (1981). Two distinct classes of keratin genes and their evolutionary significance. Cell 27, 75-84.
18. Fuchs, E. and Weber, K. (1994). Intermediate filaments: structure, dynamics, function and disease. Annu. Rev. Biochem. 63, 345-382.
19. Gawlitta, W., Stocke, W., Wehland, J. and Weber, K. (1980). Organization and spatial arrangement of fluorescein-labeled native actin microinjected into normal locomoting and experimentally influenced Amoeba proteus. Cell Tissue Res. 206, 181-191.
20. Glacy, S. L. (1983). Pattern and time course of rhodamine-actin incorporation into cardiac myocytes. J. Cell Biol. 96, 1162-1167.
21. Green, K. C. and Jones, J. C. R. (1990). Interaction of intermediate filaments with the cell surface. In Cellular and Molecular Biology of Intermediate Filaments (ed. R. D. Goldman and P. M. Steinert), pp. 147-174. New York: Plenum Publishing Corp.
22. Gruen, L. C. and Woods, E. F. (1983). Structural studies on the microfibrillar proteins of wool. Biochem. J. 209, 587-595.
23. Hatzfeld, M. and Franke, W. W. (1985). Pair formation and promiscuity of cytokeratins: formation in vitro of heterotypic complexes and intermediatesized filaments by homologous and heterologous recombinations of purified polypeptides. J. Cell Biol. 101, 1826-1841.
24. Hatzfeld, M. and Weber, K. (1990). The coiled-coil of in vitro assembled keratin filaments is a heterodimer of type I and type II keratins: use of site specific mutagenesis and recombinant protein expression. J. Cell Biol. 110, 1199-1210.
25. Huszar, M., Gigi-Leitner, O., Moll, R., Franke, W. W. and Geiger, B. (1986). Monoclonal antibodies to various acidic (type I) cytokeratins of stratified epithelia. Selective markers for stratification and squamous cell carcinomas. Differentiation 31, 141-153.