Double-labeled fluorescent probes for 5' nuclease assays: Purification and performance evaluation

BioTechniques

Volumn 22, Issue 6, 1997, Pages 1140-1145

  Rudert, W A ^a   Braun, E R ^a   Faas, S J ^a   Menon, R ^a   Jaquins Gerstl, A ^a   Trucco, M ^a,b  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b CHILDREN S HOSPITAL OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; NUCLEASE;

ALLELE; ARTICLE; DNA DETERMINATION; DNA PROBE; GENE AMPLIFICATION; GENE SEQUENCE; GENE TARGETING; PURIFICATION;

EID: 0030914896     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97226rr02     Document Type: Article

References (10)

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2. Holland P.M., R.D. Abramson, R. Watson and D.H. Gelfand. 1991. Detection of specific polymerase chain reaction product by utilizing the 5′ to 3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88:7276-7280.
3. Innis, M.A., K.B. Myambo, D.H. Gelfand and M.D. Brow. 1988. DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Proc. Natl. Acad. Sci. USA 85:9436-9440.
4. Jordan, J.A., S.J. Faas, E.R. Braun and M. Trucco. 1996. Exonuclease-released fluorescence detection of human parvovirus B19 DNA. Mol. Diag. 1:321-328.
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6. Livak, K.J., S.J.A. Flood, J. Marmaro, W. Giusti and K. Deetz. 1995. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. 4:1-6.
7. Livak, K.J., J. Marmaro and S. Flood. 1995. Guidelines for designing Taqman fluorescent probes for 5′ nuclease assays. Research News. PE Applied Biosystems, Foster City, CA.
8. Livak, K.J., J. Marmaro and J. Todd. 1995. Towards fully automated genome-wide polymorphism screening. Nature Genet. 9:341-342.
9. Menon, R., W.A. Rudert, E.R. Braun, A. Jaquins-Gerstl, S.J. Faas and M. Trucco. 1997. Sequence-specific priming and exonuclease-released fluorescence assay for rapid and reliable HLA-A molecular typing. Mol. Diag. 2:1-13.
10. Olerup, O. and H. Zetterquist. 1992. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 39:225-215.