An improved and combined reverse transcription-polymerase chain reaction assay for reliable detection of metastatic melanoma cells in peripheral blood

Melanoma Research

Volumn 7, Issue 2, 1997, Pages 111-116

  Hanekom, G S ^a   Johnson, C A ^a   Kidson, S H ^a  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

contamination; illegitimate transcription; metastatic melanoma; reverse transcription polymerase chain reaction; tyrosinase

Indexed keywords

MESSENGER RNA; MONOPHENOL MONOOXYGENASE;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DIAGNOSTIC ACCURACY; HUMAN; HUMAN CELL; LABORATORY DIAGNOSIS; MELANOMA CELL; METASTASIS; PRIORITY JOURNAL; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0030909378     PISSN: 09608931     EISSN: None     Source Type: Journal    
DOI: 10.1097/00008390-199704000-00004     Document Type: Article

References (14)

1. Smith B, Selby P, Southgate J, et al. Detection of melanoma cells in peripheral blood by means of reverse transcriptase and polymerase chain reaction. Lancet 1991; 338: 1227-1229.
2. Tobal K, Sherman LS, Foss AJE, et al. Detection of melanocytes from uveal melanoma in peripheral blood using the polymerase chain reaction. Invest Ophthalmol Vis Sci 1993; 34: 2622-2625.
3. Battyani Z, Xerri L, Hassoun J, et al. Tyrosinase gene expression in human tissues. Pigment Cell Res 1993; 6: 400-405.
4. Wang X, Heller R, VanVoorhis N, et al. Detection of submicroscopic lymph node metastases with polymerase chain reaction in patients with malignant melanoma. Ann Surg 1994; 220: 768-774.
5. Brossart P, Keilholz U, Scheibenbogen C, et al. Detection of residual tumor cells in patients with malignant melanoma responding to immunotherapy. J Immunother 1994; 15: 38-41.
6. Foss AJE, Guille MJ, Occleston NL, et al. The detection of melanoma cells in peripheral blood by reverse transcriptionpolymerase chain reaction. Br J Cancer 1995; 72: 155-159.
7. Battayani Z, Grob JJ, Xerri L, et al. Polymerase chain reaction detection of circulating melanocytes as a prognostic marker in patients with melanoma. Arch Dermatol 1995; 131: 443-447.
8. Hoon DS, Wang Y, Dale PS, et al. Detection of occult melanoma cells in blood with a multiple-marker polymerase chain reaction assay. J Clin Oncol 1995; 13: 2109-2116.
9. Brossart P, Schmier JW, Kruger S, et al. A polymerase chain reaction-based semiquantitative assessment of malignant melanoma cells in peripheral blood. Cancer Res 1995; 55: 4065-4068.
10. Chelly J, Concordet JP, Kaplan JC, et al. Illegitimate transcription: transcription of any gene in any cell type. Proc Natl Acad Sci USA 1989; 86: 2617-2621.
11. Hoal-Van Helden FG, Wilson EL, Dowdle EB. Characterization of seven human melanoma cell lines: melanogenesis and secretion of plasminogen activators. Br J Cancer 1986; 54: 287-295.
12. Xie W, Rothblum LI. Rapid, small-scale RNA isolation from tissue culture cells. BioTechiques 1991; 11: 325-327.
13. Taylor JJ, Heasman PA. Control genes for reverse transcription/polymerase chain reaction (RT-PCR). Br J Haematol 1994; 86: 444-447.
14. Belák S, Ballagi-Pordány A. Experiences on the application of the polymerase chain reaction in a diagnostic laboratory. Mol Cell Probes 1993; 7: 241-248.