Histochemical staining following LacZ gene transfer underestimates transfection efficiency

Human Gene Therapy

Volumn 8, Issue 8, 1997, Pages 929-934

  Couffinhal, Thierry ^a   Kearney, Marianne ^a   Sullivan, Alison ^a   Silver, Marcy ^a   Tsurumi, Yukio ^a   Isner, Jeffrey M ^a,b  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b ST ELIZABETH'S MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; VIRUS VECTOR;

ADENOVIRUS; ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DNA TRANSFECTION; ENZYME ACTIVITY; FEMALE; GENE EXPRESSION; GENE TRANSFER; HISTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; LACTOSE OPERON; MICROSCOPY; MOUSE; NONHUMAN; RABBIT; SKELETAL MUSCLE; STAINING;

EID: 0030911285     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/hum.1997.8.8-929     Document Type: Article

References (25)

1. BONNEROT, C., ROCANCOURT, D., BRIAND, P., and GRIMBER, G. (1987). A beta-galactosidase hybrid protein targeted to nuclei as a marker for developmental studies. Proc. Natl. Acad. Sci. USA 84, 6795-6799.
2. FELDMAN, L.F., PASTURE, C.J., AUBAILLY, N., KEARNEY, M., CHEN, D., PERRICAUDET, M., STEG, P.G., ISNER, J.M. (1997). Improved efficiency of arterial gene transfer by use of poloxamer 407 as a vehicle for adenoviral vectors. Gene Ther. 4, 189-198.
3. FIERING, S.N., ROEDERER, M., NOLAN, G.P., MICKLEM, D.R., PARKS, D.R., and HERZENBERG, L.A. (1991). Improved FACS-Ga1: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs. Cytometry 12, 291-301.
4. FIRE, A. (1992). Histochemical techniques for locating Escherichia coli beta-galactosidase activity in transgenic organisms. Genet. Anal. Tech. Appl. 9, 151-158.
5. GORMAN, C.M., MOFFAT, L.F., and HOWARD, B.H. (1982). Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2, 1044-1051.
6. 165. Lancet 348, 370-374.
7. ISNER, J.M., WALSH, K., ROSENFIELD, K., SCHAINFELD, R., ASAHARA, T., HOGAN, K., and PIECZEK, A. (1996b). Arterial gene therapy for restenosis. Hum. Gene Ther. 7, 989-1011.
8. ISNER, J.M., WALSH, K., SYMES, J., PIECZEK, A., TAKESHITA, S., LOWRY, J., ROSENFIELD, K., WEIR, L., BROGI, E., and JURAYJ, D. (1996c). Arterial gene transfer for therapeutic angiogenesis in patients with peripheral artery disease. Hum. Gene Ther. 7, 959-988.
9. LOSORDO, D.W., PICKERING, J.G., TAKESHITA, S., LECLERC, G., GAL, D., WEIR, L., KEARNEY, M., JEKANOWSKI, J., and ISNER, J.M. (1994). Use of the rabbit ear artery to serially assess foreign protein secretion after site specific arterial gene transfer in vivo: Evidence that anatomic identification of successful gene transfer may underestimate the potential magnitude of transgene expression. Circulation 89, 785-792.
10. MACGREGOR, G.R., MOGG, A.E., BURKE, J.F., and CASKEY, C.T. (1987). Histochemical staining of clonal mammalian cell lines expression E. Coli beta galactosidase indicates heterogeneous expression of the bacterial gene. Somat. Cell. Mol. Genet. 13, 253-265.
11. MUHLHAUSER, J., PILI, R., MERRILL, M., MAEDA, H., PASSANITI, A., CRYSTAL, R., and CAPOGROSSI, M.C. (1995). In vivo angiogenesis induced by recombinant adenovirus vectors coding either for secreted or nonsecreted forms of acidic fibroblast growth factor. Hum. Gene Ther. 6, 1457-1465.
12. MULLIGAN, R.C. (1993). The basic science of gene therapy. Science 260, 926-932.
13. NABEL, E.G., YANG, Z., LIPTAY, S., SAN, H., GORDON, D., HAUDENSCHILD, C.C., and NABEL, G.J. (1993a). Recombinant platelet-derived growth factor B gene expression in porcine arteries induces intimal hyperplasia in vivo. J. Clin. Invest. 91, 1822-1829.
14. NABEL, E.G., YANG, Z.Y., PLAUTZ, G., FOROUGH, R., ZHAN, X., HAUDENSCHILD, C.C., MACIAG, T., and NABEL, G.J. (1993b). Recombinant fibroblast growth factor-1 promotes intimal hyperplasia and angiogenesis in arteries in vivo. Nature 362, 844-846.
15. NOLAN, G.P., FIERING, S., NICOLAS, J-F., and HERZENBERG, L.A. (1988). Fluorescence-activated cell analysis and sorting of viable mammalian cells based on b-D-galactosidase activity after transduction of Escherichia coli lacZ. Proc. Natl. Acad. Sci. USA 85, 2603-2607.
16. PALDI, A., DELTOUR, L., and JAMI, J. (1993). cis effect of lacZ sequences in transgenic mice. Transgenic Res. 2, 325-329.
17. RIESSEN, R., RAHIMIZADEH, H., BLESSING, E., TAKESHITA, S., BARRY, J.J., and ISNER, J.M. (1993). Arterial gene transfer using pure DNA applied directly to a hydrogel-coated angioplasty balloon. Hum. Gene Ther. 4, 749-758.
18. RIOS, C.D., OOBOSHI, H., PIEGORS, D., DAVIDSON, B.L., and HEISTAD, D.D. (1995). Adenovirus-mediated gene transfer to normal and atherosclerotic arteries. A novel approach. Arterioscler. Thromb. Vasc. Biol. 15, 2241-2245.
19. STRATFORD-PERRICAUDET, L.D., MAKEH, I., PERRICAUDET, M., and BRIAND, P. (1992). Widespread long-term gene transfer to mouse skeletal muscles and heart. J. Clin. Invest. 90, 626-630.
20. TAKESHITA, S., TSURUMI, Y., COUFFINHAL, T., ASAHARA, T., BAUTERS, C., SYMES, J.F., FERRARA, N., and ISNER, J.M. (1996). Gene transfer of naked DNA encoding for three isoforms of vascular endothelial growth factor stimulates collateral development in vivo. Lab. Invest. 75, 487-502.
21. TRIPATHY, S.K., SVENSSON, E.C., BLACK, H.B., GOLDWASSER, E., MARGALITH, M., HOBART, P.M., and LEIDEN, J.M. (1996). Long-term expression of erythropoietin in the systemic circulation of mice after after intramuscular injection of a plasmid DNA vector. Proc. Natl. Acad. Sci. USA 93, 10876-10880.
22. ULMER, J.B., DONNELLY, J.J., PARKER, S.E., RHODES, G.H., FELGNER, P.L., DWARKI, V.J., GRAMKOWSKI, S.H., DECK, R.R., DEWITT, C.M., FRIEDMAN, A., HAWE, L.A., LEANDER, K.R., MARTINEZ, D., PERRY, H.C., SHIVER, J.W., MONTGOMERY, D.L., and LIU, M.A. (1993). Heterologous protection against influenza by injection of DNA encoding a viral protein. Science 259, 1745-1749.
23. WILSON, J. (1993). Vehicles for gene therapy. Nature 365, 691-692.
24. WOLFF, J.A., MALONE, R.W., WILLIAMS, P., CHONG, W., ACSADI, G., JANI, A., and FELGNER, P.L. (1990). Direct gene transfer into mouse muscle in vivo. Science 247, 1465-1468.
25. ZABNER, J., COUTURE, L.A., SMITH, A.E., and WELSH, M.J. (1994). Correction of cAMP-stimulated fluid secretion in cystic fibrosis airway epithelia: efficiency of adenovirus-mediated gene transfer in vitro. Hum. Gene Ther. 5, 585-593.