Microplate digestion of minipreps from a single microcentrifuge tube

BioTechniques

Volumn 22, Issue 5, 1997, Pages 870-874

  Brady, Jamie L ^a   Lew, Andrew M ^a,b  

^a Water and Eliza Hall Institute   (Australia)

^b ROYAL MELBOURNE HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CENTRIFUGATION; DNA DETERMINATION; MICROANALYSIS; NONHUMAN; PLASMID;

EID: 0030916049     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97225bm19     Document Type: Article

References (5)

1. Holmes, D.S. and M. Quigley. 1981. A rapid boiling method for preparation of bacterial plasmids. Anal. Biochem. 114:193-197.
2. Liu, Z. and N.C. Mishra. 1995. Single-tube method for plasmid miniprep from large numbers of clones for direct screening by size or restriction digestion. BioTechniques 18:214-217.
3. Majumdar, M.K. and D.A. Williams. 1992 Minipreparations of plasmid DNA directly from cell culture by the boiling method. BioTechniques 13:366.
4. Rajeevan, M.S. and C.L. Bassett. 1994. Flexibility in the boiling method of extracting plasmid DNA directly from cell culture. BioTechniques 16:376-380.
5. Xiang, C., H. Wang, P. Shiel, P. Berger and D.J. Guerra. 1994. A modified alkaline lysis miniprep protocol using a single microcentrifuge tube. BioTechniques 17:30-32.