Bahamamide, an unusual cyclic bis-amide produced by an undescribed marine bacterium

Natural Product Letters

Volumn 10, Issue 1, 1997, Pages 75-78

  Boehler, Mark ^a   Jensen, Paul R ^a   Fenical, William ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Bahamamide; Cyclic bis amide; Marine bacterial metabolites

Indexed keywords

AMIDE; BAHAMAMIDE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM; BACTERIUM CULTURE; CHEMICAL STRUCTURE; CULTURE MEDIUM; GRAM NEGATIVE BACTERIUM; NONHUMAN; SEA; SEDIMENT;

EID: 0030907812     PISSN: 10575634     EISSN: None     Source Type: Journal    
DOI: 10.1080/10575639708043699     Document Type: Article

References (5)

1. W. Fenical, Chem. Rev. 1993, 95, 1673.
2. This isolate, strain CNE-852, was analyzed by FAME analysis (Acculab, Newark, Delaware). The closest similarity was with the genus Vibrio, (similiarity index of 0.166), however this is a very poor genus correlation. Strain CNE-852 is a highly motile, Gram-negative, curved rod that does not grow when seawater is replaced with deionized water.
3. To isolate this strain, the sediment was serially diluted with sterile seawater and plated onto an agar medium (B3: peptone 1%, yeast extract 0.6%, glycerol 0.6%, seawater 75%, de-ionized water 25%, 1.6% agar). The strain was removed from the isolation plate with a sterile loop and obtained in pure culture following successive transfers on B3 agar. For large scale production, strain CNE-852 was grown in Medium B3, without agar, at a shaker speed of 230 rpm, for 7 days. The whole fermentation broth was subsequently extracted with ethyl acetate to obtain the crude extract.
4. 2.
5. T. Kameyama, A. Takahashi, S. Kurosawa, M. Ishizuka, Y. Okami, T. Takeuchi, and H. Umezawa, J. Antibiot. 1987, 40, 1664.