Ndj1p, a meiotic telomere protein required for normal chromosome synapsis and segregation in yeast

Science

Volumn 276, Issue 5316, 1997, Pages 1252-1255

  Conrad, Michael N ^a   Dominguez, Ana M ^a   Dresser, Michael E ^a  

^a OKLAHOMA MEDICAL RESEARCH FOUNDATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CHROMOSOME PAIRING; CROSSING OVER; GENE SEGREGATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; SACCHAROMYCES CEREVISIAE;

CHROMOSOMES, ARTIFICIAL, YEAST; FUNGAL PROTEINS; MEIOSIS; NONDISJUNCTION, GENETIC; NUCLEAR PROTEINS; SACCHAROMYCES CEREVISIAE; SYNAPTONEMAL COMPLEX; TELOMERE;

EID: 0030915814     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5316.1252     Document Type: Article

References (27)

1. r spores. MCY387 was transformed with a library of wild-type yeast genomic DNA fragments in the URA3, leu2-d vector pHR81 [J. O. Nehlin, M. Carlberg, H. Ronne, Gene 85, 313 (1989)], and plasmid copy number was controlled by selection for URA3 or LEU2. Plasmids that interfered with meiosis or sporulation were subjected to transposon mutagenesis [M. Strathman et al., Proc. Natl. Acad. Sci. U.S.A. 88, 1247 (1991)] to map the region responsible for the meiotic effect. The NDJ1 open reading frame was identified in three plasmids that caused increased nondisjunction with URA3 selection (∼30 copies per cell) and prevented sporulation with LEU2 selection (∼100 copies per cell).
2. r spores. MCY387 was transformed with a library of wild-type yeast genomic DNA fragments in the URA3, leu2-d vector pHR81 [J. O. Nehlin, M. Carlberg, H. Ronne, Gene 85, 313 (1989)], and plasmid copy number was controlled by selection for URA3 or LEU2. Plasmids that interfered with meiosis or sporulation were subjected to transposon mutagenesis [M. Strathman et al., Proc. Natl. Acad. Sci. U.S.A. 88, 1247 (1991)] to map the region responsible for the meiotic effect. The NDJ1 open reading frame was identified in three plasmids that caused increased nondisjunction with URA3 selection (∼30 copies per cell) and prevented sporulation with LEU2 selection (∼100 copies per cell).
3. M. Vandenbol et al., Yeast 11, 1069 (1995).
4. N. Burns et al., Genes Dev. 8, 1087 (1994).
5. M. N. Conrad, A. M. Dominguez, M. E. Dresser, unpublished results.
6. M. Goldway, T. Arbel, G. Simchen, Genetics 133, 149 (1993); M. Flatters and D. Dawson, ibid. 134, 423 (1993).
7. M. Goldway, T. Arbel, G. Simchen, Genetics 133, 149 (1993); M. Flatters and D. Dawson, ibid. 134, 423 (1993).
8. R. S. Hawley, in Genetic Recombination, R. Kucherlapati and G. R. Smith, Eds. (American Society for Microbiology, Washington, DC, 1988), pp. 497-528.
9. T. D. Petes, R. E. Malone, L. S. Symington, in The Molecular and Cellular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein Synthesis, and Energetics, J. R. Broach, J. R. Pringle, E. W. Jones, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, ed. 2, 1991), vol. 1, pp. 407-521.
10. D. D. Sears, J. H. Hegemann, P. Hieter, Proc. Natl. Acad. Sci. U.S.A. 89, 5296 (1992).
11. D. D. Sears, P. Hieter, G. Simchen, Genetics 138, 1055 (1994).
12. L. Clarke and J. Carbon, Nature 287, 504 (1980); C. Mann and R. W. Davis, Proc. Natl. Acad. Sci. U.S.A. 83, 6017 (1986); D. S. Dawson, A. W. Murray, J. W. Szostak, Science 234, 713 (1986); T. Nilsson-Tillgren et al., Carlsberg Res. Commun. 51, 309 (1986); D. B. Kaback, Curr. Genet. 15, 385 (1989); J. Loidl, H. Scherthan, D. B. Kaback, Proc. Natl. Acad. Sci. U.S.A. 91, 331 (1994).
13. L. Clarke and J. Carbon, Nature 287, 504 (1980); C. Mann and R. W. Davis, Proc. Natl. Acad. Sci. U.S.A. 83, 6017 (1986); D. S. Dawson, A. W. Murray, J. W. Szostak, Science 234, 713 (1986); T. Nilsson-Tillgren et al., Carlsberg Res. Commun. 51, 309 (1986); D. B. Kaback, Curr. Genet. 15, 385 (1989); J. Loidl, H. Scherthan, D. B. Kaback, Proc. Natl. Acad. Sci. U.S.A. 91, 331 (1994).
14. L. Clarke and J. Carbon, Nature 287, 504 (1980); C. Mann and R. W. Davis, Proc. Natl. Acad. Sci. U.S.A. 83, 6017 (1986); D. S. Dawson, A. W. Murray, J. W. Szostak, Science 234, 713 (1986); T. Nilsson-Tillgren et al., Carlsberg Res. Commun. 51, 309 (1986); D. B. Kaback, Curr. Genet. 15, 385 (1989); J. Loidl, H. Scherthan, D. B. Kaback, Proc. Natl. Acad. Sci. U.S.A. 91, 331 (1994).
15. L. Clarke and J. Carbon, Nature 287, 504 (1980); C. Mann and R. W. Davis, Proc. Natl. Acad. Sci. U.S.A. 83, 6017 (1986); D. S. Dawson, A. W. Murray, J. W. Szostak, Science 234, 713 (1986); T. Nilsson-Tillgren et al., Carlsberg Res. Commun. 51, 309 (1986); D. B. Kaback, Curr. Genet. 15, 385 (1989); J. Loidl, H. Scherthan, D. B. Kaback, Proc. Natl. Acad. Sci. U.S.A. 91, 331 (1994).
16. L. Clarke and J. Carbon, Nature 287, 504 (1980); C. Mann and R. W. Davis, Proc. Natl. Acad. Sci. U.S.A. 83, 6017 (1986); D. S. Dawson, A. W. Murray, J. W. Szostak, Science 234, 713 (1986); T. Nilsson-Tillgren et al., Carlsberg Res. Commun. 51, 309 (1986); D. B. Kaback, Curr. Genet. 15, 385 (1989); J. Loidl, H. Scherthan, D. B. Kaback, Proc. Natl. Acad. Sci. U.S.A. 91, 331 (1994).
17. L. Clarke and J. Carbon, Nature 287, 504 (1980); C. Mann and R. W. Davis, Proc. Natl. Acad. Sci. U.S.A. 83, 6017 (1986); D. S. Dawson, A. W. Murray, J. W. Szostak, Science 234, 713 (1986); T. Nilsson-Tillgren et al., Carlsberg Res. Commun. 51, 309 (1986); D. B. Kaback, Curr. Genet. 15, 385 (1989); J. Loidl, H. Scherthan, D. B. Kaback, Proc. Natl. Acad. Sci. U.S.A. 91, 331 (1994).
18. M. E. Dresser and C. N. Giroux, J. Cell biol. 106, 567 (1988).
19. F. Klein et al., ibid. 117, 935 (1992).
20. M. Gotta et al., ibid. 134, 1349 (1996).
21. B. M. Weiner and N. Kleckner, Cell 77, 977 (1994).
22. M. P. Maguire, Exp. Cell Res. 112, 297 (1978).
23. K. E. Kirk, B. P. Harmon, I. K. Reichardt, J. W. Sedat, E. H. Blackburn, Science 275, 1478 (1997).
24. M. J. Moses, Annu. Rev. Genet. 2, 363 (1968).
25. M. E. Dresser et al., Genetics 138, 633 (1994).
26. D. D. Perkins, ibid. 34, 607 (1949).
27. We thank C. Barton, D. Goody, D. Ewing, and J. Hulen for technical assistance; P. Hieter, H. Ronne, and K. Runge for strains and plasmids; J. Berman for antibodies to Rap1p; P. Silverman for reading the manuscript; and the core facility at the University of Oklahoma Health Sciences Center for oligonucleotides. Supported by grants GM45250 from NIH to M.E.D., HN2-034 from the Oklahoma Center for the Advancement of Science and Technology to M.N.C., and MCB-9507089 from NSF to M.E.D. and M.N.C.