Comparative study of the damage produced by acute ethanol and acetaldehyde treatment in a human fetal hepatic cell line

Toxicology

Volumn 120, Issue 2, 1997, Pages 133-144

  Olivares, I P ^a   Bucio, L ^a   Souza, V ^a   Carabez A ^b   Gutierrez Ruiz M C ^a  

^a DEPARTAMENTO DE QUÍMICA   (Mexico)

^b UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

Author keywords

Acetaldehyde; Ethanol; Hepatic human cell culture; WRL 68

Indexed keywords

ACETALDEHYDE; ALCOHOL; GLUTATHIONE; LACTATE DEHYDROGENASE;

ARTICLE; CELL ADHESION; CELL RESPIRATION; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; FETUS; FETUS CELL; HUMAN; HUMAN CELL; LIPID PEROXIDATION; LIVER CELL CULTURE; LIVER TOXICITY; PRIORITY JOURNAL; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ACETALDEHYDE; ANALYSIS OF VARIANCE; CELL ADHESION; CELL DIVISION; CELL LINE; CELL RESPIRATION; CELL SURVIVAL; ETHANOL; GLUTATHIONE; HUMANS; L-LACTATE DEHYDROGENASE; LIPID PEROXIDATION; LIVER; LYSOSOMES; MICROSCOPY, ELECTRON; MITOCHONDRIA, LIVER; NEUTRAL RED; OXYGEN CONSUMPTION; SOLVENTS;

ANGUILLIFORMES;

EID: 0030918232     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-483X(97)03650-0     Document Type: Article

References (33)

1. Alley, M.C., Scudeiro, D.A., Monks, A., Hursey, M.L., Czerwinsk, M.J., Fine, D.L., Abbott, B.J., Mayo, J.G., Shoemaker, R.H. and Boyd, M.R. (1988) Feasibility of drug screening with panels of human tumor cell lines using microculture tetrazolium assay. Cancer Res. 48, 589-601.
2. Bondy, S.C. (1992) Ethanol toxicity and oxidative stress. Toxicol. Lett. 63, 231-241.
3. Borenfreund, E. and Puerner, J.A. (1986) Cytotoxicity of metals, metal-metal and metal-chelator combinations assayed in vitro. Toxicology 39, 121-134.
4. Buege, J.A. and Aust, S.D. (1978) Microsomal lipid peroxidation. Methods Enzymol. 52, 302-310.
5. Burkart, V., Koike, T., Brenner, H.H. and Kolb, H. (1992) Oxygen radicals generated by the enzyme xanthine oxidase lyse rat pancreatic islet cells in vitro. Diabetologia 35, 1028-1034.
6. Chen, L.H., Hu, N. and Huang, T.L. (1992) Effects of acute alcohol intoxication on liver antioxidant defense systems in rats. Biochem. Arch. 8, 95-100.
7. Cook, R-T. and Martin, G.S. (1986) Clonal response to ethanol of proliferating rat hepatoma cells in vitro. Alcohol. Clin. Exp. Res. 10, 16-21.
8. Devi, B.G., Henderson, G.I., Frosto, T.A. and Schenker, S. (1993) Effect of ethanol on rat fetal hepatocytes: studies on cell replication, lipid peroxidation and glutathione. Hepatology 3, 648-658.
9. Devi, B.G., Henderson, G.I., Frosto, T.A. and Schenker, S. (1994) Effect of acute ethanol exposure on cultured fetal rat hepatocytes: relation to mitochondrial function. Alcohol. Clin. Exp. Res. 18, 1436-1442.
10. Ellman, G.L. (1959) Tissue sulfhydryl groups. Arch. Biochem. 82, 70.
11. Farbiszewski, R., Chwiecko, M., Holownia, A. and Brzozowski, M. (1988) Changes in sulfhydryl compounds in plasma, liver and brain after acute and chronic acetaldehyde administration in rats. Drug Alcohol Depend. 22, 79-82.
12. Gonzalez, J., Muñoz, M.E., Martin, M.I., Collado, P.S., Fermoso, J. and Esteller, A. (1988) Influence of acute ethanol administration on hepatic glutathione metabolism in the rat. Alcohol 5, 103-106.
13. + transport systems on WRL-68 cells. Hum. Exp. Toxicol. 14, 324-334.
14. Gutiérrez-Ruiz, C., Bucio, L., Souza, V., Gómez, J.J. and Cárabez, A. (1994) Expression of some hepatocyte-like functional properties of WRL-68 cells in culture. In Vitro Cell. Dev. Biol. 30A, 366-371.
15. Higgins, P.J. and Borenfreud, E. (1986) Incubation of rat hepatic tumor cells with ethanol and acetaldehyde in vitro: effects on growth rate, albumin secretion and cellular protein content. Digestion 34, 161-168.
16. Hoffmann, T., Meyer, R.J., Sorrell, M.F. and Tuma, D.J. (1993) Reaction of acetaldehyde with proteins: formation of stable fluorescent adducts. Alcohol. Clin. Exp. Res. I, 69-74.
17. Johannisson, A., Thuvander, A. and Gadhasson, I.L. (1995) Activation markers and cell proliferation as indicators of toxicity: a flow cytometric approach. Cell Biol. Toxicol. 11, 355-366.
18. Kawase, T., Kato, S. and Lieber, C.S. (1989) Lipid peroxidation and antioxidant defense systems in rat liver after chronic ethanol feeding. Hepatology 5, 815-821.
19. Lieber, C.S. (1991) Hepatic, metabolic and toxic effects of ethanol. Alcohol. Clin. Exp. Res. 15, 573-592.
20. Lindros, K.O. (1995) Alcoholic liver disease: pathobiological aspects. J. Hepatol. 23, 7-15.
21. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the Folin-phenol reagent. J. Biol. Chem. 193, 265-275.
22. Majchrowicz, E. (1975) Induction of physical dependence upon ethanol and the associated behavioral changes in rats. Psychopharmacologia (Berl.) 43, 245-254.
23. Mapoles, J.E., Iwahashi, M., Lucas D., Zimmerman, B.T. and Simon, F.R. (1994) Acetaldehyde exposure causes growth inhibition in a Chinese hamster ovary cell line that expresses alcohol dehydrogenase. Alcohol. Clin. Exp. Res. 3, 632-639.
24. Martensson, J.A., Jain, A., Stole, E., Frayer, W., Auld, P.A.M: and Meister, A. (1991) Inhibition of glutathione synthesis in newborn: a model for endogenously produced oxidative stress. Proc. Natl. Acad. Sci. USA 88, 9360-9364.
25. Mithofer, K., Sandy, M.S., Smith, M.T. and Di Monte, D. (1992) Mitochondrial poisons cause depletion of reduced glutathione in isolated hepatocytes. Arch. Biochem. Biophys. 295, 132-136.
26. Moldeus, P., Hogberg, J. and Orrenius, S. (1978) Isolation and use of liver cells. In: W.B. Jacoby (Ed), Methods in Enzymology, pp. 60-71.
27. Sadrzadeh, S.M.H. and Nanji, A.A. (1994) Detection of lipid peroxidation after acute ethanol administration is dependent on time of sampling. Int. J. Vit. Nutr. Res. 64, 157-158.
28. Speisky, H., Bunout, D., Orrego, H., Giles, H.G., Gunasekara, A. and Israel, Y. (1985) Lack of changes in diene conjugate levels following ethanol induced glutathione depletion or hepatic necrosis. Res. Commun. Chem. Pathol. Pharmacol. 48, 77-89.
29. Uysal, M., Özdemirler, G., Kutalp, G. and Öz, H. (1989) Mitochondrial and microsomal lipid peroxidation in rat liver after acute acetaldehyde and ethanol intoxication. J. Appl. Toxicol. 3, 155-158.
30. Videla, L.A., Fernandez, V. and De Marinis, A. (1982) Liver lipoperoxidative pressure and glutathione status following acetaldehyde and aliphatic alcohols pretreatments in the rat. Biochem. Biophys. Res. Commun. 104, 965-970.
31. Videla, L.A., Fernandez, V., Ugorete, G. and Valenzuela, A. (1980) Effect of acute ethanol intoxication on the content of reduced glutathione of the liver in relation to its lipoperoxidative capacity in the rat. FEBS Lett. 111, 6-10.
32. Viña, J., Estrela, J.M., Guerri, C. and Romero, F.J. (1980) Effect of ethanol on glutathione concentration in isolated hepatocytes. Biochem. J. 188, 549-552.
33. Wlodek, L. and Rommelspacher, H. (1994) Ethanol-induced changes in the content of thiol compound and of lipid peroxidation in livers and brains from mice: protection by thiazolidine derivatives. Alcohol Alcohol. 6, 649-657.