Characterization and purification from bovine neutrophils of a soluble guanine-nucleotide-binding protein that mediates isozyme-specific stimulation of phospholipase C β2

European Journal of Biochemistry

Volumn 246, Issue 1, 1997, Pages 71-77

  Illenberger, Daria ^a   Schwald, Frieder ^a   Gierschik, Peter ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

Guanine nucleotide binding regulatory protein; Low molecular mass GTP binding protein; Neutrophil; Phosphatidylinositol phospholipase C; Signal transduction

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; PHOSPHOLIPASE C;

ANIMAL CELL; ARTICLE; CATTLE; CELL STRAIN HL 60; CONTROLLED STUDY; CYTOSOL; DELETION MUTANT; ENZYME ACTIVATION; ENZYME ACTIVITY; GEL FILTRATION; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR WEIGHT; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION;

EID: 0030917334     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.t01-1-00071.x     Document Type: Article

References (35)

1. Berridge, M. J. (1993) Inositol trisphosphate and calcium signalling. Nature 361, 315-325.
2. Dekker, L. V., Palmer, R. H. & Parker, P. J. (1995) The protein kinase C and protein kinase C-related gene families, Curr. Opin. Struct. Biol. 5, 396-402.
3. Noh, D.-Y., Shin, S. H. & Rhee, S. G. (1995) Phosphoinositide-specific phospholipase C and mitogenic signaling, Biochim. Biophys. Acta 1242, 99-114.
4. Camps, M., Hou, C., Jakobs, K. H. & Gierschik, P. (1990) Guanosine 5′-[γ-thio]triphosphate-stimulated hydrolysis of phosphatidylinositol 4,5-bisphosphate in HL-60 granulocytes: evidence that the guanine nucleotide acts by relieving phospholipase C from an inhibitory constraint, Biochem. J. 271, 743-748.
5. Camps, M., Hou, C., Sidiropoulos, D., Stock, J. B., Jakobs, K. H. & Gierschik, P. (1992) Stimulation of phospholipase C by G-protein βγ-subunits, Eur. J. Biochem. 206, 821-831.
6. 2 by G-protein βγ-subunits, Nature 360, 684-686.
7. Carozzi, A., Camps, M, Gierschik, P. & Parker, P. J. (1993) Activation of phosphatidylinositol lipid-specific phospholipase C-β3 by G-protein βγ subunits, FEBS Lett. 315, 340-342.
8. Gierschik, P. & Camps, M. (1994) Stimulation of phospholipase C by G-protein βγ subunits, Methods Enzymol. 238, 181-195.
9. Simoes, A. P., Schnabel, P., Pipkorn, R., Camps, M. & Gierschik, P. (1993) A peptide corresponding to a potential polyphosphoinositide binding site of phospholipase C-β2 enhances its catalytic activity, FEBS Lett. 331, 248-251.
10. Gierschik, P, Sidiropoulos, D., Spiegel, A. M. & Jakobs, K. H. (1987) Purification and immunochemical characterization of the major pertussis-toxin-sensitive guanine-nucleotide-binding protein of bovine-neutrophil membranes, Eur. J. Biochem. 165, 185-194.
11. Sternweis, P. C. & Robishaw, J. D. (1984) Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain, J. Biol. Chem. 259, 13806-13813.
12. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 248-254.
13. Carozzi, A., Kriz, R. W., Webster, C. & Parker, P. J. (1992) Identification, purification and characterization of a novel phosphatidylinositol-specific phospholipase C: a third member of the β subfamily, Eur. J. Biochem. 210, 521-529.
14. Pennock, G. D., Shoemaker, C. & Miller, L. K. (1984) Strong and regulated expression of Escherichia coli β-galactosidase in insect cells with a baculovirus vector, Mol. Cell. Biol. 4, 399-406.
15. 1 requires a pleckstrin homology domain for interaction with the plasma membrane, Biochem. J. 312, 661-666.
16. Stephens, L., Jackson, T. R. & Hawkins, P. T. (1993) Activation of phosphatidylinositol 4,5-bisphsphate supply by agonists and nonhydrolysable GTP analogues, Biochem. J. 296, 481-488.
17. Chong, L. D., Traynor-Kaplan, A., Bokoch, G. M. & Schwartz, A. M. (1994) The small GTP-binding protein Rho regulates a phosphatidylinositol 4-phosphate 5-kinase in mammalian cells, Cell 79, 507-513.
18. Hartwig, J. H., Bokoch, G. M., Carpenter, C. L., Janmey, P. A., Taylor, L. A., Token A. & Stossel, T. P. (1995) Thrombin receptor ligation and activated rac uncap actin filament barbed ends through phosphoinositide synthesis in permeabilized human platelets, Cell 82, 643-653.
19. Baldassare, J. J. & Fisher, G. J. (1986) Regulation of membrane-associated and cytosolic phospholipase C activities in human platelets by guanosine triphosphate, J. Biol. Chem. 261, 11942-11944.
20. Deckmyn, H., Tu, S.-M. & Majerus, P. W. (1986) Guanine nucleotides stimulate soluble phosphoinositide-specific phospholipase C in the absence of membranes, J. Biol. Chem. 261, 16553-16558.
21. Fisher, G. J., Baldassare, J. J. & Voorhees, J. J. (1989) GTP-dependent hydrolysis of phosphatidylinositol-4,5-bisphosphate by soluble phospholipase C from adult human epidermis, J. Invest. Dermatol. 92, 831-836.
22. Wang, P., Toyoshima, S. & Osawa, T. (1988) Concanavalin A-induced translocation of part of GTP binding actvity from the membrane to the cytosol in murine thymocytes, J. Biochem. 104, 169-172.
23. Ryu, S. H., Cho, K. S., Lee, K.-Y., Sun, P.-G. & Rhee, S. G. (1987) Purification and characterization of two immunologically distinct phosphoinositide-specific phospholipase C from bovine brain, J. Biol. Chem. 262, 12511-12518.
24. q, J. Biol. Chem. 269, 25335-25338.
25. Park, D., Jhon, D.-Y., Lee, C.-W., Ryu, S. H. & Rhee, S. G. (1993) Removal of the carboxyl-terminal region of phospholipase C-β1 by calpain abolishes activation by Gαq, J. Biol. Chem. 268, 3710-3714.
26. Wu, D., Jiang, H., Katz, A. & Simon, M. I. (1993) Identification of critical regions on phospholipase C-β1 required for activation by G-protein, J. Biol. Chem. 268, 3704-3709.
27. q and βγ subunits, J. Biol. Chem. 268, 25952-25957.
28. Wu, D., Katz, A. & Simon, M. I. (1993) Activation of phospholipase C β2 by the α and βγ subunits of trimeric GTP-binding proteins, Proc. Natl Acad. Sci. USA 90, 5297-5301.
29. Banno, Y., Asano, T. & Nozawa, Y. (1994) Proteolytic modification of membrane-associated phospholipase C-β by μ-calpain enhances its activation by G-protein βγ subunits in human platelets, FEBS Lett. 340, 185-188.
30. Banno, Y., Nakashima, S., Hachiya, T. & Nozawa, Y. (1995) Endogenous cleavage of phospholipase C-β3 by agonist-induced activation of calpain in human platelets, J. Biol. Chem. 270, 4318-4324.
31. 1-coupled receptor signaling, J. Biol. Chem. 270, 12984-12989.
32. Kahn, R. A. (1991) Fluoride is not an activator of the smaller (20-25kDa) GTP-binding proteins, J. Biol. Chem. 266, 15595-15597.
33. Bourne, H. R., Sanders, D. A. & McCormick, F. (1990) The GTPase superfamily: a conserved switch for diverse cell functions, Nature 348, 125-132.
34. Baldassare, J. J., Knipp, M. A., Hendersson, P. A. & Fisher, G. J. (1988) GTPγS-stimulated hydrolysis of phosphatidylinositol-4,5-bisphosphate by soluble phospholipase C from human platelets requires soluble GTP-binding protein, Biochem. Biophys. Res. Commun. 154, 351-357.
35. Boguski, M. S. & McCormick, F. (1993) Proteins regulating Ras and its relatives, Nature 366, 643-654.