Reversal of the nucleotide specificity of ketol acid reductoisomerase by site-directed mutagenesis identifies the NADPH binding site

Archives of Biochemistry and Biophysics

Volumn 338, Issue 1, 1997, Pages 83-89

  Rane, Madhavi J ^a   Calvo, K C ^b  

^a University of Louisville School of Medicine   (United States)

^b UNIVERSITY OF AKRON   (United States)

Author keywords

enzyme engineering; nucleotide binding site; reductoisomerase

Indexed keywords

ISOMERASE; KETOL; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GROWTH; BINDING AFFINITY; BINDING SITE; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETIC ENGINEERING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEADY STATE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FELIS CATUS;

EID: 0030906828     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1006/abbi.1996.9802     Document Type: Article

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