Flow cytometry, sorting and immunocharacterization with proliferating cell nuclear antigen of cycling and non-cycling cells in synchronized pea root tips

Planta

Volumn 202, Issue 2, 1997, Pages 188-195

  Onelli, Elisabetta ^a   Citterio, Sandra ^a   O'Connor, Jose' Enrique ^b   Levi, Marisa ^c   Sgorbati, Sergio ^a  

^a CNR   (Italy)

^b UNIVERSITY OF VALENCIA   (Spain)

^c UNIVERSITY OF VERONA   (Italy)

Author keywords

Cell cycle (root); Flow cytometry and sorting Pisum; Proliferating cell nuclear antigen (immunolocalization); Root meristem (synchronization)

Indexed keywords

CYCLINE; HYDROXYUREA;

ARTICLE; CELL CYCLE; CELL NUCLEUS; CYTOLOGY; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; MERISTEM; METABOLISM; MICROTOMY; PEA; PLANT ROOT;

CELL CYCLE; CELL NUCLEUS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HYDROXYUREA; MERISTEM; MICROTOMY; PEAS; PLANT ROOTS; PROLIFERATING CELL NUCLEAR ANTIGEN;

ANIMALIA; PISUM; PISUM SATIVUM; SATIVUM;

EID: 0030916867     PISSN: 00320935     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004250050118     Document Type: Article

References (17)

1. Barlow PW (1978) The concept of the stem cell in the context of plant growth and development. In: Potten CS, Cole RI (eds) Stem cells and tissue homeostasis. Cambridge University Press, Cambridge, pp 87-113
2. Barlow PW (1992) The cell division cycle in relation to root organogenesis. In: Omrod JC, Francis D (eds) Molecular and cell biology of the plant cell cycle. Kluwer, Dordrecht, The Netherlands, pp 179-199
3. Bravo R, Macdonald-Bravo H (1987) Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites. J Cell Biol 105: 1549-1554
4. Citterio S, Sgorbati S, Levi M, Colombo BM, Sparvoli E (1992) PCNA and total nuclear protein content as markers of cell proliferation in pea tissue. J Cell Sci 102: 71-78
5. Darzynkiewicz Z, Crissman HA (1990) Flow cytometry. Methods Cell Biol 33, Academic Press, San Diego
6. Darzynkiewicz Z, Traganos F, Kimmel M (1987) Assay of cell cycle kinetics by multivariate flow cytometry using the principle of stathmokinesis. In: Gray GW, Darzynkiewicz Z (eds) Techniques in cell cycle analysis. Humana, Clifton, pp 291-336
7. Evans LS, Van't Hof J (1975) The age-distribution of cell cycle populations in plant root meristems. Exp Cell Res 90: 401-410
8. Hall PA, Woods AL (1990) Immunohistochemical markers of cellular proliferation: achievements, problems and prospects. Cell Tiss Kinet 23: 505-522
9. Morris GF, Mathews MB (1989) Regulation of proliferating cell nuclear antigen during cell cycle. J Biol Chem 264: 13856-13864
10. Sabelli PA, Burgess SR, Carbajosa JV, Parker JS, Halford NG, Shewry PR, Barlow PW (1992) Molecular characterization of cell populations in the maize root apex. In: Omrod JC, Francis D (eds) Molecular and cell biology of the plant cell cycle. Kluwer, Dordrecht, The Netherlands, pp 97-109
11. Sgorbati S, Levi M, Sparvoli E, Trezzi F, Lucchini G (1986) Cytometry and flow cytometry of 4′,6-diamidino-2-phenylindole (DAPI)-stained suspensions of nuclei released from fresh and fixed tissues of plants. Physiol Plant 68: 471-476
12. Sgorbati S, Sparvoli E, Levi M, Galli MG, Citterio S, Chiatante D (1991) Cell cycle kinetic analysis with flow cytometry in pea root meristem synchronized with aphidicolin. Physiol Plant 81: 507-512
13. Sgorbati S, Citterio S, Sparvoli E (1994) Cell cycle kinetic analysis with bivariate flow cytometry of DNA and nuclear protein in synchronized pea root meristem. In: Kimler BF, O'Connor JE, Sansonetty F, Brown SC (eds) The Porto workshop on flow and image cytometry: a collection of ideas. Dacao Eng. Antonio de Almeida, Porto, pp 15-23
14. Steeves TA, Sussex IM (1989) Patterns in plant development. 2nd edn, Cambridge University Press, Cambridge, New York
15. Van't Hof J (1974) The duration of chromosomal DNA synthesis, of the mitotic cycle, and of meiosis of higher plants. In: King RC (ed) Handbook of genetics, vol. 2. Plenum Press, New York, pp 363-377
16. Waseem NH, Lane DP (1990) Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). J Cell Sci 96: 121-129
17. Yu CCW, Woods AL, Levison DA (1992) The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications. Histochem J 24: 121-131