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Because this number is obtained from a steady-state measurement, which does not discriminate against the 2-ns impurity or degradation product (Figure 7a), it overestimates the fluorescence quantum yield of the analog. The decrease in fluorescence quantum yield of the analog is more clearly apparent in the decay of its excited singlet state: in DMSO, it possesses a fluorescence decay of 500 ps, as opposed to 5 ns for that of hypericin (Figure 7). Consequently, a large component of the triplet population in hexamethoxyhypericin is expected to appear with a 500 ps time constant, as is observed in Figure 6.
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