PCR-single-stranded confirmational polymorphism analysis for non- culture-based subtyping of meningococcal strains in clinical specimens

Journal of Clinical Microbiology

Volumn 35, Issue 7, 1997, Pages 1809-1812

  Newcombe, Jane ^a   Dyer, Steven ^a   Blackwell, Lois ^a,c   Cartwright, Keith ^b   Palmer, Walter H ^b   McFadden, Johnjoe ^a  

^a UNIVERSITY OF SURREY   (United Kingdom)

^b GLOUCESTERSHIRE ROYAL HOSPITAL   (United Kingdom)

^c CBD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM CULTURE; CEREBROSPINAL FLUID CULTURE; EPIDEMIC; HUMAN; INFECTION RATE; MENINGOCOCCOSIS; NEISSERIA MENINGITIDIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEROTYPE; SINGLE STRAND CONFORMATION POLYMORPHISM; STRAIN DIFFERENCE;

EID: 0030919634     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.7.1809-1812.1997     Document Type: Article

References (29)

1. Auriol, J., J. L. Guesdon, M. Guibourdenche, and J. Y. Riou. 1995. Characterization of serogroup A Neisseria meningitidis strains hy rRNA gene restriction patterns and PCR: correlation with the results of serotyping, subtyping and multilocus enzyme electrophoresis. FEMS Immunol. Med. Microbiol. 10:219-226.
2. Bjune, G., E. A. Hoiby, J. K. Gronnesby, et al. 1991. Effect of outer membrane vesicle vaccine against group B meningococcal disease in Norway. Lancet 338:1093-1096.
3. Boom, R., C. J. A. Sol, M. M. M. Salimans, C. L. Jansen, P. M. E. Wertheimvan Dillen, and J. van der Noordaa. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:495-503.
4. Cartwright, K., S. Reilly, D. White, and J. Stuart. 1992. Early treatment with parenteral penicillin in menigococcal disease. Br. Med J. 305:143-147.
5. Cartwright, K. A. V., and D. M. Jones. 1989. Investigation of meningococcal disease. J. Clin. Pathol. 42:634-639.
6. Cartwright, K. A. V., J. M. Stuart, and N. D. Noah. 1986. An outbreak of meningococcal disease in Gloucester. Lancet ii:558-561.
7. Demers, D. B., S. J. Odelberg, and L. M. Fisher. 1990. Identification of a tactor-ix point mutation using SSCP analysis and direct sequencing. Nucleic Acids Res. 18:5575.
8. Feavers, I. M., J. Suker, A. J. McKenna, A. B. Heath, and M. C. Maiden. 1992. Molecular analysis of the serotyping antigens of Neisseria meningitidis. Infect. Immun. 60:3620-3629.
9. Greisen, K., M. Loeffelholz, A. Purohit, and D. Leong. 1994. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J. Clin. Microbiol. 32:335-351.
10. Ilayashi, K. 1991. PCR-SSCP: a simple and sensitive method for detection of mutations in genomic DNA. PCR Methods Appl. 1:34-38.
11. Knight, A. I., K. A. V. Cartwright, and J. J. McFadden. 1990. Identification of a UK outbreak strain of Neisseria meningilidis with a DNA probe. Lancet 335:1182-1184.
12. Kristiansen, B. E., E. Ask, A. Jenkins, C. Fermer, P. Radstrom, and O. Skold. 1991. Rapid diagnosis of meningococcal meningitis by polymerase chain reaction. Lancet 337:1568-1569.
13. Kristiansen, B. E., C. Fermer, A. Jenkins, E. Ask, G. Swedberg, and O. Skold. 1995. PCR amplicon restriction endonuclease analysis of the chromosomal dhps gene of Neisseria meningitidis: a method for studying spread of the disease-causing strain in contacts of patients with meningococcal disease. J. Clin. Microbiol. 33:1174-1179.
14. Maiden, M. C., J. A. Bygraves, J. McCarvil, and I. M. Feavers. 1992. Identification of meningococcal serosubtypes by polymerase chain reaction. J. Clin. Microbiol. 30:2835-2841.
15. Maiden, M. C., J. Suker, A. J. McKenna, J. A. Bygraves, and I. M. Feavers. 1991. Comparison of the class 1 outer membrane proteins of eight serological reference strains of Neisseria meningitidis. Mol. Microbiol. 5:727-736.
16. McGuinness, B., A. K. Barlow, I. N. Clark, J. E. Farley, A. Anilionis, J. T. Poolman, and J. E. Heckels. 1990. Deduced amino acid sequence of class 1 protein (PorA) from three strains of Neisseria meningitidis. J. Exp. Med. 171:1871-1882.
17. Milagres, L. G., S. R. Ramos, C. T. Sacchi, C. E. Melles, V. S. Vieira, H. Sato, G. S. Brito, J. C. Moraes, and C. E. Frasch. 1994. Immune response of Brazilian children to a Neisseria meningitidis serogroup B outer membrane protein vaccine: comparison with efficacy. Infect. Immun. 62:4419-4424.
18. Muralidhar, B., and C. R. Steinman. 1994. Design and characterization of PCR primers for detection of pathogenic neisseriae. Mol. Cell. Probes 8:55-61.
19. Newcombe, J., W. H. Palmer, K. Cartwright, and J. J. McFadden. 1996. PCR of blood specimens for the diagnosis of meningococcal disease. J. Clin. Microbiol. 34:1637-1640.
20. Ni, H., A. I. Knight, K. A. V. Cartwright, and J. J. McFadden. 1992. Phylogenetic and epidemiological analysis of Neisseria meningitidis using DNA probes. Epidemiol. Infect. 109:227-239.
21. Ni, H., A. I. Knight, W. H. Palmer, K. Cartwright, and J. McFadden. 1992. PCR in the diagnosis of meningococcal meningitis. Lancet 340:1432-1434.
22. Rådström, P., A. Bäckman, N. Qian, P. Kragsbjerg, C. Påhlson, and P. Olcén. 1994. Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy. J. Clin. Microbiol. 32: 2738-2744.
23. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd cd. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
24. Saukkonen, K., H. Abdillahi, J. T. Poolman, and M. Leinonen. 1987. Protective efficacy of monoclonal antibodies to class 1 and class 3 outer membrane proteins of Neisseria meningitidis B:15 P1.16 in infant rat infection model; new prospects for vaccine development. Microb. Pathog. 3:261-267.
25. Suker, J., I. M. Feavers, M. Achtman, G. Morelli. J. F. Wang, and M. C. Maiden. 1994. The porA gene in serogroup A meningococci: evolutionary stability and mechanism of genetic variation. Mol. Microbiol. 12:253-265.
26. Telenti, A., P. Imboden, F. Marchesi, D. Lowrie, S. Cole, M. J. Colston, L. Matter, K. Schopfer, and T. Bodmer. 1993. Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis. Lancet 341:647-650.
27. van der Ley, P., and J. T. Poolman. 1992. Construction of a multivalent meningococcal vaccine strain based on the class 1 outer membrane protein. Infect. Immun. 60:3156-3161.
28. Woolford, A. J., and J. W. Dale. 1992. Simplified procedures for detection of amplified DNA using fluorescent label incorporation and reverse probing. FEMS Microbiol. Lett. 99:311-316.
29. Zhu, P., X. Hu, and L. Xu. 1995. Typing Neisseria meningitidis by analysis of restriction fragment length polymorphisms in the gene encoding the class 1 outer membrane protein: application to assessment of epidemics throughout the last 4 decades in China. J. Clin. Microbiol. 33:458-462.