Polyphenoloxidases from Williams Pear (Pyrus communis L, cv Williams): Activation, purification and some properties

Journal of the Science of Food and Agriculture

Volumn 74, Issue 1, 1997, Pages 49-56

  Gauillard, Frédéric ^a   Richard Forget, Florence ^a  

^a CEMAGREF   (France)

Author keywords

activation; enzymatic browning; pear; polyphenoloxidase; purification

Indexed keywords

PYRUS COMMUNIS;

EID: 0030912625     PISSN: 00225142     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0010(199705)74:1<49::AID-JSFA769>3.0.CO;2-K     Document Type: Article

References (33)

1. Amiot M J, Tacchini M, Aubert S, Oleszek W 1995 Influence of cultivar, maturity stage, and storage conditions on phenolic composition and enzymatic browning of pear fruits. J Agric Food Chem 43 1132-1137.
2. Angleton E L, Flurkey W H 1984 Activation and alteration of plant and fungal polyphenol oxidase isoenzymes in sodium dodecylsulfate electrophoresis. Phytochemistry 23 2723-2725.
3. Asaka M, Aoyama Y, Nakanishi R, Hayashi R 1994 Purification of a latent form of polyphenoloxidase from La France pear fruit and its pressure-activation. Biosci Biotechnol Biochem 58 1486-1489.
4. Aspan A, Huang T, Cerenius L, Söderhäll K 1995 cDNA cloning of prophenoloxidase from freshwater crayfish Pacifastacus leniusculus and its activation. Proc Natl Acad Sci 92 939-943.
5. Bradford M M 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72 248-254.
6. Fils B, Sauvage X, Nicolas J 1985 Tomato peroxidases. Purification and some properties. Sci Alim 5 217-232.
7. Fraignier M P, Marques L, Fleuriet A, Macheix J J 1995 Biochemical and immunochemical characteristics of Polyphenol oxidases from different fruits of Prunus. J Agric Food Chem 43 2375-2380.
8. Golbeck J H, Cammarata KV 1981 Spinach thylakoid polyphenol oxidase. Plant Physiol 67 977-984.
9. Halim D H, Montgomery MW 1978 Polyphenol oxidase of d'Anjou pears (pyrus communis L). J Food Sci 43 603-608.
10. Kahn V 1977 Effects of proteins, protein hydrolyzates and amino acids on o-dihydroxyphenolase activity of polyphenol oxidase of mushroom, avocado and banana. J Food Sci 50 111-119.
11. King R S, Flurkey WH 1987 Effects of limited proteolysis on broad bean polyphenol oxidase. J Sci Food Agric 41 231-240.
12. Laemmli U K 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227 680-685.
13. Lerner H R, Mayer AM 1975 Stoke's radius changes of solubilized grape catechol oxidase. Phytochemistry 14 1955-1957.
14. Marques L, Fleuriet A, Cleyet-Marel J C, Macheix J J 1994 Purification of an apple polyphenoloxidase isoform resistant to SDS-proteinase K digestion. Phytochemistry 36 1117-1121.
15. Moore B M, Flurkey WH 1990 Sodium dodecyl sulfate activation of a plant polyphenoloxidase. J Biol Chem 265 4982-4988.
16. Nicolas J J, Richard-Forget F C, Goupy P M, Amiot M J, Aubert S 1994 Enzymatic browning reactions in apple and apple products. Crit Rev Food Sci Nutr 34 109-157.
17. Rathjen A H, Robinson S P 1992 Aberrant processing of polyphenol oxidase in a variegated grapevine mutant. Plant Physiol 99 1619-1625.
18. Richard-Forget F, Goupy P, Nicolas J 1995 New approaches for separating and purifying apple polyphenol oxidase isoenzymes: hydrophobic, metal chelate and affinity chromatography. J Chromatogr A667 141-153.
19. Rivas N J, Whitaker J R 1973 Purification and some properties of two polyphenol oxidases from Bartlett pears. Plant Physiol 52 501-507.
20. Robinson S P, Loveys B R, Chacko E K 1993 Polyphenol oxidase enzymes in the sap and skin of mango fruit. J Plant Physiol 20 99-107.
21. Sanchez-Ferrer A, Villalba J, Garcia-Carmona F 1989a Triton X-114 as a tool for purifying spinach polyphenol oxidase. Phytochemistry 28 1321-1325.
22. Sanchez-Ferrer A, Bru R, Garcia-Carmona F 1989b Novel procedure for extraction of a latent grape polyphenol oxidase using temperature-induced phase separation in Triton X114. Plant Physiol 91 1481-1487.
23. Sanchez-Ferrer A, Laveda F, Garcia-Carmona F 1993 Substrate-dependent activation of latent potato leaf polyphenol oxidase by anionic surfactants. J Agric Food Chem 41 1583-1586.
24. Siddiq M, Cash J N, Sinha N K, Akhter P 1994 Characterization and inhibition of PPO from pears (Pyrus communis L, cv Bosc and red). Biochem J 17 327-337.
25. Siegelman H W 1955 Detection and identification of polyphenoloxidase substrates in apple and pear skins Arch Biochem Biophys 56 97-102.
26. Smith D M, Montgomery M W 1985 Improved methods for the extraction of polyphenoloxidase from d'Anjou pears. Phytochemistry 24 901-904.
27. Söderhäll I 1995 Properties of carrot polyphenoloxidase. Phytochemistry 39 33-38.
28. Tate J N, Lun B S, York GK 1964 Polyphenoloxidase in Bartlett pears. J Food Sci 29 829-836.
29. Wang Z, Taylor K D A, Yan T 1994 Further studies on the roles of proteases in the activation of phenolase from Norway lobster (Nephrops norvegicus). Food Chem 51 99-103.
30. Whitaker 1995 Polyphenol oxidase. In: Food Enzymes, Structure and Mechanism. Chapman & Hall, New York, USA, pp 271-303.
31. Wissemann K W, Montgomery M W 1985 Purification of d'Anjou pear (pyrus communis L) polyphenol oxidase. Plant Physiol 78 256-262.
32. Zawistowski J, Biliaderis C G, Eskin N A M 1991 Polyphenol oxidase. In: Oxidative Enzymes in Foods, eds Robinson D S & Eskin N A M. Elsevier, London, UK, pp 217-274.
33. Zhou H W, Feng X 1991 Polyphenol oxidase from Yali pear (Pyrus bretschneider). J Sci Food Agric 57 307-313.