The in vitro phosphorylation of p53 by calcium-dependent protein kinase C characterization of a protein-kinase-C-binding site on p53

European Journal of Biochemistry

Volumn 245, Issue 3, 1997, Pages 684-692

  Delphin, Christian ^a   Huang, Kuo Ping ^b   Scotto, Christian ^c   Chapel, Agnès ^a   Vincon, Mathilde ^a   Chambaz, Edmond ^c   Garin, Jérôme ^a   Baudier, Jacques ^a,b,c  

^a CEA GRENOBLE   (France)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^c INSERM   (France)

Author keywords

DNA binding; p53; protein kinase C

Indexed keywords

PROTEIN KINASE C; PROTEIN P53;

ARTICLE; BINDING SITE; DNA DAMAGE; ENZYME ACTIVITY; MOUSE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN PHOSPHORYLATION; TRANSCRIPTION REGULATION;

MURINAE;

EID: 0030919189     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.t01-1-00684.x     Document Type: Article

References (37)

1. Baudier, J., Deloulme, J. C., Van Dorsselaer, A., Black, D. & Matthes, H. W. D. (1991) Purification and characterization of a brain specific protein kinase C substrate, neurogranin (p17). Identification of a consensus amino acid sequence between neurogranin and neuromodulin (GAP43) that corresponds to the protein kinase C phosphorylation site and the calmodulin-binding domain, J. Biol. Chem. 266, 229-237.
2. Baudier, J., Delphin, C., Grunwald, D., Khochbin, S. & Lawrence, J. J. (1992) Characterization of the tumor suppresser protein p53 as a protein kinase C substrate and a S100B-binding protein, Proc. Natl Acad. Sci. USA 89, 11 627-11 631.
3. Baudier, J., Bergeret, E., Bertacchi, N., Weintraub, H., Gagnon, J. & Garin, J. (1995) Interaction of myogenic bHLH transcription factors with calcium-binding calmodulin and S100 a (αα) proteins, Biochemistry 34, 7834-7846.
4. Caudy, M., Vassin, H., Brand, M., Tuma, R., Jan, L. Y. & Jan, Y. N. (1988) Daughterless, a Drosophila gene essential for both neurogenesis and sex determination, has sequence similarities to myc and the achaete-scute complex, Cell 55, 1061-1067.
5. Chen, S. J., Klann, E., Gower, M. C., Powell, C. M., Sessoms, J. S. & Sweatt, J. D. (1993) Studies with synthetic peptide substrates derived from the neuronal protein neurogranin reveal structural determinants of potency and selectivity for protein kinase C, Biochemistry 32, 1032-1039.
6. Clore, G. M., Omichinski, J. G., Sakaguchi, K., Zambrano, N., Sakamoto, H., Appella, E. & Gronenborn, A. P. (1994) High-resolution structure of the oligomerization domain of p53 by multidimensional NMR, Science 265, 386-390.
7. Delphin, C., Cahen, P., Lawrence, J. J. & Baudier, J. (1994) Characterization of baculovirus recombinant wild-type p53. Dimerization of p53 is required for high-affinity DNA binding and cysteine oxidation inhibits p53 DNA binding, Eur. J. Biochem. 223, 683-692.
8. Delphin, C. & Baudier, J. (1994) The protein kinase C activator, phorbol ester, cooperates with the wild-type p53 species in growth arrest of ras-transformed embryo fibroblasts, J. Biol. Chem. 269, 29 579-29 587.
9. Dulic, V., Kaufmann, W. K., Wilson, S. J., Tlsty, T. D., Lees, E., Harper, J. W., Elledge, S. J. & Reed, S. I. (1994) p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest, Cell 76, 1013-1023.
10. Fields, S. & Jang, S. (1990) Presence of a potent transcription activating sequence in the p53 protein, Science 249, 1046-1049.
11. Graff, J. M., Rajan, R. R., Randall, R. R., Nairn, A. C. & Blackshear, P. J. (1991) Protein kinase C substrate and inhibitor characteristics of peptides derived from the myristoylated alanine-rich C kinase substrate (MARCKS) protein phosphorylation site domain, J. Biol. Chem. 266, 14 390-14 398.
12. Hansen, S., Hupp, T. R. & Lane, D. P. (1996) Allosteric rgulation of the thermostability and DNA binding activity of human p53 by specific interacting protein, J. Biol. Chem. 271, 3917-3924.
13. Hollstein, M., Sidransky, D., Vogelstein, B. & Harris, C. C. (1991) p53 mutations in human cancers, Science 253, 49-53.
14. Houbre, D., Duportail, G., Deloulme, J. C. & Baudier, J. (1991) The interactions of the brain-specific calmodulin-binding protein kinase C substrate, neuromodulin (GAP 43), with membrane phospholipids, J. Biol. Chem. 266, 7121-7131.
15. House, C. & Kemp, B. E. (1987) Protein kinase C contains a pseudosubstrate prototope in its regulatory domain, Science 238, 1726-1728.
16. House, C., Wettenhall, B. H. & Kemp, B. E. (1987) The influence of basic residues on the substrate specificity of protein kinase C, J. Biol. Chem. 262, 772-777.
17. Huang, K.-P. & Huang F. L. (1986) Immunochemical characterization of rat brain protein kinase C, J. Biol. Chem. 261, 14 781-14 787.
18. 2+-activated and phospholipid-dependent protein kinase, Proc. Natl Acad. Sci. USA 83, 8535-8539.
19. Hupp, T. R., Meek, D. W., Midgley, C. A. & Lane, D. P. (1992) Regulation of the specific DNA-binding function of p53, Cell 71, 875-886.
20. Hupp, T. R. & Lane, D. P. (1994) Allosteric activation of latent p53 tetramers, Curr. Biol. 4, 865-875.
21. Hupp, T. R., Sparks, A. & Lane, D. P. (1995). Small peptides activate the latent sequence-specific DNA binding function of p53, Cell, 83, 237-245.
22. James, G. & Olson, E. (1992) Deletion of the regulatory domain of protein kinase C α exposes regions in the hinge and catalytic domains that mediate nuclear targeting, J. Cell Biol. 116, 863-874.
23. Jayaraman, L. & Prives, C. (1995) Activation of p53 sequence-specific DNA binding by short single strands of DNA requires the p53 C-terminus, Cell 81, 1021-1029.
24. Jeffrey, P. D., Gorina, S. & Pavletich, N. (1995) Cristal structure of the tetramerization domain of the p53 tumor supressor at 1.7 Å, Science 267, 1498-1502.
25. Kastan, M. B., Onyekwere, O., Sidransky, D., Vogelstein, B. & Craig, R. W. (1991) Participation of p53 protein in the cellular response to DNA damage, Cancer Rex. 51, 6304-6311.
26. Kim, J., Shishido, T., Jiang, X., Aderem, A. & McLaughlin, S. (1994) Phosphorylation, high ionic strength, and calmodulin reverse the binding of MARCKS to phospholipid vesicles, J. Biol. Chem. 269, 28 214-28 219.
27. Kuerbitz, S. J., Plunkett, B. S., Walsh, W. V. & Kastan, M. B. (1992) Wild-type p53 is a cell cycle checkpoint determinant following irradiation, Proc. Natl Acad Sci. USA 89, 7491-7495.
28. 2+-independent but not Ca2+-dependent protein kinase C isozymes, Biochemistry 34, 3446-3454.
29. Meek, D. W., Simon, S., Kikkawa, U. & Eckhart, W. (1990) The p53 tumor suppressor protein is phosphorylated at serine 389 by casein kinase II, EMBO J. 9, 3253-3260.
30. Milne, D. M., McKendrick, L., Jardine, L. J., Deacon, E., Lord, J. M. & Meek, D. W. (1996) Murine p53 is phosphorylated within the PAb421 epitope by protein kinase C in vitro, but not in vivo, even after stimulation with the phorbol ester o-tetradecanoylphorbol 13-acetate, Oncogene 13, 205-211.
31. Mochly-Rosen, D. (1995) Localization of protein kinases by anchoring proteins: a theme in signal transduction, Science 268, 247-251.
32. Pavletich, N., Chambers, K. A. & Pabo, C. O. (1993) The DNA-binding domain of p53 contains the four conserved regions and the major mutation hot spots, Genes & Dev. 7, 2556-2564.
33. Sakamoto, H., Lewis, M. S., Kodama, H., Appella, E. & Sakaguchi, K. (1994) Specific sequences from the carboxyl terminus of human p53 gene product form anti-parallel tetramers in solution, Proc. Natl Acad. Sci. USA 91, 8974-8978.
34. Shaulian, K., Zauberman, A., Ginsberg, D. & Oren, M. (1992) Identification of a minimal transforming domain of p53: negative dominance through abrogation of sequence-specific DNA binding, Mol. Cell. Biol. 12, 5581-5592.
35. Shaulsky, G., Goldfinger, N., Ben-Zeev, A. & Rotter, V. (1990) Nuclear accumulation of p53 protein is mediated by several nuclear localization signals and plays a role in tumorigenesis, Mol. Cell. Biol. 10, 6565-6577.
36. Shaw, P., Freeman, J., Bovey, R. & Iggo, R. (1995) Regulation of specific DNA binding by p53/evidence for a role for O-glycosylation and charged residues at the carboxy-terminus, Oncogene 12, 921-930.
37. Takenaka, I., Morin, E., Seizinger, B. R. & Kley, N. (1995) Regulation of the sequence-specific DNA binding function of p53 by protein kinase C and protein phosphatase, J. Biol. Chem. 270, 5405-5411.