Gene cloning, sequencing and enzymatic properties of glutamate synthase from the hyperthermophilic archaeon Pyrococcus sp. KOD1

Molecular and General Genetics

Volumn 254, Issue 6, 1997, Pages 635-642

  Jongsareejit, B ^a   Rahman, R N Z A ^a   Fujiwara, S ^a   Imanaka, T ^b  

^a OSAKA UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

Gene expression; Glutamate synthase; Hyperthermophilic archaea; Nitrogen assimilation; Pyrococcus

Indexed keywords

BACTERIAL ENZYME; CYSTEINE; GLUTAMATE SYNTHASE; GLUTAMIC ACID;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENETICS; CONSENSUS SEQUENCE; ESCHERICHIA COLI; GENE SEQUENCE; MOLECULAR CLONING; NITROGEN FIXATION; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; ARCHAEA; BASE SEQUENCE; CHROMOSOMES, BACTERIAL; CLONING, MOLECULAR; EUKARYOTIC CELLS; GLUTAMATE SYNTHASE; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0030919815     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050461     Document Type: Article

References (35)

1. Anderson MP, Vance CP, Heichel GH, Miller SS (1989) Purification and characterization of NADH-glutamate synthase from alfalfa root nodules. Plant Physiol 90:351-358
2. Bravo A, Mora J (1988) Ammonium assimilation in Rhizobium phaseoli by the glutamine synthetase-alutamate synthase pathway. J Bacteriol 170:980-984
3. Bull CJ, White O, Olsen GJ, Zhou L, Fleishmann RD, Sutton GG, Blake JA, FitzGerald LM, Clayton RA, Gocayne JD, Kerlavage AR, Dougherty BA, Tomb J-F, Adams MD, Reich CI, Overbeek R, Krikness EF, Weinstock KG, Merrick JM, Glodek A, Scott JL, Geoghagen NSM, Weidman JF, Fuhrmann JL, Nguyen D, Utterback TR, Kelley JM, Peterson JD, Sadow PW, Hanna MC, Cotton MD, Roberts KM, Hurst MA, Kaine BP, Borodovsky M, Klenk H-P, Fraser CM, Smith HO, Woese CR, Venter JC (1996) Complete genome sequence of the methanogenic archaeon. Methanococcus jannaschii. Science 273:1058-1073
4. Eggink G, Engel H, Vriend, G, Terpstra P, Witholt B (1990) Rubredoxin reductase of Pseudomonas oleovorans: structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints. J Mol Biol 212:135-142
5. Fujiwara S, Okuyama S, Imanaka T (1996) The world of archaea: genome analysis, evolution and thermostable enzymes. Gene 179:165-170
6. Gosset G, Merino E, Reculas F, Oliver G, Becerril B, Bolivar F (1989) Amino acid sequence analysis of the glutamate synthase enzyme from Escherichia coli K-12. Protein Seq Data Anal 2:9-16
7. Hummelt G, Mora J (1980) Regulation and function of glutamate synthase in Neurospora crassa. Biochem Biophys Res Commun 96:1688-1694
8. Imanaka T, Tanaka T, Tsunekawa H, Aiba S (1981) Cloning of the genes for penicillinase, penP and penI, of Bacillus licheniformis in some vector plasmids and their expression in Escherichia coli, Bacillus subtilis, and Bacillus licheniformis. J Bacteriol 147:776-786
9. Imanaka T, Lee S, Takagi M, Fujiwara S (1995) Aspartyl-tRNA synthetase of hyperthermophilic archaeon Pyrococcus sp. KOD1 has a chimerical structure of eukaryotic and bacterial enzymes. Gene 164:153-156
10. Iwabe N, Kuma K, Hasegawa M, Osawa S, Miyata T (1989) Evolutionary relationship of archaebacteria, eubacteria and eukaryotes inferred from phylogenetic trees of duplicated genes. Proc Natl Acad Sci USA 86:9355-9359
11. + reductase: prototype for a structurally novel flavoenzyme family. Science 251:60-66
12. Knaff DB, Hirasawa M, Ameyibor E, Fu W, Johnson MK (1991) Spectroscopic evidence for a [3Fe-4S] cluster in spinach alulamate synthase. J Biol Chem 266:15080-15084
13. McKie JH, Douglas KT (1991) Evidence for gene duplication forming similar binding folds for NAD(P)H and FAD in pyridine nucleotide-dependent flavoenzymes. FEBS Lett 279:5-8
14. Meers JL, Tempest DW, Brown CM (1970) Glutamine (amide): 2-oxoglutarate amino transferase oxido-reductase (NADP), an enzyme involved in the synthesis of glutamate by some bacteria. J Gen Microbiol 64:187-194
15. Meister A (1985) Glutamate synthase from Escherichia coli, Klebsiella aerogenes, and Saccharomyces cerevisiae. Methods Enzymol 113:327-337
16. Miflin BJ, Lea PJ, Wallsgrove RM (1980) The role of glutamine in ammonium assimilation and reassimilation in plants. In: Mora J, Palacios R (eds) Glutamine: metabolism, enzymology and regulation. Academic Press. New York, pp 213-234
17. Morikawa M, Izawa Y, Rashid N, Hoaki T, Imanaka T (1994) Purification and characterization of a thermostable thiolprotease from a newly isolated hyperthermophilic Pyrococcus sp. Appl Environ Microbiol 60:4559-4566
18. Nagatani H, Shimizu M, Valentine RC (1971) The mechanism of ammonia assimilation in nitrogen-fixing bacteria. Arch Microbiol 79:164-175
19. Oliver G, Gosset G, Sanchez-Pescador R, Lozoya E, Ku LM, Flores N, Becerril B, Valle F, Bolivar F (1987) Determination of the nucleotide sequence for the glutamate synthase structural genes of Escherichia coli K-12. Gene 60:1-11
20. Pelanda R, Vanoni MA, Perego M, Piubelli L, Galizzi A, Curti B, Zanetti G (1993) Glutamate synthase gene of the diazotroph Azospirillum brasilense: cloning, sequencing, and analysis of functional domains. J Biol Chem 268:3099-3106
21. Rivera M, Lake JA (1992) Evidence that eukaryotes and eocyte prokaryotes are immediate relatives. Science 257:74-76
22. Rosenberg AH, Lade BN, Chui DS, Lin SW, Dunn JJ, Studier FW (1987) Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene 56:125-135
23. Sakakibara H, Watanabe M, Hase T, Sugiyama T (1991) Molecular cloning and characterization of complementary DNA encoding for ferredoxin-dependent glutamate synthase in maize leaf. J Biol Chem 266:2028-2035
24. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
25. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467
26. Senior PJ (1975) Regulation of nitrogen metabolism in Escherichia coli and Klebsiella acrogenes: studies with the continuous-culture technique. J Bacteriol 123:407-418
27. Stewart GR, Mann AF, Fentem PA (1980) Glutamate synthase. In: Milling BJ (ed) The biochemistry of plants, vol 5. Academic Press, New York, pp 309-327
28. Studier FW, Mollau BA (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189:113-130
29. Suzuki A, Gadal P (1984) Glutamate synthase: physiochemical and functional properties of different forms in higher plants and in other organisms. Phys Veg 22:471-486
30. Tempest DW, Meers JL, Brown CM (1970) Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route. Biochem J 117:405-407
31. Trotta PP, Platzer KEB, Haschemeyer RH, Meister A (1974) Glutamine-binding subunit of glutamate synthase and partial reactions catalyzed by this glutamine amidotransferase. Proc Natl Acad Sci USA 71:4607-4611
32. Vanoni MA, Edmondson DE, Zanetti G, Curti B (1992) Characterization of the flavins and the iron-sulfur centers of glutamate synthase from Azospririllum brasilense by absorption, circulation dichroism, and electron paramagnetic resonance spectroscopies. Biochemistry 31:4613-4623
33. Wierenga RK, De Maeyer MCH, Hol WGJ (1985) Interaction of pyrophosphate moieties with α-helixes in dinucleotide binding proteins. Biochemistry 24:1346-1357
34. Woese CR, Fox GE (1977) Phylogenetic structure of the prokaryotic domain: the primary kingdoms. Proc Natl Acad Sci USA 74:5088-5090
35. Woese CR, Kandler O, Wheelis ML (1990) Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci USA 87:4576-4579