The isolated comet tail pseudopodium of Listeria monocytogenes: A tail of two actin filament populations, long and axial and short and random

Journal of Cell Biology

Volumn 137, Issue 1, 1997, Pages 155-167

  Sechi, Antonio S ^a   Wehland, Jürgen ^b   Small, J Victor ^a  

^a ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN FILAMENT; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL MOTILITY; IMMUNOFLUORESCENCE; LISTERIA MONOCYTOGENES; MACROPHAGE; PRIORITY JOURNAL; PSEUDOPODIUM;

ACTINS; ANIMALS; BACTERIAL PROTEINS; FLUORESCENT ANTIBODY TECHNIQUE; HELA CELLS; HUMANS; LISTERIA INFECTIONS; LISTERIA MONOCYTOGENES; MACROPHAGES; MICE; MICROSCOPY, ELECTRON; MICROSCOPY, VIDEO; PSEUDOPODIA;

EID: 0030908903     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.137.1.155     Document Type: Article

References (36)

1. Anderson, K.I., Y.-L. Wang, and J.V. Small. 1996. Coordination of protrusion and translocation of the keratocyte involves rolling of the cell body. J. Cell Biol. 134:1209-1218.
2. Brundage, R.A., G.A. Smith, A. Camilli, J.A. Theriot, and D.A. Portnoy. 1993. Expression and phosphorylation of the Listeria monocytogenes ActA protein in mammalian cells. Proc. Natl. Acad. Sci. USA. 90:11890-11894.
3. Cudmore, S., I. Reckman, G. Griffiths, and M. Way. 1996. Vaccinia virus: a model system for actin-membrane interactions. J. Cell Sci. 109:1739-1747.
4. Dabiri, G.A., J.M. Sanger, D.A. Portnoy, and F.S. Southwick. 1990. Listeria monocytogenes moves rapidly through the host-cell cytoplasm by inducing directional actin assembly. Proc. Natl. Acad. Sci. USA. 87:6068-6072.
5. Dold, F.G., J.M. Sanger, and J.W. Sanger. 1994. Intact alpha-actinin molecule are needed for both the assembly of actin into the tails and the locomotion of Listeria monocytogenes inside infected cells. Cell Motil. Cytoskeleton. 28:97-107.
6. Domann, E., J. Wehland, M. Rohde, S. Pistor, M. Hartl, W. Goebel, M. Leimeister-Wächter, M. Wuenscher, and T. Chakraborty. 1992. A novel bacterium virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin. EMBO (Eur. Mol. Biol. Organ.) J. 11:1981-1990.
7. Franck, Z., R. Gary, and A. Bretscher. 1993. Moesin, like ezrin, colocalizes with actin in the cortical cytoskeleton in cultured cells, but its expression is more variable. J. Cell Sci. 105:219-231.
8. Heinzen, R.A., S.F. Hayes, M.G. Peacock, and T. Hackstadt. 1993. Directional actin polymerization associated with spotted fever group rickettsia infection of Vero cells. Infect. Immun. 61:1926-1935.
9. Herzog, M., A. Draeger, E. Ehler, and J.V. Small. 1994. Immunofluorescence microscopy of the cytoskeleton: Double and triple immunofluorescence. In Cell Biology: A Laboratory Handbook. J.E. Celis, editor. Academic Press, San Diego. 355-360.
10. Ishikawa, H., R. Bischoff, and H. Holtzer. 1969. Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J. Cell Biol. 43:312-328.
11. Kocks, C. E. Gouin, M. Tabouret, P. Merche, H. Ohayon, and P. Cossart. 1992. L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein. Cell. 68:521-531.
12. Kocks, C., J.-B. Marchand, E. Gouin, H. D'Hautville, P.J. Sansonetti, M.-F. Carlier, and P. Cossart. 1995. The unrelated surface proteins ActA of Listeria monocytogenes and IcsA of Shigella flexneri are sufficient to confer actin-based motility on Listeria innocua and Escherichia coli, respectively. Mol. Microbiol. 18:413-423.
13. Lasa, I., and P. Cossart. 1996. Actin-based bacterial motility: towards a definition of the minimal requirements. Trends Cell Biol. 6:109-114.
14. Maupin-Szamier, P., and T.D. Pollard. 1978. Actin filament destruction by osmium tetroxide. J. Cell Biol. 77:837-852.
15. McKenna, N.M., and Y.-L. Wang. 1989. Culturing cells on the microscope stage. Methods Cell Biol. 29:295-305.
16. Mogilner, A., and G. Oster. 1996. Cell motility driven by actin polymerization. Biophys. J. 71:3501-3510.
17. Nanavati, D., F.T. Ashton, J.M. Sanger, and J.W. Sanger. 1994. Dynamics of actin and alpha-actinin in the tails of Listeria monocytogenes in infected PtK2 cells. Cell Motil. Cytoskeleton. 28:346-358.
18. Pollard, T.D. 1995. Missing link for intracellular bacterial motility? Curr. Biol. 5:837-840.
19. Sanger, J.M., J.W. Sanger, and F.S. Southwick. 1992. Host cell actin assembly is necessary and likely to provide the propulsive force for intracellular movement of Listeria monocytogenes. Infect. Immun. 60:3609-3619.
20. Sechi, A., and J.V. Small. 1997. Isolation of the actin comet tails of Listeria monocytogenes. In Cell Biology: A Laboratory Handbook II. J.E. Celis, editor. Academic Press, San Diego. In press.
21. Small, J.V. 1981. Organization of actin in the leading edge of cultured cells: influence of osmium tetroxide and dehydration on the ultrastructure of actin meshworks. J. Cell Biol. 91:695-705.
22. Small, J.V. 1985. Factors affecting the integrity of actin meshworks in cultured cells. In Cell Motility II. Proc. Yamada Conf. on Cell Motility. H. Ishikawa, S. Hatano, and H. Sato, editors. Nagoya, 1985. 493-506.
23. Small, J.V. 1988. The actin cytoskeleton. Electron Microsc. Rev. 1:155-174.
24. Small, J.V., and M. Herzog. 1994. Whole mount electron microscopy of the cytoskeleton: negative staining methods. In Cell Biology: A Laboratory Handbook. J.E. Celis, editor. Academic Press, San Diego. 135-139.
25. Smith, G.A., D.A. Portnoy, and J.A. Theriot. 1995. Asymmetric distribution of the Listeria monocytogenes ActA protein is required and sufficient to direct actin-based motility. Mol. Microbiol. 17:945-951.
26. Southwick, F.S., and D.L. Purich. 1996. Intracellular pathogenesis of Listeriosis. N. Engl. J. Med. 334:770-776.
27. Theriot, J.A. 1995. The cell biology of infection by intracellular bacterial pathogens. Annu. Rev. Cell Dev. Biol. 11:213-239.
28. Theriot, J.A., and T.J. Mitchison. 1991. Actin microfilament dynamics in locomoting cells. Nature (Lond.). 352:126-131.
29. Theriot, J.A., T.J. Mitchison, L.G. Tilney, and D.A. Portnoy. 1992. The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization. Nature (Lond.). 357:257-260.
30. Theriot, J.A., J. Rosenblatt, D.A. Portnoy, P.J. Goldschmidt-Clermont, and T.J. Mitchison. 1994. Involvement of profilin in the actin-based motility of Listeria monocytogenes in cells and cell-free extracts. Cell. 76:505-517.
31. Tilney, G.T., and D.A. Portnoy. 1989. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes. J. Cell Biol. 109:1597-1608.
32. Tilney, L.G., and M.S. Tilney. 1993. The wily ways of a parasite: induction of actin assembly by Listeria. Trends Microbiol. 1:25-31.
33. Tilney, L.G., D.J. DeRosier, and M.S. Tilney. 1992a. How Listeria exploits host cell actin to form its own cytoskeleton. I. Formation of a tail and how that tail might be involved in movement. J. Cell Biol. 118:71-81.
34. Tilney, L.G., D.J. DeRosier, A. Weber, and M.S. Tilney. 1992b. How Listeria exploits host cell actin to form its own cytoskeleton. II. Nucleation, actin filament polarity, filament assembly, and evidence for a pointed end capper. J. Cell Biol. 118:83-93.
35. Zeile, W.L., D.L. Purich, and F.S. Southwick. 1996. Recognition of two classes of oligoproline sequences in profilin-mediated acceleration of actin-based Shigella motility. J. Cell Biol. 133:49-59.
36. Zhukarev, V., F. Ashton, J.M. Sanger, J.W. Sanger, and H. Shuman. 1995. Organization and structure of actin filament bundles in Listeria-infected cells. Cell Motil. Cytoskeleton. 30:229-246.